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  • 1995-1999  (1,427)
  • 1975-1979  (821)
  • 1955-1959  (396)
  • 1830-1839  (10)
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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 17 (1978), S. 1828-1833 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Biochemistry 17 (1978), S. 1822-1827 
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 13 (1999), S. 0 
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Tumour necrosis factor-alpha (TNFα) has been suspected of playing an important role in the pathogenesis of inflammatory bowel diseases, and has become a target for the treatment of these diseases. Open-label, placebo controlled studies have shown that engineered CDP571 and chimeric anti-TNF antibody (cA2) provide a significant benefit in Crohn’s disease. Since these antibodies have to be used repeatedly to maintain remission in inflammatory bowel disease, there is a concern that their use may compromise host defence and produce toxic side-effects.〈section xml:id="abs1-2"〉〈title type="main"〉Methods:We evaluated the combined use of mouse specific TNFα mab (25 μg/mouse, Endogen) and pentoxifylline (PF, 100 mg/kg/day, p.o., TNFα release inhibitor) in the DSS (3% dextran sulphate solution) model of mouse colitis. Colitis was induced by the feeding of 3% DSS for three cycles. The study groups were: Group I: single injection of rat anti-mouse IgG, Group II: single injection of TNFα mab, Group III: daily PF for three cycles, Group IV: single injection of TNFα mab + PF for three cycles, Group V: TNFα mab at the beginning of each cycle (three injections) and Group VI: TNFα mab (three injections) + daily PF for three cycles. Daily disease activity (DAI) was measured throughout the study. At the end of each cycle, colon tissue was processed for histology, myeloperoxidase (MPO) and plasma TNFα.〈section xml:id="abs1-3"〉〈title type="main"〉Results:Mice treated with a single injection of TNFα alone or TNFα mab + PF showed significantly lower DAI, inflammation scores and ulcer index compared with the IgG treated group. Mice treated with TNFα mab + PF had no ulcers. Multiple injections of TNFα mab or TNFα mab + PF showed greater inhibition in DAI and cytokines in the first two cycles. However, in the third cycle, multiple injections of TNFα mab showed adverse proinflammatory effects.〈section xml:id="abs1-4"〉〈title type="main"〉Conclusion:The simultaneous administration of pentoxifylline and TNFα mab may enhance therapeutic outcomes in inflammatory bowel disease and reduce the side-effects associated with the repeated use of TNFα mab.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 43 (1996), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . In vitro excystation of Spironucleus muris cysts, purified by sequential sucrose and Percoll gradients from mouse feces, was studied. Three in vitro excystation procedures, used for Giardia, were assessed to determine the most useful method. Excystation was monitored by light microscopy and subsequently characterized by transmission and scanning electron microscopy. Spironucleus muris excysted routinely at a level greater than 90% when induced in Hanks’ balanced salt solution containing sodium bicarbonate at pH 2.0 and transferred to Tyrodes’ salt solution as an excystation medium. Similarly, high rates of excystation were recorded after induction of S. muris cysts in 0.1 M potassium phosphate buffer (pH 7.0) with sodium bicarbonate and excystation in trypticase-yeast extract-iron medium (TYI medium) or phosphate-buffered saline. A lower rate and percentage of excystation were observed after induction of S. muris cysts in an aqueous hydrochloric acid solution (pH 2.0) followed by excystation in TYI medium. All excystation methods produced extremely active S. muris trophozoites with normal morphology. Nonexcysting S. muris cysts have a wall composed of an outer fibrous and an inner membranous portion. Following induction, numerous vesicles appeared in the peritrophic space. Excystation began by the cyst wall opening at one pole, and the anterior part of the trophozoite protruding from the cyst wall. The trophozoite emerged progressively from the cyst wall and the empty cyst wall appeared to collapse. Excysted trophozoites exhibited normal morphological features of S. muris trophozoites isolated from the mouse intestine.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1433-0423
    Keywords: Schlüsselwörter Pseudophakie ; Keratoplastik ; Sekundäre bullöse Keratopathie ; Fuchs-Dystrophie ; Hinterkammerlinse ; Key words Pseudophakic keratoplasty ; Secondary bullous keratopathy ; Fuchs' dystrophy ; Posterior chamber lens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Penetrating keratoplasties in pseudophakia were added to a group with limited prognosis. This was especially valuable, if iris-supported and anterior chamber intraocular lenses were implanted. Since today mainly posterior chamber lenses are implanted, the question of the long-term prognosis in this type of lens is important. Patients and method: From 1985 to 1994, 62 penetrating keratoplasties in pseudophakic eyes with posterior chamber IOL were performed: group I: decompensated Fuchs dystrophy (26 patients), group II: secondary surgery-related bullous keratopathy (36 patients) exclusively. Minimal follow-up was 18 months, mean age was 73.7 years. Results: The mean follow-up period was 32 months. The mean visual acuity on delivery was 0.11. After 6 months it was 0.23, after 1 year 0.3, after 2 years 0.35 and after 3 years 0.41 without significant differences in either group. Two years after transplantation 52.8% gained a visual acuity (VA) of ≥0.3, after 3 years 58.6% of which 41.1% had a VA of ≥0,5. There was a visual improvement in 83.4%; the mean spherical equivalent was –0.29 D. Refractive errors within 2 D of emmetropia were found in 56.7%; 95.1% of the grafts remained clear. Five patients had a reversible graft rejection. Extracorneal factors of impaired vision were observed in 25 patients. Conclusion: Despite a different VA before surgery, the visual outcome in the two groups was identical. Penetrating keratoplasty in Fuchs dystrophy and secondary bullous keratopathy with posterior chamber IOL have a much better long-term prognosis than those with iris-supported and anterior chamber IOL. Postoperative complications and graft rejections are rare.
