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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of Deoxyshikonin, an Antidermatophytic Principle from Lithospermum erythrorhizon Cell Cultures (1988)
    s.l. : American Chemical Society
    Journal of natural products 51 (1988), S. 152-154 
    Details
    ISSN: 1520-6025
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/np50055a025
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Circadian rhythms of demand-feeding and locomotor activity in rainbow trout (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 52 (1998), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Under free-running conditions, most rainbow trout displayed circadian feeding rhythms, although the expression of circadian rhythmicity depended on the experimental condition: 16·7% of fish under constant dim light (LL dim), 66·1% under a 45 :45 min light-dark cycle (LD pulses), and 83·8% under constant light (LL). Under LD pulses, the period length of the free-running rhythms for feeding was significantly shorter (21·9 ± 0·7 h, n=8) than under LL (26·2 ± 0·3 h, n=10). Period length for locomotor activity under LL was 25·8 ± 0·6 h (n=4). Under LD conditions, the daily demand-feeding profile was always confined to the light phase and chiefly composed of two main episodes, directly after lights on (light elicited) and in anticipation to lights off (endogenous). Contrasting to feeding, the diel locomotor activity profile varied remarkably: a diurnal activity pattern at the bottom, while a clearly nocturnal pattern at the surface. These results contribute to a better understanding of feeding and locomotor rhythms of rainbow trout, providing evidence for the existence of a biological clock involved in their circadian control. This finding contrasts with the previously recorded lack of an endogenous oscillator in the pineal organ driving the rhythmic secretion of melatonin, which suggests different locations from the pineal for the circadian pacemakers in this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1998.tb00797.x
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  • 3
    Electronic Resource
    Electronic Resource
    Feeding entrainment of locomotor activity rhythms in the goldfish is mediated by a feeding-entrainable circadian oscillator (1997)
    Springer
    Journal of comparative physiology 181 (1997), S. 121-132 
    Details
    ISSN: 1432-1351
    Keywords: Key words Locomotor activity ; Feeding-entrainment ; Circadian rhythms ; Vertical distribution ; Goldfish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Periodic food availability can act as a potent zeitgeber capable of synchronizing many biological rhythms in fishes, including locomotor activity rhythms. In the present paper we investigated entrainment of locomotor rhythms to scheduled feeding under different light and feeding regimes. In experiment 1, fish were exposed to a 12:12 h light/dark cycle and fed one single daily meal in the middle of the light phase. In experiment 2, we tested the effect of random versus scheduled feeding on the daily distribution of activity. During random feeding, meals were randomly scheduled with intervals ranging from 12 to 36 h, while scheduled feeding consisted of one single daily meal set in the middle of the light or dark phase. Finally, in experiment 3, we studied the synchronization of activity rhythms to feeding under constant darkness (DD) and after shifting the feeding cycle by either advancing or delaying the feeding cycle by 9 h. The results revealed that goldfish synchronized to feeding, overcame light entrainment and significantly changed their daily distribution of activity according to their feeding schedule. In addition, the daily activity pattern modulated by feeding differed between layers: a peak of activity being noticeable directly after feeding at the bottom, while an anticipatory behaviour was obvious at the surface of the tank. Under DD and no food, free-running rhythms averaging 25.5 ± 1.9 h (mean ± SD) were detected. In conclusion, some properties of feeding entrainment (e.g. anticipation of the feeding time, free-running rhythms following termination of periodic feeding, and the stability of ø after shifting the feeding cycle) suggested that goldfish have (a) separate but tightly coupled light- and food-entrainable oscillators, or (b) a single oscillator that is entrainable by both light and food (one synchronizer being eventually stronger than the other).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003590050099
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  • 4
    Electronic Resource
    Electronic Resource
    Positron Trapping Defects in Neutron-Irradiated Vitreous and Crystalline SiO〈sub〉2〈/sub〉 (1997)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 255-257 (Sept. 1997), p. 460-462 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/02/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.255-257.460.pdf
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  • 5
    Electronic Resource
    Electronic Resource
    Regulation of shikonin production by glutamine in Lithospermum erythrorhizon cell cultures (1987)
    Springer
