Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Experimental dermatology 10 (2001), S. 0 
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The production and its induction by ultraviolet radiation (UVR) of proopiomelanocortin (POMC)-derived peptides by keratinocytes has been reported, albeit not consistently. Recently we demonstrated that only under specific culturing conditions human keratinocytes are capable of producing a β-endorphin (βE)-like peptide with the characteristics of β-lipotropin (βLPH). Here the presence and UV-induction of βE-immunoreactivity (βE-IR) in keratinocytes in human skin in vivo was investigated. βE-IR was detectable by immunohistochemistry in keratinocytes of the follicular matrix and to some extent in cells of sweat ducts, but was absent from epidermal keratinocytes. Absence of βE-IR was confirmed by radioimmunoassay of HPLC-fractionated extracts of normal epidermis. Repeated exposure to solar-simulated UVR had no effect. This investigation is the first to demonstrate the presence of βE-immunoreactive material in the follicular matrix of corporal hairs and in duct cells of sweat glands. The possible meaning of these results is discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Plant pathology 50 (2001), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 4
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 5
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Four forms of Colletotrichum representing three distinct virulence phenotypes were found associated with foliar anthracnose of yam in Nigeria: the highly virulent (= severity of disease) slow-growing grey (SGG); the moderately virulent fast-growing salmon (FGS); the weakly virulent fast-growing grey (FGG); and the moderately virulent fast-growing olive (FGO) morphotype. Isolates of the four forms were identified as C. gloeosporioides, based on morphology. The reaction of monoconidial cultures on casein hydrolysis medium (CHM), PCR-RFLP and sequence analysis of the internal transcribed spacer region of the ribosomal DNA (ITS1-5·8S-ITS2) were used to establish the identity of the yam anthracnose pathogen(s). All yam isolates were distinguished from C. acutatum by the absence of protease activity on CHM. On ITS PCR and enzymatic digestion of PCR products, all FGS, FGO and SGG isolates produced RFLP patterns identical to those of C. gloeosporioides reference isolates, while FGG isolates revealed unique ITS RFLP banding patterns. Sequence analysis of the ITS1 region and of the entire ITS region revealed that SGG, FGS and FGO isolates were highly similar (98–99% nucleotide identity) and showed 97–100% identity to C. gloeosporioides. Less than 93% similarity of these fungal isolates to reference C. acutatum and C. lindemuthianum isolates was observed. The molecular study confirmed that foliar anthracnose of yam is caused by C. gloeosporioides. While a high similarity was found among most C. gloeosporioides fungi from yam, isolates of the FGG form did not cluster with any previously described Colletotrichum species, and probably represent a distinct species.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 6
    ISSN: 1432-0533
    Keywords: Key words Glioma ; Multidrug resistance ; Chemotherapy ; Endothelial ; Blood brain barrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Human malignant gliomas are commonly resistant to chemotherapy. Here, we examined the role of the multidrug resistance (mdr) mechanism in the chemoresistance of these tumors, using a twofold approach: (i) by assessing a possible mdr phenotype before and after chronic drug exposure of glioma cells in vitro, and (ii) by assessing the modulation of expression of the mdr-associated P-glycoprotein (Pgp) using radiotherapy and serial cycles of chemotherapy in human glioblastoma patients in vivo. T98G, and to a lesser degree, LN-229 human malignant glioma cells exhibit a constitutive mdr phenotype as determined by the modulation of dye transport and by the augmentation of chemosensitivity by the mdr antagonist, verapamil. Thus, coexposure to verapamil enhances the cytotoxicity of vincristine, doxorubicin and VM26 in T98G cells and that of vincristine in LN-229 cells. Chronic exposure of the cells to low concentrations of vincristine and doxorubicin, but not VM26, topotecan or BCNU, moderately enhances the mdr-like phenotype, as assessed by drug expulsion assays. However, chronic exposure to increasing drug concentrations does not significantly alter the sensitivity to the respective drugs. These data are consistent with a constitutive, but not drug-inducible, mdr-like drug resistance in glioma cells in vitro. Immunocytochemical analysis of human malignant gliomas in vivo reveals that Pgp expression is more abundant in endothelial cells within the gliomas, than in the glioma cells proper. Importantly, Pgp expression is unaltered by radiochemotherapy, assessed by comparative immunocytochemistry of glioma specimens obtained serially before and after radiochemotherapy. We conclude that (i) glioma cells exhibit constitutive mdr-like drug resistance that is not significantly altered by chronic drug exposure in vitro; (ii) endothelial cells may play an important role in Pgp-mediated drug resistance of gliomas in vivo; (iii) radiotherapy and repeated chemotherapy cycles do not modulate Pgp expression in human malignant gliomas in vivo; (iv) there is preliminary evidence for a non-Pgp, verapamil-sensitive drug transport activity in glioma cells.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 7
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Cycles of protein phosphorylation are fundamental in regulating the progression of the eukaryotic cell through its division cycle. Here we test the complement of Drosophila protein kinases (kinome) for cell cycle functions after gene silencing by RNA-mediated interference. We observed cell cycle ...
