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  • 1995-1999  (2)
  • 1990-1994  (1)
  • 1
    ISSN: 1573-6849
    Keywords: chromosome ; evolution ; FISH ; intrachromosomal rearrangements ; phylogeny ; subregional chromosome painting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have usedAlu polymerase chain reaction generated probes from rearranged human/rodent somatic cell hybrids for fluorescencein situ hybridization and comparative mapping of some intrachromosomal changes in the karyotypes of great apes (Pan troglodytes, P. paniscus, Gorilla gorilla Pongo pygmaeus), a gibbon (Hylobates lar), and an Old World monkey (Macaca fuscata). Probes containing chromosomes 2 and 18 fragments confirmed inversions already suggested by the banding pattern of great ape homologues. However, a chromosome 3 fragment showed complex rearrangements in the gibbon and macaque karyotype which were previously not well defined from banding. ‘Subchromosomal painting’ will allow the identification of intrachromosomal changes on the basis of DNA homology and provides a powerful method to study karyological and genomic evolution.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-6849
    Keywords: FISH ; sheep ; TCRG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1590-3478
    Keywords: TCD-3D ; stroke ; MCA ; basal artery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Sommario Abbiamo studiato 34 pazienti affetti da stroke ischemico acuto, nel territorio dell'Arteria Cerebrale Media (MCA), utilizzando il Doppler transcranico in 3 dimensioni (TCD-3D). I parametri analizzati sono: velocità media; velocità sistolica e diastolica; gli indici di pulsatilità, di asimmetria interemisferica e di trasmissione della pulsatilità. Dei 34 pazienti: 11 hanno presentato un rallentamento marcato della velocità di flusso della MCA dell'emisfero infartuato, con un indice di asimmetria interemisferico (AI) maggiore del 40%; 8 pazienti un rallentamento modesto del flusso della MCA, con AI compreso tra 25–40%; in 2 pazienti abbiamo evidenziato, indirettamente, circoli collaterali di pertinenza dell'arteria cerebrale anteriore, in associazione ad un rallentamento del flusso della MCA; 5 pazienti mostravano una stenosi dell'MCA. Infine 9 pazienti non hanno presentato alterazioni dei parametri Doppler. La correlazione tra sintomatologia neurologica e AI è risultata significativa (r=0.76). La non invasività, la facile ed immediata esecuzione e soprattutto, la buona attendibilità dell'esame, ci consente di affermare che il TCD-3D, introducendo miglioramenti sostanziali nella metodica del Doppler transcranico tradizionale, risulta particolarmente utile nella valutazione emodinamica della circolazione cerebrale in caso di stroke ischemico.
    Notes: Abstract We studied 34 patients with acute ischeiic stroke in the territory of the middle cerebral artery (MCA) by three-dimensional transcranial Doppler (TCD-3D). The parameters analyzed were: mean blood flow velocity, systolic and diastolic velocities; indices of pulsatility, hemisphere asymmetry and pulsatility transmission. Of the 34 patients 11 presented marked slowing of flow velocity in the MCA on the infarct side with an asymmetry index (AI) of over 40%, 8 patients with slightly reduced flow velocity in the MCA and an AI of 25–40%, 2 patients in whom there was indirect evidence of collateral circulations in the anterior cerebral artery distribution together with slowing of MCA flow; 5 patients had stenosis of the MCA, 9 patients showed no alterations of the Doppler parameters. The correlation between neurological symptom pattern and AI was significant (r=0.76). Noninvasive, easy to perform, performable at once and reliable, TCD-3D is a great improvement on traditional transcranial Doppler and is especially useful in assessing the hemodynamics of the cerebral circulation in ischemic stroke.
    Type of Medium: Electronic Resource
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