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  • 1995-1999  (3)
  • 1990-1994  (4)
  • 1985-1989  (4)
  • 1970-1974  (5)
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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 47 (1986), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Catecholamine turnover in brain areas innervated by dopaminergic neurons was examined 2, 6, and 12 days after bilateral, N-methyl-D-aspartate lesions confined to the rat medial prefrontal cortex. The lesion produced a significant regional increase in the concentration of 3,4-dihydroxyphenylethylamine (DA, dopamine) in both the medial prefrontal cortex and the ventral tegmental area. DA concentrations were increased in the nucleus accumbens on day 6 (128% of control), in the ventral tegmental area on day 2 (130% of control), and in the medial prefrontal cortex on days 2 (145% of control) and 6 (127% of control). The only significant changes in the concentration of 3,4-dihydroxyphenylacetic acid (DOPAC) (197% of control), and in the ratio DOPAC/DA (163% of control) were found in the medial prefrontal cortex on day 6 post-lesion. All parameters had returned to control levels by day 12. DA depletion after the administration of α-methyl-p-tyrosine (AMPT) was not significantly different between excitotoxin-lesioned and sham animals on day 6 in all brain regions. Noradrenaline (NA) and 3,4-dihydroxyphenylethyleneglycol concentrations and their ratios, and the depletion of noradrenaline after AMPT were also determined, and the lesion resulted in a significant regional increase in NA in both the nucleus accumbens and the ventral tegmental area. An elevation of NA (147% of control) in the nucleus accumbens was found on day 12. Since the excitotoxin lesion destroys corticofugal efferents from medial prefrontal cortex to the nucleus accumbens, the anterior corpus striatum and the ventral tegmental area, our results provide no evidence for a role of these cortical projections in the regulation of subcortical DA metabolism. Corticofugal efferents from the medial prefrontal cortex do appear to exert a modulatory effect over NA stores of the nucleus accumbens.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary. A multicentre trial was conducted to compare the efficacy and side-effects of an intramuscularly administered PGE2 analogue and vacuum aspiration in women with a delay of up to 21 days in the expected onset of menses. A total of 473 such women were randomly allocated to treatment with either 16-phenoxy-W-17, 18, 19, 20-tetranor PGE2 methyl sulfonylamide (three intramuscular injections of 0.5 mg at 3-h intervals) or vacuum aspiration, and the outcome of therapy assessed 1, 2 and 6–8 weeks later. Retrospective analysis of hCG levels indicated that 419 (88.6%) women had been pregnant at the time of treatment. With few exceptions, administration of the PGE2 analogue induced vaginal bleeding in both pregnant and non-pregnant women but the duration and subjectively perceived amount of bleeding were greater than after vacuum aspiration. Both treatments were equally effective. In pregnant women the overall frequency of complete abortion was 91% for prostaglandin treatment and 94% for vacuum aspiration. If non-pregnant women were included, the respective success rates (i.e. percentages of women not pregnant 2 weeks after treatment) were 92% and 95%. Gastrointestinal side-effects and lower abdominal pain requiring intramuscular analgesia were more common after prostaglandin therapy than following vacuum aspiration in both pregnant and non-pregnant women.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 38 (1989), S. 0 
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In a series of growth room experiments, a determinate line of Vicia faba was shown to be more susceptible to infection by Ascochyta fabae than a conventional indeterminate line. On the determinate line, lesions developed more quickly on younger leaves. No evidence of systemic infection was found and disease transfer to the emerging seedling was probably by physical contact with the infected seed testa. Successful establishment of the disease required leaf wetness for periods of 4 h at 20–25° C to 12 h at 10 C. The rate of pycnidiospore germination on leaves decreased with temperature over this range, but did not differ between the determinate and indeterminate lines.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Three polymorphic markers have been used to improve the genetic map of the region Xp22.1-p22.2, which contains the HYP (hypophosphataemic rickets) locus. DXS365 gave no recombinants with HYP, with a peak Lod score of 5.4 at θ = 0.0. A microsatellite marker mPA274 was derived for the DXS274 locus; it detects five alleles with a polymorphism information content of 0.55. Combining information from this microsatellite and the original DXS274 marker, probe CRI-L1391, the peak Lod score for DXS274 against HYP was 9.6 at θ = 0.02. A microsatellite associated with the DXS207 locus (mPA207) gave a peak lod score against HYP of 4.7 at θ = 0.14. A consideration of key recombinants and multilocus analysis suggests the gene order: Xpter-DXS207-DXS43-DXS197-(DXS365, HYP)-DXS274-DXS41-Xcen.