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  • 11
    Electronic Resource
    Electronic Resource
    Both genes for EF-1α in Candida albicans are translated (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 5 (1991), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: In previous work, we showed that Candida albicans has two genes, TEF-1 and TEF-2, which encode identical polypeptides for the highly conserved, essential, protein synthesis factor EF-1α (Breviario et al., 1988). This result prompted questions as to whether C. albicans preferentially uses one of the genes over the other and whether both genes are actually translated into protein. Gene-specific sequence differences in the untranslated portion of each gene made it possible to prepare gene-specific oligonucleotide hybridization probes. Results with the probes showed that the relative steady-state mRNA levels of the two genes were equivalent and that the mRNA for each gene was present in active translation complexes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1991.tb01918.x
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  • 12
    Electronic Resource
    Electronic Resource
    Kinetics of rhodopsin at room temperature measured by picosecond spectroscopy (1977)
    [s.l.] : Nature Publishing Group
    Nature 267 (1977), S. 645-646 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Bovine rhodopsin samples, solubilised in Ammonyx LO detergent, were prepared as described in ref. 10. The optically clear samples contained between 1 and 2X104 M rhodopsin (that is, 4-7 absorbance units per cm at 500 nm) and the absorbance ratios for 400 nm/500 nm were 0.20 ±0.02. For the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/267645a0
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  • 13
    Electronic Resource
    Electronic Resource
    Physical property improvements of a pellicular biocatalyst (1991)
    Springer
    Bioprocess and biosystems engineering 7 (1991), S. 71-75 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The thermal stability of an immobilization technique using a pellicular latex matrix was examined in a packed-bed column reactor. The stability was found to vary with liquid flow rate, the type of latex, temperature of operation, and the amount of yeast cells. Adjusting these parameters and introducing particulate inorganic fillers strengthened the latex matrix and improved the thermal stability. Optimization of this immobilization technique resulted in a procedure that allowed latex polymers to be mechanically stable at temperatures up to 50°C. The biological viability of this improved immobilization scheme was demonstrated through the production of L-aspartic acid by immobilized cells of E. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00383581
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  • 14
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    Room Flexibility and Space Use in a Dormitory (1977)
    Beverly Hills, Calif. : Periodicals Archive Online (PAO)
    Environment and behavior. 9:1 (1977:Mar.) 81 
    Details
    ISSN: 0013-9165
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Psychology
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:f117-1977-009-01-000005
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  • 15
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    Office Noise, Satisfaction, and Performance (1994)
    Beverly Hills, Calif. : Periodicals Archive Online (PAO)
    Environment and behavior. 26:2 (1994:Mar.) 195 
    Details
    ISSN: 0013-9165
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Psychology
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:f117-1994-026-02-000004
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  • 16
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    Claudia Goldin and Hugh Rockoff, eds., Strategic Factors in Nineteenth Century American Economic History: A Volume to Honor Robert W. Fogel (Book Review) (1993)
    Boston : Periodicals Archive Online (PAO)
    Business History Review. 67:1 (1993:Spring) 151 
    Details
    ISSN: 0007-6805
    Topics: History , Economics
    Notes: Book Reviews
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:3071-1993-067-01-000008
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  • 17
    Electronic Resource
    Electronic Resource
    Loss of hilar somatostatin neurons following tetanus toxin-induced seizures (1995)
    Springer
    Acta neuropathologica 89 (1995), S. 425-430 
    Details
    ISSN: 1432-0533
