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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture nutrition 1 (1995), S. 0 
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Emulsions with different content of (n-3) PUFA and vitamin A were used to enrich Artemia and examine the effect of these nutrients on pigmentation success in turbot, Scophthalmus maximus (L.). The best pigmentation rates were obtained using an overdose of vitamin A (500 000 IU L−1), but coincided with a high incidence of skeletal deformations. Higher growth and pigmentation rates were achieved by increasing the quantity of (n-3) PUFA oil in the emulsion. The use of (n-3) PUFA-deficient diet caused the highest occurrence of albinism as well as a cessation of metamorphosis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture nutrition 3 (1997), S. 0 
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two feeding experiments were conducted to assess the quantitative cholesterol requirement of the juvenile prawn, Penaeus japonicus. The prawns were fed casein-based (experiment 1) and casein- or crab protein-based (experiment 2) diets with or without supplemental cholesterol. The daily increases in quantities of body cholesterol (mg kg−1 prawn day−1) at maximum growth and dietary cholesterol intake over a 40-day feeding period were determined. Prawns fed 5 g kg−1 supplemental cholesterol, regardless of protein sources, gave the highest weight gain. Body retention efficiencies of dietary cholesterol (cholesterol retained × 100/cholesterol intake) vary among prawns fed 5 g kg−1 supplemental cholesterol (diets 3, 5 and 7) but dietary cholesterol requirements expressed as mg kg−1 body weight (BW) day−1 were not significantly different (180 to 200 mg kg−1 BW day−1). Based on dietary cholesterol requirement, the optimum dietary cholesterol levels for the juvenile prawns were estimated in relation to feeding levels. When feeding levels were 3%, 5% and 7% of body weight, optimum dietary cholesterol levels were 5.0 to 6.0, 3.6 to 4.0, and 2.6 to 2.9 g kg−1 of dry diet, respectively. The present study showed the advantages of determining daily cholesterol requirement (mg kg−1 BW day−1) at maximum growth through a factorial method in determining optimum dietary cholesterol levels in P. japonicus.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 117 (1998), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Changes in the composition of mitochondrial DNA (mtDNA) in tobacco during the processes of long-term tissue culture and regeneration were detected by DNA gel blot analyses using several mtDNA fragments as probes. The changes in mtDNA were identified by differential accumulations of mtDNA fragments in cultured cells that were hybridized with probes atp6, cob, and nad3-rpsl2. The changes detected by each probe did not appear to be linked, since they were different with each probe and in different subcultures of calli. Based on the current results and previous detailed analyses of the changes detected by atp6, a possible model that explains the alterations in mtDNA during tissue culture is presented. In this model, the changes are attributed to multiple shifts in the equilibrium that was maintained between different subgenomic mtDNA molecules.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture research 28 (1997), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The dietary effect of arachidonic acid (20:4n-6, AA) on the composition of the head and the body, and on the pigmentation of Japanese flounder, Paralichthys olivaceus (T. & S.), was studied by means of microbound diets. Increasing levels of this essential fatty acid produced higher growth and pigmentation success in juveniles after 45 days of feeding. The role played by AA in the composition of phosphatidylinositol and eicosanoid synthesis is discussed in relation to the normal function of the neural system and the production of hormones involved in the metamorphosis and pigmentation of flatfish.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Aquaculture nutrition 2 (1996), S. 0 
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The valine requirement of juvenile tiger shrimp, Penaeus monodon Fabricius, was determined. Shrimp postlarvae, PL20, with a mean weight of 14 mg, were randomly distributed in 36 oval 40-L capacity fibreglass tanks at 10 shrimp per tank in a flow-through seawater system and reared for 8 weeks. Postlarvae were fed amino acid test diets containing 400 g kg−1 protein with casein and gelatine as intact sources of protein. Crystalline L-amino acids were supplemented to simulate the amino acid profile of the shrimp muscle except valine. Valine was added in graded levels to obtain 7, 10, 13, 16, 19 and 22 g kg−1 of the diet or 18, 25, 33, 40, 48 and 55 g kg−1 of dietary protein. At termination of the feeding experiment, growth and survival were determined and nutritional deficiency signs noted. The relationship between weight gain and dietary valine level was analysed by the broken-line regression method to derive the valine requirement. The dietary valine requirement of Penaeus monodon postlarvae was found to be 13.5 g kg−1 of the diet or 34 g kg−1 of dietary protein. This value was lower than the level found in the shrimp tissue.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1365-2095
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Diets reduced in methionine (Met) and with oxidized oil were formulated to induce visual deficiencies in Japanese flounder, Paralichthys alivaceus (T. & S.), in an attempt to link vision with pigmentation development. Even though Met-deficient diets have proved to be cataractogenic in salmonids, no cataracts were formed in flounder eye. However, both diets produced an abnormal morphology in the retinal pigment epithelium and the photoreceptor outer segments of the fish retina. The high presence of dead nuclei in the photoreceptor inner segment suggests a loss in the visual capability of fish fed these two diets, which produced lower pigmentation success than the control. A significant correlation was also found between pigmentation success and fatty acid composition (docosahexaenoic acid and arachidonic acid) of the head polar lipid fraction. The results suggest that a deficient intake of amino acids and fatty acids produces a change in retinal structure and composition, leading to reduced visual capability and suppression of the development of normal pigmentary pattern in flatfish.