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have screened fourteen kindreds with X-linked hypophosphataemic rickets with four microsatellite markers, viz. AFM163yh2, DXS999 (AFM234yf12), DXS443 and DXS365, in order to refine the genetic map flanking the gene, and to define a close flanking interval for the construction of a yeast artificial chromosome (YAC) and cosmid contig. The genetic data were enhanced after the isolation of a large 1.2-megabase YAC derived from AFM163yh2, in which marker DXS274 was present but not DXS365 or DXS443. Against HYP, DXS365, AFM163yh2 and DXS443 showed no recombinants (Z max = 18.1, Z max = 9.9, and Z max = 16.0 respectively). DXS999 gave Z max = 9.6 at 4% recombination and lies distal to HYP but proximal to DXS197 and DXS43. The disease gene and markers AFM163yh2 and DXS365 are flanked by DXS443 and DXS274. Combining the genetic and physical data, we are able to propose the following gene marker order: Xptel-DXS43-DXS197-DXS999-DXS443-[(DXS365-AFM163yh2), HYP]-DXS274-DXS41-Xcen.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric nephrology 7 (1993), S. 232-232 
    ISSN: 1432-198X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The location of the HYP gene, which determines X-linked hypophosphataemic rickets, has been refined considerably by linkage analysis, and three new microsatellite primers isolated, Cap32 (DXS7473), Cap29 (DXS7474) and 7v2 (DXS7475). The locations of four other markers have also been determined (DXS1226, AFMa176zb1, AFMa152wc5, and AFM346azc1). Markers Cap29 and Cap32 are the closest distal markers to the gene with z max = 11.93, θ max = 0.018, and z max = 12.03, θ max = 0.015 respectively. Both Cap29 and Cap32 are proximal to DXS365 and AFMa176zb1, as deduced by screening non-chimaeric yeast artificial chromosomes (YACs) from a contig spanning the HYP gene. A single crossover places AFMa176zb1 distal to the disease gene. There are no recombinations between 7v2 and HYP (z max = 12.9, θ max = 0.0), or between 7v2 and adjacent markers Cap32, Cap29, AFMa176zb1, DXS1683 and DXS365. However screening of YAC clones encompassing the HYP gene and also P1 clones localises 7v2 distal to Cap29 and Cap32, and proximal to DXS443. Marker DXS1226 is placed outside the region containing the gene, and is located proximal to DXS274 as confirmed by a crossover for this marker and DXS41 against HYP, and its presence on YAC 83BO5. Genetic mapping of CEPH pedigrees, and screening of YACs places AFMa152wc5 and AFMa346zc1 between DXS1683 and DXS1052. The following gene marker map presents the best order for the HYP region: Xptel-DXS43-DXS999-DXS443-(DXS365/DXS7475/ AFMa176zb1)-(DXS7474/DXS7473)-HYP-DXS1683-(AFMa152wc5/AFMa346zc1)-DXS1052-DXS274-(DXS41/DXS1226)-Xcen. The distance between the cluster of distal flanking markers Cap29 (DXS7474), Cap32 (DXS7473), and DXS1683 is approximately 300 kb, as deduced from physical map data from a YAC contig spanning the gene. Thus the gene for HYP is contained within a single YAC (900AO472). Of further interest, is the location of a putative vitamin D response element (VDRE) on this YAC.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Journal of inherited metabolic disease 8 (1985), S. 53-57 
    ISSN: 1573-2665
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report the case of a boy with 5,10-methylenetetrahydrofolate reductase deficiency. The clinical features consisted of severe mental retardation, spasticity and seizures remaining static to 7 years of age followed by a phase of rapid deterioration and death at 7 1/2 years of age. The main biochemical findings were homocystinaemia, homocystinuria, a normal methionine level in plasma and cerebrospinal fluid, an increased excretion of methionine in urine and a very low level of folate in the cerebrospinal fluid. The activity of 5,10-methylenetetrahydrofolate reductase was greatly reduced in the patient's lymphocytes and liver.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Potato research 15 (1972), S. 200-208 
    ISSN: 1871-4528
