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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    Journal of fish biology 63 (2003), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A pluripotent cell line, sea bream embryonic stem-like cells (SBES1), was developed from blastula-stage embryos of the cultured red sea bream, Chrysophrys major. The SBES1 cells were cultivated in Dulbecco's modified eagles medium (DMEM) medium supplemented with foetal bovine serum, marine fish serum, fish embryo extract, selenium, basic fibroblast growth factor and leukemia inhibitory factor. They were small and round or polygonal, and grew actively and stabely in culture. The cells exhibited a positive alkaline phosphatase activity upon histochemical staining. When the cells were treated with all-trans retinoic acid, they differentiated into various types including neuron-like, neuroglia-like and muscle-like cells, suggesting that the SBES1 cells remained pluripotent in culture. Chromosome analysis revealed that SBES1 cells had a normal diploid karyotype with 2n = 2st + 46t. At present, SBES1 cells have been cultured for 〉180 days with more than 60 passages. High survival rate has been obtained after cryopreservation of cell cultures. This embryonic cell line may potentially be used for the production of transgenic red sea bream.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Neural computing & applications 9 (2000), S. 266-279 
    ISSN: 1433-3058
    Keywords: Keywords: Browsing facility; Information visualisation; Self-organising map; Software library; Software retrieval; Software reuse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: This paper presents an approach to self-structuring software libraries. The authors developed a representation scheme to construct a feature space over a collection of software assets. The feature space is represented and classified by a variety of the self-organising map, called the Nested Software Self-Organising Map (NSSOM), consisting of a top map and a set of sub-maps nested in the top map. The clustering on the top map provides general improvements in retrieval recall, while the lower-level nested maps further elaborate the clusters into more specific groups enhancing retrieval precision. The results of preliminary evaluation showed that NSSOM is capable of enhancing precision without sacrificing recall. In addition, a user-friendly browsing facility has also been developed which helps users predict the desired components by providing an intelligible search space. The present approach attempts to achieve an optimal combination of efficiency, accuracy and user-friendliness, which is not offered by the existing software retrieval systems.
    Type of Medium: Electronic Resource
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