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 33 (1998), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study was designed to demonstrate, by use of biotin-labeled hyaluronic acid binding protein (HABP) and an avidin–enzyme system, the localization of hyaluronan (HA) in periodontal tissue of beagle dogs during experimentally induced periodontitis. Experimental periodontitis was induced in the dogs by ligation of the gingival sulcus. Experimental tissue was collected at 0, 3, 7 and 21 days after ligation. HA was revealed by strong staining in the intercellular space around epithelial cells and periodontal ligament, and by light staining in the gingival connective tissue. According to the progression of periodontal tissue breakdown, HA was detected in a small number of leukocytes and monocytes, on the surface of osteoclasts, the surface of alveolar bone, thickened endothelium and in epithelial cells related to rete peg formation. Streptomyces hyaluronidase-treated specimens gave negative staining. This study suggests that HA may be associated with the inflammatory reaction in experimental periodontitis tissue.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 33 (1998), S. 0 
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This study was designed to demonstrate, by use of biotin-labeled hyaluronic acid binding protein (HABP) and an avidin-enzyme system, the localization of hyaluronan (HA) in periodontal tissue of beagle dogs during experimentally induced periodontitis. Experimental periodontitis was induced in the dogs by ligation of the gingival sulcus. Experimental tissue was collected at 0, 3, 7 and 21 days after ligation. HA was revealed by strong staining in the intercellular space around epithelial cells and periodontal ligament, and by light staining in the gingival connective tissue. According to the progression of periodontal tissue breakdown, HA was detected in a small number of leukocytes and monocytes, on the surface of osteoclasts, the surface of alveolar bone, thickened endothelium and in epithelial cells related to rete peg formation. Streptomyces hyaluronidase-treated specimens gave negative staining. This study suggests that HA may be associated with the inflammatory reaction in experimental periodontitis tissue.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1439-0426
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An attempt was made to measure the apparent digestibility (AD) of cholesterol and fatty acids using 5aL-cholestane as a marker (cholestane-method) using tilapia. Oreochromis niloticus, and the freshwater prawn, Macrobrachium rosenbergii, as test animals. The results were compared with those obtained by the Cr2O3-method. Casein-based test diets containing 0.3% levels of cholestane and chromic oxide as markers were prepared for determination of AD of dietary cholesterol and fatty acids. After 2 weeks of acclimation, the test animals, tilapia (average 3.0 g wet weight) and freshwater prawn (average 0.5 g wet weight) were fed the test diets for 1 week, and faecal samples were collected over 6 subsequent days. Lipids in the diets and faeces were extracted with chloroform-methanol and saponified with 10% KOH in methanol. Gas-liquid chromatography (GLC) on 1.5% OV-17 of the unsaponifiable matters afforded the quantities of 5aL-cholestane and cholesterol, whereas that on 5% Shinchrom E-71 of the saponifiable matters provided the quantities of fatty acids. Chromic oxide was determined by a wet-digestion method. In tilapia, the AD of cholesterol determined by cholestane- and Cr2O3-methods were 78.1% and 73.5%, respectively. The AD of individual fatty acids differed with the types of fatty acids, but those of fatty acids determined by the two methods in this study were similar, except for a slightly higher value of several fatty acids such as 16:1 in the cholestane-method than in the Cr2O3-method. This indicates that 5α-cholestane as well as Cr2O3 can be conventionally used as a marker for determining AD of fatty acids and cholesterol. The cholestane-method is advantageous as it permits parallel analysis of cholesterol (or fatty acids) and 5α-cholestane by GLC using small amounts of faecal sample. The cholestane-method was also useful for analysing digestibilities of cholesterol and total lipids in freshwater prawn. However, slight differences between the cholestane- and Cr2O3-methods were observed in the AD of several fatty acids such as 14:0 and 16:1.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Marine biology 103 (1989), S. 169-173 
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The performance of an artificial practical diet, kappacarrageenan microbound diet (C-MBD) was assessed on Penaeus monodon larvae at the SEAFDEC Broodstock and Maturation Experimental Laboratory in March 1986. Shrimps were reared from zoea1 to post-larvae1 using five dietary treatments: (a) natural food — Chaetoceros calicitrans and Artemia salina (b) C-MBD; (c) combination of natural food and C-MBD; (d) commercial diet (microencapsulated, MED); (e) combination of natural food and commercial diet. Results showed slow development with larvae fed the commercial diet. Feeding with C-MBD in combination with natural food resulted in the highest % survival among treatments (69.6), but this was not significantly different (P〉0.05) from those obtained with larvae fed natural food alone, C-MBD alone or their combination. While mean values for survival of larvae fed the commercial diet, either alone or in combination, was significantly lower (p〈0.05) than all other treatments, their mean growth indices were comparable with larvae fed C-MBD alone or in combination. The low levels of protein, lipid and essentially fatty acids (which are considered important nutrients during larval development) contained in the commercial diet may well justify the results on metamorphosis, survival and growth of the larvae fed this diet. The good performance of C-MBD in this experiment suggests that this kind of diet can be used as partial or total replacement to the traditional algal food.
    Type of Medium: Electronic Resource
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