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Reproduktionsmedizin 16 (2000), S. 140-146 
    ISSN: 1434-808X
    Keywords: Schlüsselwörter Fortpflanzungsmedizin • Genetik • Präimplantationsdiagnostik • Bundesärztekammer • Europäische Integration ; Keywords Reproductive medicine • Genetics • Preimplantation genetic diagnosis • German Medical Association • European integration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Abstract Besides an exponential growth in possibilities for diagnosis, treatment, and prevention in reproductive medicine and genetics, the synergistic effects of modern health technologies have led to the necessity of fast transfer of knowledge into the public domain in order to develop a common social consensus. Thus, physicians and scientists have enormous obligations not only for the development of medical technology but also of medical and societal ethics. Very early, i. e. in 1984, the German Medical Association developed professional laws for reproductive medicine. In addition, the German government saw the need in 1990 for an „Embryo Protection Law” (Embryonenschutzgesetz). Today, in the light of European integration, the medical profession's and state's obligations are challenged in different ways. Whereas the German Medical Association continuously works on professional guidelines for reproductive medicine and genetics – currently the case with preimplantation genetic diagnosis – the government must develop the sociolegal framework for ethically sound reproductive medicine, i. e. , in the areas of inheritance law, adoption law, family law, and law of succession, the latter especially in the case of discrepancies between genetic vs. social paternity in heterologous insemination.
    Notes: Zusammenfassung Die Synergieeffekte moderner Medizintechnologien in Fortpflanzungsmedizin und Genetik führen neben exponentiellem Wachstum der Möglichkeiten in Diagnostik, Therapie und Prävention zur Notwendigkeit eines schnellen Wissenstransfers in die Öffentlichkeit, um eine gesamtgesellschaftliche Konsensbildung herbeizuführen. Ärzten und Wissenschaftlern kommt somit eine entscheidende Funktion nicht nur in der Fortentwicklung der Medizintechnik, sondern auch der Medizin- und Sozialethik zu. Die Bundesärztekammer hat die Fortpflanzungsmedizin schon 1984 berufsrechtlich geregelt und seitdem ständig begleitet, darüber hinaus wurde vom Gesetzgeber 1990 die Notwendigkeit gesehen, eine bundesgesetzliche Regelung durch das Embryonenschutzgesetz zu schaffen. Heute, 10 Jahre später, sind Ärzteschaft und Staat angesichts des gesamteuropäischen Integrationsprozesses in unterschiedlichem Maße gefordert. Während die Ärzteschaft kontinuierlich – wie jetzt für die Präimplantationsdiagnostik – das Berufsrecht zu Fortpflanzungsmedizin und Genetik aktualisiert, ist es Sache des Bundesgesetzgebers, die primär sozialrechtlich erforderlichen Rahmenbedingungen für eine ethisch vertretbare Fortpflanzungsmedizin – v. a. für Abstammungsrecht, Adoptionsvermittlungsgesetz, sowie familien- und erbrechtliche Fragen bei Auseinanderfallen genetischer und sozialer Elternschaft bei heterologer Befruchtung – zu entwickeln.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...