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  • 2000-2004  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Colocalization of DNA Fragmentation and Caspase-3 Activation During Atresia in Pig Antral Follicles (2004)
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 33 (2004), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Apoptosis is the cellular mechanism of ovarian follicular atresia. The major downstream effector of this phenomenon in many tissues is caspase-3 but little is known about its role in pig ovarian apoptosis. In the present study, we detected the localization of caspase-3 in parallel with nuclear fragmentation (TUNEL) on healthy and early atretic antral follicles. In healthy antral follicles caspase-3 and TUNEL positivity were occasionally recorded within theca layer. The incidence of DNA fragmentation, as indicated also by the biochemical detection, increased mainly in the granulosa layer of early atretic follicles. Quantitative analysis revealed, besides, that atresia was accompanied by a higher incidence of caspase-3 (57.20 ± 20.05 versus 3.64 ± 0.61 positive cells in atretic versus healthy follicles, respectively; P 〈 0.05), of TUNEL positivity (20.13 ± 9.33 versus 0.42 ± 0.12; P 〈 0.05) and simultaneous immunostaining for caspase-3 and TUNEL (15.02 ± 6.95 versus 0.31 ± 0.05; P 〈 0.05) in the granulosa layer. In detached granulosa cells isolated from the follicular fluid of early atretic follicles a further significantly increase was recorded in the percentage of TUNEL positivity and in the incidence of cells that showed colocalization of caspase-3 activity and DNA fragmentation. Granulosa cells of early atretic follicles exhibited a higher positivity for caspase-3 localized in the cytoplasm and occasionally in the nucleus area of granulosa cells. These results indicate that capsase-3 was involved and precociously activated during the process of atresia. Finally, the progressively higher incidence of TUNEL positivity and of double immunostaining in atretic cells collected within the follicular fluid seems to indicate that proteases activity leads only tardily in a detectable DNA fragmentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.2004.00504.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    What have we learnt from gastric biopsy? (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Alimentary pharmacology & therapeutics 17 (2003), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Pathologists would claim that the most reliable information obtained on gastric diseases is pathology-based. This manuscript summarizes the four major biopsy-based contributions to the current knowledge of non-neoplastic gastric diseases: (i) the in vivo definition of gastritis; (ii) the recognition of the clinicopathological patterns of gastritis; (iii) the morphological links between gastritis and stomach cancer; and finally (iv) the recent information on the possible reversibility of early or advanced precancerous gastric lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2036.17.s2.15.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of Muskmelon Fruit Peroxidases at Different Developmental Stages (2000)
    Springer
    Biologia plantarum 43 (2000), S. 373-379 
    Details
    ISSN: 1573-8264
    Keywords: Cucumis melo ; chromatography ; electrophoresis ; host-plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An increase in exocarp peroxidase activity was observed in fruit at 5 to 30 days post pollination (DPP), and decreased at 40 and 50 DPP. Total peroxidase activity of the mesocarp was significantly lower than the exocarp in all developmental stages. Mesocarp peroxidase activity decreased consecutively from outer, to middle and, to inner tissue at every developmental stage. Total activity in the mesocarp peaked at 20 DPP. Native-PAGE of exocarp tissue showed at least two cathodic (basic) peroxidases and two anionic (acidic) peroxidases. The number of isozymes was greatest and bands most intense at 30 DPP. IEF-PAGE of the 5 to 50 DPP fruit exocarp showed at least 8 peroxidase isozymes (pI 4.6 to 9.6). Anion exchange chromatography showed only one peak of anionic peroxidase activity that was not evident until 15 DPP. This peak was greatest at 30 DPP and declined at 40 and 50 DPP. Cationic peroxidase isozymes appeared to be the predominant and most intense isoforms throughout fruit development. The changes in peroxidase activity corresponded to fruit formation and may be associated with susceptibility to fruit rot.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026777907099
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    Paper (German National Licenses)
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