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  • 1
    Electronic Resource
    Electronic Resource
    An evaluation of open and closed systems for in vitro protein digestion of fish meal (1997)
    Oxford, UK : Blackwell Science Ltd
    Aquaculture nutrition 3 (1997), S. 0 
    Details
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The availability of a rapid, cost-effective, accurate and reliable method of assessing fish meal protein digestibility would greatly enhance and standardize quality-control procedures in both the fish meal and fish feed industries. However, while several in vitro digestibility tests have been developed, few have been adopted by industry due to their time-consuming nature, problems surrounding reliability and/or inconsistencies in predictive ability. The present investigation was undertaken to evaluate the utility and predictive qualities of two in vitro digestion assays of distinct design. Methods examined included a novel open system, wherein on-line removal of digestion products was attainable, and a closed system, which permitted analysis of products following completion of the digestion process. Results provided by the two systems were compared using four differentially processed fish meals. The open system supplied information based upon the detected quantity of products below 10 kDa. The closed system provided measurement of free amino groups. Both methods were in agreement with respect to assessing the presence of hydrolysed product. Both systems furnished complementary data with respect to the characterization of fish meal protein quality. The closed and open systems provided insight upon digestibility and digestion kinetic profiles respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2095.1997.00082.x
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  • 2
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary crystallographic analysis of two β-mannanase isoforms from Thermomonospora fusca KW3 (1996)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 1224-1225 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Three β-mannanase isoforms were isolated from the supernatant of a thermophilic actinomycete culture from Thermomonospora fusca KW3. Two of the isoforms (Q1, Q 1.1) were crystallized by the hanging-drop method at room temperature using ammonium sulfate as a precipitant. The isoforms form rod-shaped colorless crystals. Both belong to the orthorhombic space group P212121. The cell dimensions are a = 46.7, b = 61.1, and c = 128.2 Å for isoform Q1, and a = 43.8, b = 46.2, and c = 132.8 Å for isoform Q1.1. The asymmetric unit of either isoform contains one mannanase molecule. Native data have been collected to 2.2 Å resolution for Q1 and to 1.65 Å resolution for Q1.1 using synchrotron radiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444996009006
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  • 3
    Electronic Resource
    Electronic Resource
    Xylanolytic enzyme activities produced by mesophilic and thermophilic actinomycetes grown on graminaceous xylan and lignocellulose (1988)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 55 (1988), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Mesophilic and thermophilic strains of actinomycetes were grown on media containing graminaceous xylan or lignocellulose. Aliquots of the culture fluids were sampled and assayed for enzyme activities involved in the degradation of hemicellulose. Xylanase, acetyl esterase and α-arabinofuranosidase activities could be detected after different times of incubation; their production was also dependent on the growth medium. The highest levels of xylanase activity were found in cultures of strains of Streptomyces, Actinomadura sp. and Saccharomonospora viridis. Streptomyces cyaneus produced the highest amount of arabinofuranosidase whereas acetyl esterase activities were highest in S. cyaneus, S. viridis and Pseudonocardia thermophila.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb13930.x
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  • 4
    Electronic Resource
    Electronic Resource
    Measurements of Tau-Lepton production and decay (1985)
    Springer
    The European physical journal 28 (1985), S. 1-8 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We have studied 419 τ pair events produced in the reactione + e −→τ+ τ− at a c.m. energy of 34.6 GeV. We measure the cross section and angular distribution, as well as the decay branching ratios. The production characteristics are consistent with the Standard Electroweak Model predictions of γ andZ 0 interference. The branching ratios are generally consistent with the τ decaying according to standard weak interaction principles, but we observe somewhat more decays resulting in single charged hadrons plus neutrals than are predicted by present theory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01550242
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  • 5
    Electronic Resource
    Electronic Resource
    A study of energy-energy correlations ine + e − annihilations at $$\sqrt s = 34.6$$ GeV (1985)
    Springer
    The European physical journal 28 (1985), S. 365-370 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We present high statistics measurements of the energy-energy correlation (EEC) and its related asymmetry (AEEC) ine + e − annihilation at a c.m. energy of 34.6 GeV. We find that the energy dependence as well as the large angle behaviour of the latter are well described by perturbative QCD calculations toOα( s 2 ). Non-perturbative effects are estimated with the help of fragmentation models in which different jet topologies are separated using (ɛ, δ) cuts, and found to be small. The extracted values of $$\Lambda _{\overline {MS} }$$ lie between 100 and 300 MeV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01413599
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  • 6
    Electronic Resource
    Electronic Resource
    Tensor meson excitation in the reaction γγ→K S 0 K S 0 (1988)
    Springer
