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  • 1995-1999  (3)
  • 1975-1979
  • Affinity chromatography  (1)
  • Constipation  (1)
  • Hankel structure  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Diseases of the colon & rectum 38 (1995), S. 509-513 
    ISSN: 1530-0358
    Schlagwort(e): Colon ; Innervation ; Nerves ; Muscle ; Immunohistochemistry ; Constipation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract PURPOSE: This study was designed to investigate neural and muscular features of the colonic wall in patients with severe idiopathic constipation. METHODS: By using quantitative immunohistochemistry, resected specimens from 14 patients with idiopathic chronic constipation and 17 nonobstructed cancer controls were studied. RESULTS: Routine histology revealed no significant histologic abnormality throughout the colon apart from four cases of melanosis coli. Ratio of the thickness of circular to longitudinal muscle was significantly lower in the left colon in constipated subjects. The myenteric plexus appeared morphologically normal in all subjects. S-100 protein, which stains neuronal supporting tissues, demonstrated an increase in the proportion of neural tissue in the myenteric plexus. There was an increased number of PGP-9.5 immunoreactive nerve fibers in the muscularis propria in constipated patients, and this was significantly higher in the ascending and descending colon. CONCLUSION: Intractably constipated patients have alterations in the neural composition of the colonic myenteric plexus and innervation of the circular muscle.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    BIT 39 (1999), S. 757-779 
    ISSN: 1572-9125
    Schlagwort(e): Overdetermined linear systems ; rank reduction ; Hankel structure ; Toeplitz structure ; structured total least norm ; total least squares ; 1-norm ; 2-norm ; singular value decomposition
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Abstract The structure preserving rank reduction problem arises in many important applications. The singular value decomposition (SVD), while giving the closest low rank approximation to a given matrix in matrix L 2 norm and Frobenius norm, may not be appropriate for these applications since it does not preserve the given structure. We present a new method for structure preserving low rank approximation of a matrix, which is based on Structured Total Least Norm (STLN). The STLN is an efficient method for obtaining an approximate solution to an overdetermined linear system AX ≈ B, preserving the given linear structure in the perturbation [E F] such that (A + E)X = B + F. The approximate solution can be obtained to minimize the perturbation [E F] in the L p norm, where p = 1, 2, or ∞. An algorithm is described for Hankel structure preserving low rank approximation using STLN with L p norm. Computational results are presented, which show performances of the STLN based method for L 1 and L 2 norms for reduced rank approximation for Hankel matrices.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Protoplasma 184 (1995), S. 104-110 
    ISSN: 1615-6102
    Schlagwort(e): NADH oxidase ; Thermostable flavoprotein ; Affinity chromatography ; Codon usage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An NADH oxidase purified from the extreme thermophileThermus thermophilus HB8 is a monomeric flavoprotein with a 1 ∶ 1 ratio of flavin-adenine dinucleotide (FAD) to the polypeptide chain. It catalyzes in vitro the oxidation of reduced NADH or NADPH with the formation of H2O2. The gene encoding the NADH oxidase fromT. thermophilus HB8 was cloned, and its nucleotide sequence was determined. The molecular mass of 22,749 Da, as deduced from thenox gene, agrees with that of the purified NADH oxidase fromT. thermophilus HB8, as estimated by mass spectrometry. Thenox gene does not contain a GX4GK consensus sequence typical for nucleotide binding proteins. Thenox gene was overexpressed inEscherichia coli, and a protocol for the rapid purification of theE. coli-borneT. thermophilus NADH oxidase or its His6-tagged analogue was developed by using thermal denaturation step and affinity chromatography.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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