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Zusammenfassung Der Mangel an geeigneten, gut definierten Markierungsgenen hat genetische Untersuchungen an Kartoffeln gehemmt. Eine Fülle potentiell möglicher genetischer Variation ist in den selbstunverträglichen kultivierten diploiden Formen vonSolanum tuberosum L., Gruppe Phureja und Gruppe Stenotomum, vorhanden. Diese Variation kann in den selbst-verträglichen, 24-chromosomigen Hybriden, die aus der Kreuzung dieser kultivierten Diploiden mit der Gruppe Tuberosum haploid US-W 4 stammen, ausgenützt werden. Nachkommenschaften geselbsteter 24-chromosomiger Hybrid-Klone wurden auf Aufspaltung der früher beschriebenen MarkierungsgeneP, R, Ac, B, I undF und potentiell wertvoller, neuer Markierungsgene untersucht. Die Bonitierung wurde sowohl visuell als auch durch Dünnschicht-Chromatographie der Blüten- und Knollenschalenpigmente vorgenommen. Es wurde ein neues Merkmal gefunden, das die Verteilung von Pigmenten auf der Fruchtknotenwand kontrolliert. Diese Eigenschaft scheint monogenetisch dominant vererbt zu werden, und es wurde ihr das Gen-SymbolOw gegeben. Im Pflanzenmaterial, das für diese Arbeit verwendet wurde, warOw mitI in der Kopplungsphase verbunden und mitF in der Repulsionsphase (Tabellen 1 und 2). Ein die Knollenform kontrollierendes Hauptgen scheint ebenfalls mit dieser Gruppe verbunden zu sein (Tabelle 3). Ein chemogenetischer Markierer wurde in Chromatogrammen entdeckt. Unter ultraviolettem Licht erscheint er als ein glänzendes, fluoreszierendes, blaues Band, im Aussehen sehr ähnlich der Chlorogensäure, aber mit einem niedrigeren Rf-Wert bei gleichem Laufmittel. Dieses Band scheint durch ein einzelnes dominantes Gen vererbt zu sein (Tabelle 4). Auf Grund der aus der visuellen Beurteilung und der Laboruntersuchung der Nachkommenschaften erhaltenen Information wurden die Genotypen von 31 selbstverträglichen Klonen für einige einfach vererbte Merkmale bestimmt. Zum Beispiel konnte nach der Analyse der durch Selbstung des Klons US-W 8775-34 erhaltenen Nachkommenschaft der Genotyppp. RR. FF. ii. owow. Acac bestimmt werden. Klone, wie dieser, mit bekannten Genotypen für einzelne Markierungsgene haben grossen potentiellen Wert für zukünftige genetische Untersuchungen an Kartoffeln.
    Abstract: Résumé La rareté de marqueurs génétiques appropriés, bien définis, a handicapé les études génétiques chez la pomme de terre. Il existe une abondante variation génétique, potentiellement utilisable dans les diploïdes cultivés auto-incompatibles,Solanum tuberosum L. groupe Phureja et groupe Stenotomum. Cette variation peut être exploitée dans les hybrides auto-compatibles à 24 chromosomes dérivés de croisements de ces diploïdes cultivés avec le haploïde US-W4 du groupe Tuberosum. On a retenu les descendances issues de l'autofécondation de clones hybrides à 24 chromosomes pour la ségrégation de gènes marqueurs précédemment décrits,P, R, Ac, B, I etF et de nouveaux gènes potentiellement utilisables. Le classement a été fait à la fois d'après examen visuel et d'après l'analyse chromatographique en couche mince de pigments de fleur et de la peau du tubercule. On a découvert un caractère nouveau qui règle l'apparition des pigments sur la paroi ovarienne. Ce caractère paraît être hérité comme un simple gène dominant, et on lui a attribué le symboleOw. Dans le matériel ‘plante’ utilisé dans la présente étudeOw est lié àI dans la phase d'accouplement et avecF dans la phase de séparation (Tableaux 1 et 2). Un gène majeur qui règle la forme du tubercule paraît également associé avec de groupe de liaison (linkage) (Tableau 3). On détecte un marqueur chimiogénétique dans les chromatogrammes. Sous lumière ultraviolette, il apparaît comme une bande d'un bleu brillant fluorescent très sensible en apparence à l'acide chlorogénique, mais avec une valeur inférieure Rf dans le même solvant de développement. L'hérédité de cette bande est semblable à celle d'un simple gène dominant (tableau 4). On a déterminé les génotypes de 31 clones auto-compatibles pour plusieurs caractères à hérédité simple, en se basant sur le comptage visuel et l'examen au laboratoire des descandances. Par exemple, d'après l'analyse de la descendance d'autofécondation du clone US-W 8775-43, on peut attribuer à celui-ci le génotypepp. RR. FF. ii. owow. Acac. Des clones tels que celui-ci, de génotypes connus pour plusieurs gènes marqueurs, possèdent une grande valeur potentielle pour les futures études génétiques chez la pomme de terre.
    Notes: Summary Selfing 24-chromosome hybrids derived by crossing groups Phureja and Stenotomum with the group Tuberosum haploid US-W 4 has produced progenies segregating for many traits. Monogenic segregation ratios of previously described marker genes were confirmed and the inheritance of two new trats was determined. The previously reported linkage between factors for tuber shape and tuber pigmentation was confirmed. The genotypes for several marker genes have been established on the basis of progeny tests for 31 self-compatible diploid clones. These cultivated diploid stocks with known genotypes for marker genes have great potential value for future genetic investigations in potatoes.
    Type of Medium: Electronic Resource
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