    Plant cell reports 6 (1987), S. 131-134 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Amino acid analysis has shown that Lithosperum erythrorhizon cell suspension cultures which are unable to produce shikonin derivatives in LS medium containing ammonium accumulate a large quantity of glutamine, as compared with shikonin-producing cells cultured in the production medium M9 containing nitrate as the sole nitrogen source. The addition of glutamine to M9 medium proved to be strongly inhibitory to shikonin production. Furthermore, culture experiments using an inhibitor of glutaminase suggested that shikonin synthesis is not inhibited by ammonium released from glutamine but by glutamine itself. These findings indicate that the repression of shikonin synthesis occurs in close association with an accumulation of glutamine in cultured cells grown in a medium containing ammonium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276670
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  • 6
    Electronic Resource
    Electronic Resource
    Cytological changes associated with induction of anthraquinone synthesis in photoautotrophic cell suspension cultures of Morinda lucida (1987)
    Springer
    Plant cell reports 6 (1987), S. 187-190 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differences in subcellular structures between anthraquinone-producing and non-producing cells were investigated using photoautotrophic and photoheterotrophic cell suspension cultures of Morinda lucida. Irregular or distorted plastids containing starch grains were observed in the anthraquinone-producing cells, together with a highly elongated rough endoplasmic reticulum. The possible participation of plastids and rough endoplasmic reticulum in the anthraquinone biosynthesis is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268475
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  • 7
    Electronic Resource
    Electronic Resource
    An immobilized cell culture system for berberine production by Thalictrum minus cells (1987)
    Springer
    Plant cell reports 6 (1987), S. 185-186 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thalictrum minus cells immobilized in calcium-alginate beads were cultured in a newly devised bioreactor for the purpose of producing berberine. This system could prevent cells from leaking out of the beads, allowing them to release most of the berberine synthesized into the liquid medium. Furthermore, the biosynthetic capability of the immobilized cells was found to be comparable to that of freely suspended cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268474
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  • 8
    Electronic Resource
    Electronic Resource
    Correlation of papain-like enzyme production with laticifer formation in somatic embryos of papaya (1989)
    Springer
    Plant cell reports 8 (1989), S. 251-254 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A protease similar to papain was produced by somatic embryos ofCarica papaya in association with the development of laticifers containing characteristic vesicles which probably originated from the endoplasmic reticulum. In contrast to somatic embryos, a papain-like protease was not detected in either friable or compact callus cultures which failed to develop laticifers. These observations strongly suggest that the differentiation into laticifers is required for papain production in papaya.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00778545
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of oxygen supply on berberine production in cell suspension cultures and immobilized cells ofThalictrum minus (1989)
    Springer
    Plant cell reports 8 (1989), S. 255-258 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ample supply of O2 proved to be of great importance for berberine production in cell suspension culture ofThalictrum minus, as the specific O2 consumption rate of berberine-producing cells was twice as high as that of non-producing cells. Furthermore, berberine yield increased with increases in the volumetric O2 transfer coefficient (KLa). Estimation of the optimum conditions of oxygen supply in suspension cultures and immobilized cells according to a known theoretical model assuming O2 uptake by cells to be a zero-order reaction was in good agreement with the experimental data. The O2 supply to immobilized cells could be improved by reducing the cell density and radius of the bead.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00778546
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  • 10
    Electronic Resource
    Electronic Resource
    Cytological changes associated with alkaloid production in cultured cells of Coptis japonica and Thalictrum minus (1986)
    Springer
    Plant cell reports 5 (1986), S. 65-68 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell structures were compared between alkaloid-producing and non-producing cell cultures of Coptis japonica and Thalictrum minus by electron microscopic observation. In alkaloid-producing cells of C. japonica, prior to the onset of alkaloid synthesis, the vacuoles showed a greater volume than in non-producing cells. These were characterized by a number of large starch grains in the cytoplasm. Furthermore, alkaloid-producing cells contained stacks of rough endoplasmic reticulum. Comparison of different cell lines suggested that there might be a negative correlation between accumulation of alkaloids and starch. Similar cytological differences were observed with T. minus cell cultures that release berberine into the culture medium. Alkaloid producing cells were found to contain an abundance of cytoplasmic vesicles (0.5 – 1 υm in diameter).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269721
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