ISSN:
1432-072X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The organism grown on Δ1-p-menthene was found to grow without any lag on methyl isopropyl ketone, isobutyrate, succinate, malate, lactate and acetate. Isobutyrate or acetate grown cells grew on Δ1-p-menthene after a lag and showed comparatively little growth on β-isopropyl pimelic acid. Δ1-p-menthene grown cells oxidized readily isobutyrate, acetate, succinate, malate, α-ketoglutarate and methacrylate. Methylmalonate, methyl isopropyl ketone and β-isopropyl pimelic acid were rather oxidized at slow rates. Isobutyrate grown cells, on the other hand, showed from very good to very fair oxidation rates with succinate, isobutyrate, acetate, malate, methacrylate, α-ketoglutarate. Methylmalonate was oxidized much better and methyl isopropyl ketone was oxidized slowly. Δ1-p-menthene and isobutyrate grown cells were used under resting conditions with different substrates in the presence of arsenite. Analysis of the reaction products indicated the accumulation of a keto acid. Qualitative analysis of the keto acid formed by TLC showed pyruvate as the major ketocarboxylic acid with one or two other minor components. The major component had been isolated and identified as pyruvic acid. Similar results had been obtained by working with crude cell-free enzyme preparations. Based on these results two possible mechanisms of degradation of isobutyrate have been suggested. A plausible pathway has been outlined for the terminal oxidation pattern in the Pseudomonad (PL-strain).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00410159
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