    Notes: Perforierende Keratoplastiken bei Pseudophakie zählten bisher zur Gruppe mit einer eingeschränkten Prognose. Dies galt insbesondere, wenn iris- und kammerwinkelgestützte Intraokularlinsen implantiert wurden. Es ergibt sich die Frage wie die Langzeitprognose bei der üblichen Hinterkammerlinsenimplantation ist. Patienten und Methode: Im Zeitraum von 1985 – 1994 wurden 62 perforierende Keratoplastiken an pseudophaken Augen mit Hinterkammerlinse durchgeführt wegen: Gruppe I: dekompensierte Fuchs-Dystrophie (26 Patienten), Gruppe II: sekundär operationsbedingte bullöse Keratopathie (36 Patienten). Die Mindestnachbeobachtung betrug 18 Monate. Das mittlere Alter lag bei 73,7 Jahren. Ergebnisse: Bei einer durchschnittlichen Nachbeobachtung von 32 Monaten betrug das mittlere Sehvermögen bei der Entlassung 0,11, nach 6 Monaten 0,23, nach 1 Jahr 0,3, nach 2 Jahren 0,35, nach 3 Jahren 0,41 ohne signifikanten Unterschied in beiden Gruppen. Zwei Jahre nach der Transplantation hatten 52,8% der Patienten einen Visus von ≥0,3, nach 3 Jahren waren es 58,6%, davon 41,1% mit einem Visus ≥0,5, das entspricht einer Visusverbesserung bei 83,4%. Das durchschnittliche sphärische Äquivalent betrug –0,29 dpt. 56,7% lagen zwischen + und –2 dpt. 95,1% der Transplantate blieben klar. Bei 5 Patienten kam es zu einer reversiblen Abstoßungsreaktion. Extrakorneale Ursachen einer Visusminderung wurden bei 25 Patienten beobachtet. Schlußfolgerung: Trotz unterschiedlichem Ausgangsvisus waren die postoperativen Ergebnisse in beiden Gruppen gleich. Perforierende Keratoplastiken wegen Fuchs-Dystrophie und sekundär bullöser Keratopathie haben bei HKL eine wesentlich bessere Langzeitprognose als bei iris- und kammerwinkelgestützten IOL. Postoperative Komplikationen und Transplantateintrübungen sind selten.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Calcified tissue international 27 (1979), S. 19-26 
    ISSN: 1432-0827
    Keywords: Bone diseases ; Familial hypophosphatemia ; Magnesium ; Mice ; Phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary A new genetic mutant in mice,Hyp, has been proposed as a model for the human disease X-linked hypophosphatemia (the most common form of vitamin D-resistant rickets). The gene is X-linked, dominant, and produces reduced renal tubular reabsorption of phosphate, hypophosphatemia, and dwarfism. Our goal was to evaluate the skeletal changes histologically and to measure chemically the prominant blood and bone minerals to judge the suitability of this mutant as a model for the human disease. Thirteen-week-old hemizygousHyp male mice were compared with their normal littermate controls. TheHyp mice were hypocalcemic, hypophosphatemic, hypermagnesemic, and had elevated plasma alkaline phosphatase. The femur ash weighed less than half the normal ash weight but had a normal Ca:P ratio. The ash composition was high in %Na and K but low in %Mg. The mandibular incisor ash was also low in %Mg. Histologically the femur showed wide osteoid borders and wide epiphyseal plate. Microradiography revealed reduced bone density and enlarged osteocyte lacunae. Skeletal muscle samples, although smaller in theHyp mice, showed no striking alternations in inorganic or total phosphate content, dry weight (as % wet weight), or extracellular fluid space. TheHyp gene in mice seems to produce a condition similar to that of X-linked hypophosphatemia in humans.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 31 (1958), S. 179-190 
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Zusammenfassung Es wird über Versuche berichtet, in denen Sporen von Oospora lactis einzeln mit ultraviolettem Licht von der Wellenlänge 257 mμ bestrahlt und auch einzeln in ihrem weiteren Schicksal verfolgt wurden. Die Dosis wurde so gewählt, daß etwa die Hälfte der 90–100 bestrahlten Individuen überlebte, die andere Hälfte letal geschädigt wurde. Ein kleiner Anteil der so bestrahlten Individuen zeigte Wachstumsanomalien, die längere Zeit, auch über mehrere Einzell-Subkulturen hinweg, erhalten blieben, aber schließlich auf normale Formen hin regenerierten. Entsprechend den gefundenen Wachstumsanomalien ließen sich cytologische teilweise Veränderungen der Kernsubstanz (Kernpyknose, Kernzerfall), des Protoplasmas (Verfettung, Hydratation) und der Hyphenmembran (Deformationen an den Zellengrenzen und Hyphenenden, Behinderung der Oidienbildung und- abschnürung) nachweisen.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1359-5997
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 33 (1977), S. 546-547 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary For 1 or 2 h following a gavage of milk or 300 mM CaCl2 (2 ml/100 g b.wt), rats had an increased liver calcium content when compared to rats receiving a deionized water gavage.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 33 (1977), S. 278-280 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Parathyroid hormone administration to thyroparathyroidectomized rats resulted in a significant reduction in inorganic phosphate content of a variety of striated muscles. Most other organs were unaffected. Much of the extra urinary phosphate present after parathyroid hormone stimulation may be released from the muscles.
    Type of Medium: Electronic Resource
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