    The European physical journal 37 (1988), S. 329-335 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In the analysis of the reactione + e −→e + e − K S 0 K s 0 clear evidence for exclusive γγ→f 2 ′ resonance production is observed. The productΓ γγ ·B(f 2 ′ →K $$\bar K$$ ) is measured to be 0.10 −0.03−0.02 +0.04+0.03 keV independent of ana priori assumption on the helicity structure. Our data are consistent with a pure helicity 2 contribution and we derive an upper limit for the ratioΓ γγ (0) /Γ γγ . The absence of events in the mass region around 1.3 GeV clearly proves destructivef 2−a 2 interference and allows to measure the relative phases betweenf 2,a 2 andf 2 ′ . Upper limits on the production of the glueball candidate statesf 2(1720) andX(2230) as well as theK S 0 K S 0 -continuum are given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01578125
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  • 7
    Electronic Resource
    Electronic Resource
    A simple model for the velocity modulation detection technique of molecular ions (1989)
    Springer
    Applied physics 48 (1989), S. 235-242 
    Details
    ISSN: 1432-0649
    Keywords: 35.80.+s ; 33.70.−w
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We present a simple model to describe the line-profile of velocity modulated ions. This model is based on Langevin's theory of the mobility and on the independence of the electric field strength on the discharge current in the ideal positive column. A comparison of time constants responsible for the drift of the ions, the concentration, and the ignition and extinction of the discharge itself is essential for a rough understanding of the velocity modulation. The behaviour of H3O+ ions in an ac glow discharge was examined. Under our experimental conditions the line-profile can be explained by a temporal dependence of the velocity which is close to a square-wave. Due to this particular temporal dependence the amplitude of the signal only depends on the concentration modulation, while the line-shape is a simple difference line-profile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00694351
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  • 8
    Electronic Resource
    Electronic Resource
    The influence of GAP promoter variants on hirudin production, average plasmid copy number and cell growth in Saccharomyces cerevisiae (1990)
    Springer
    Current genetics 18 (1990), S. 97-103 
    Details
    ISSN: 1432-0983
    Keywords: Heterologous gene expression ; Plasmid copy number ; GAP promoter ; Hirudin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The yeast Saccharomyces cerevisiae has been engineered to synthesize and secrete desulfato-hirudin (hirudin), a thrombin inhibitor from the leech Hirudo medicinalis. The synthetic gene coding for hirudin was expressed constitutively under the control of four size-variants of the yeast glyceraldehyde-3-phosphate dehydrogenase promoter (GAP) and cloned into a 2 μ based multicopy yeast vector. The constitutive action of the four promoter variants was confirmed by demonstrating that the expression and secretion of hirudin is growth-related. The different efficiencies of the promoter variants not only affected hirudin expression but also led to changes in several cellular parameters, such as cell growth, average plasmid copy number and plasmid stability. The observed changes show that yeast cells establish a specific equilibrium for each promoter variant. We conclude, that the adjustment of cellular parameters in response to the expression levels of a heterologous protein is regulated by two counteracting selective forces: (1) the need for complementation of the auxotrophic host marker by the plasmid-encoded selection gene which, in the case of dLEU2, requires several plasmid copies; and (2) a selective advantage of cells with a lower copy number enabling them to escape the burden of heterologous protein production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312596
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  • 9
    Electronic Resource
    Electronic Resource
    Neurological disease and encephalitis in cats experimentally infected with Borna disease virus (1997)
    Springer
    Acta neuropathologica 93 (1997), S. 391-401 
    Details
    ISSN: 1432-0533
    Keywords: Key words Borna disease virus ; Cat diseases ; Encephalomyelitis ; Immunopathology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Barrier-bred cats were inoculated intracerebrally with either the rabbit-adapted Borna disease virus (BDV) strain V or a newly isolated feline BDV, obtained from a cat with natural staggering disease (SD). Three out of eight inoculated cats developed neurological signs and non-suppurative encephalitis; all three recovered from the acute stage of disease. Sero-conversion and the development of neutralizing antibodies occurred in all of the virus-inoculated cats. In addition, cats inoculated with feline BDV showed an early peripheral T cell response not present in cats inoculated with BDV strain V, suggesting that the feline virus exerted a more vigorous effect on the immune system. Using immunohistochemistry and a reverse transcriptase-polymerase chain reaction assay, BDV-specific antigen and nucleic acid could be demonstrated in brain samples from each cat with encephalitis, showing that incomplete viral clearance was probably responsible for the maintenance of inflammation. The successful induction of neurological signs and encephalitis in one cat infected with feline BDV, together with the detection of BDV-specific antigen and nucleic acid in the brain, provides strong evidence for the notion that BDV is the etiological agent behind feline SD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050630
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  • 10
    Electronic Resource
    Electronic Resource
    Production of cyclomaltononaose (δ-cyclodextrin) by cyclodextrin glycosyltransferases from Bacillus spp. and bacterial isolates (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 314-317 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The conversion of soluble starch to cyclomaltohexaose (α-CD), cyclomaltoheptaose (β-CD), cyclomaltooctaose (γ-CD) and cyclomaltononaose (δ-CD) by cyclodextrin glycosyltransferases (E.C. 2.4.1.19) from Bacillus spp. and bacterial isolates was studied. The results show that δ-CD was formed by all the enzymes investigated in the range of 5%–11.5% of the total amount of α-, β-, γ-, and δ-CD produced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051297
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