    Keywords: Epilepsy ; Hippocampus ; Rat ; Somatostatin ; Tetanus toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A loss of inhibitory interneurons has been reported in the hippocampus following seizure activity in various animal models of epilepsy and in human epileptic tissue. The question of whether particular populations of inhibitory neurons are similarly affected by the chronic block of inhibition tha tresults after tetanus toxin injections directly into the brain has not previously been addressed. In the present study a unilateral intrahippocampal injection of tetanus toxin into the ventral hippocampus was used to produce a chronic epileptic syndrome characterised by brief seizures that recurred intermittently for 6–8 weeks. The results reveal, for the first time, the morphological changes in somatostatin interneurons following tetanus toxin-induced seizures in the rat. A bilateral short-term increase in immunoreactivity of somatostatin neurons is present 1 week after injection. This is accompanied by an increased intensity of somatostatin-immunoreactive axon terminals in the outer molecular layer of the dentate gyrus, which is more marked on the contralateral side. A chronic and significant loss of somatostatin-immunoreactive neurons was noted in the hilus of the dentate gyrus 2 months later. The significance of the chronic loss of the hilar somatostatin neurons in the control of excitatory activity in the dentate gyrus and whether the acute morphological changes are due to a direct action of the toxin on release mechanisms or as a result of seizure activity are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307647
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  • 18
    Electronic Resource
    Electronic Resource
    Loss of hilar somatostatin neurons following tetanus toxin-induced seizures (1995)
    Springer
    Acta neuropathologica 89 (1995), S. 425-430 
    Details
    ISSN: 1432-0533
    Keywords: Key words Epilepsy ; Hippocampus ; Rat ; Somatostatin ; Tetanus toxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A loss of inhibitory interneurons has been reported in the hippocampus following seizure activity in various animal models of epilepsy and in human epileptic tissue. The question of whether particular populations of inhibitory neurons are similarly affected by the chronic block of inhibition that results after tetanus toxin injections directly into the brain has not previously been addressed. In the present study a unilateral intrahippocampal injection of tetanus toxin into the ventral hippocampus was used to produce a chronic epileptic syndrome characterised by brief seizures that recurred intermittently for 6–8 weeks. The results reveal, for the first time, the morphological changes in somatostatin interneurons following tetanus toxin-induced seizures in the rat. A bilateral short-term increase in immunoreactivity of somatostatin neurons is present 1 week after injection. This is accompanied by an increased intensity of somatostatin-immunoreactive axon terminals in the outer molecular layer of the dentate gyrus, which is more marked on the contralateral side. A chronic and significant loss of somatostatin-immunoreactive neurons was noted in the hilus of the dentate gyrus 2 months later. The significance of the chronic loss of the hilar somatostatin neurons in the control of excitatory activity in the dentate gyrus and whether the acute morphological changes are due to a direct action of the toxin on release mechanisms or as a result of seizure activity are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307647
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  • 19
    Electronic Resource
    Electronic Resource
    The use of sodium sulphide-fixed brain tissue for immunocytochemical staining of activated microglia and reactive astrocytes (1993)
    Springer
    Histochemistry and cell biology 99 (1993), S. 91-94 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The use of sodium sulphide-perfused material for the immunocytochemical demonstration of microglia and astrocytes is described. An intracerebroventricular injection of kainic acid (KA) was used to induce neuronal degeneration and subsequent axonal sprouting in the hippocampus. Animals under deep anaesthesia were killed by perfusion with either 4% paraformaldehyde alone or with 1% sodium sulphide followed by 4% paraformaldehyde solution. Microglial cells were identified with OX-42, a monoclonal antibody towards CR3 complement receptors, and astrocytes with a polyclonal antibody to glial fibrillary acidic protein (GFAP). The present study reveals a marked enhancement in the immunoreactivity of activated microglial cells in sodium sulphide perfused tissues compared to those observed in tissues fixed in paraformaldehyde alone. GFAP immunoreactivity of the astrocytes was not compromised by the use of sodium sulphide. The results clearly show the suitability of sodium sulphide perfused tissues for immunocytochemical procedures and should provide a useful tool for investigation of the role of neuroglial cells in axonal sprouting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268026
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  • 20
    Electronic Resource
    Electronic Resource
    Forage Seed Production. I. Temperate Species. (1999)
    Springer
    Crop science 39 (1999), S. 586-586 
    Details
    ISSN: 1435-0653
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/
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