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  • 1995-1999  (2)
  • 1980-1984  (1)
  • 1955-1959
  • 1880-1889
  • Endothelial cells  (2)
  • Diagnostic  (1)
  • 1
    ISSN: 1432-1912
    Keywords: Key words 5-Hydroxytryptamine (5-HT ; serotonin) ; 5-HT1D receptors ; 5-HT1Dβ receptors ; Endothelial cells ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pharmacological evidence has suggested the presence of 5-hydroxytryptamine (5-HT, serotonin), 5-HT1D receptors on endothelial cells but these receptors have never been identified unambiguously on this type of cells. We now report that human umbilical vein endothelial cells (HUVEC) express 5-HT1D receptors coupled to inhibition of cyclic AMP formation. 5-HT and the 5-HT1D receptor agonists 5-carboxamidotryptamine (5-CT) and sumatriptan were approximately equipotent at inhibiting forskolin-stimulated cyclic AMP accumulation in HUVEC (mean pEC50 7.6–8.2, maximal effect 30% inhibition). The 5-HT1A receptor agonist, 8-OH-DPAT was clearly less potent (pEC50 6.2) and less efficacious. The selective 5-HT1D receptor antagonist, GR127935 (1 nM) markedly inhibited the effect of 5-HT (apparent pKB 10.8). Reverse transcription-polymerase chain reaction analysis showed the mRNA for 5-HT1Dβ receptors to be expressed in HUVEC. These results demonstrate the presence of functional 5-HT1D receptors and the expression of 5-HT1Dβ receptor mRNA in HUVEC. They support the involvement of 5-HT1Dβ receptors in endothelial-mediated responses to 5-HT.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1912
    Keywords: 5-Hydroxytryptamine (5-HT, serotonin) ; 5-HT1D receptors ; 5-HT1Dβ receptors ; Endothelial cells ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pharmacological evidence has suggested the presence of 5-hydroxytryptamine (5-HT, serotonin), 5-HT1D receptors on endothelial cells but these receptors have never been identified unamiguously on this type of cells. We now report that human umbilical vein endothelial cells (HUVEC) express 5-HT1D receptors coupled to inhibitiion of cyclic AMP formatin. 5-HT and the 5-HT1D receptor agaonists 5-carboxamidotryptamine (5-CT) and sumatriptan were approximately equipotent at inhibiting forskolin-stimulated cyclic AMP accumulation in HUVEC (mean pEC50 7.6–8.2, maximal effect 30% inhibition). The 5-HT1A receptor agonist, 8-OH-DPAT was clearly less potent (pEC50 6.2) and less efficacious. The selective 5-HT1D receptor antagonist, GR127935 (1 nM) markedly inhibited the effct pf 5-HT (apparent pKB 10.8). Reverse transcription-polymerase chain reaction analysis showed the mRNA for 5-HT1Dβ receptors to be expressed in HUVEC. These results demonstrate the presence of functional 5-HT1D receptors and the expression of 5-HT1Dβreceptor mRNA in HUVEC. They support he involvement of 5-HT1Dβ receptors in endothelial-mediated responses to 5-HT.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 352 (1980), S. 522-522 
    ISSN: 1435-2451
    Keywords: Recurrent ulcer ; Diagnostic ; Individual surgical treatment ; Rezidivulcus ; Funktionelle Diagnostik ; Individualisierende Operation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Auswahl des Operationsverfahrens im Korrektureingriff wegen eines Rezidivulcus sollte der vielschichtigen Pathogenese gerecht werden. Die Indikation zur konservativen Behandlung oder operativer Korrektur (Vagotomie oder Resektion alleine oder kombiniert) hängt wesentlich ab vom Ausfall präoperativer Funktionsteste, welche Röntgenuntersuchung, Sekretionsanalyse, Gastrinprofil nach Provokation und nuklearmedizinische Messungen von Magenentleerung und Gallereflux einschließen. Die guten Erfahrungen mit einem abgestuften Indikationsschema werden an 135 Fällen (94 Upj, 41 Rezidiven nach Vagotomie) dargestellt.
    Notes: Summary The choice of treatment (medical or surgical with single vagotomy or resection or a combination of the two methods) in recurrent ulceration after resection or nonresection depends on the results of different functional studies before reoperation. They include X-ray examination, secretion analysis, determination of serum gastrin in provocation and feeding tests, and measurement of gastric emptying and jejuno- or duodenogastric reflux by scintiscan. Good results are reported in 135 cases with the use of different procedures based on functional diagnostic parameters.
    Type of Medium: Electronic Resource
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