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  • 1
    Electronic Resource
    Electronic Resource
    Growth and diet dependant structural adaptations of the digestive tract in juvenile grass carp (Ctenopharyngodon idella, Val.) (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 11 (1977), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The morphology of the intestinal epithelium is described and compared with published data. In the gut of this stomachless fish, three zones can be distinguished based on light and electron microscopic observations of absorptive cells: a rostral zone with numerous fat droplets, a second zone with large P.A.S.-positive supranuclear vacuoles and pinocytotic vesicles, and a third zone without these characteristics. In these zones deep invaginations of the cell membrane is a feature of the basal part of the cells. The presence of pinocytotic vesicles in the second zone is considered evidence of the uptake of undigested protein-like substances.Juvenile grass carp from 15 to 335 days, fed on either animal or vegetable food, were measured. The growth rates indicate that animal food stimulates rapid growth. The data also show a vegetable diet causes a slight increase in relative gut length, mainly in the length of the first zone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1977.tb04110.x
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  • 2
    Electronic Resource
    Electronic Resource
    The carp homeobox gene Ovx1 shows early expression during gastrulation and subsequently in the vagal lobe, the facial lobe and the ventral telencephalon (1998)
    Springer
    Development genes and evolution 208 (1998), S. 56-59 
    Details
    ISSN: 1432-041X
    Keywords: Key words Carp ; Ovx ; Neural development ; Facial lobe ; Vagal lobe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The homeobox gene Carp-Ovx1 shows similarity to vertebrate and invertebrate Ovx genes and to Drosophila unplugged. Its expression pattern was studied by in situ hybridization in carp embryos and juveniles. During segmentation, expression becomes gradually limited to the neural tube. In juveniles up to 9 weeks old, cells in the ventral telencephalon, the facial lobe and the vagal lobe show Ovx1 expression, confining expression to parts with chemosensory projections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050154
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation of carp cDNA clones, representing developmentally-regulated genes, using a subtractive-hybridization strategy (1996)
    Springer
    Development genes and evolution 205 (1996), S. 460-467 
    Details
    ISSN: 1432-041X
    Keywords: Teleost development ; Subtractive hybridization ; Development-related RNAs ; Molecular markers ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A subtractive-hybridization technique, combined with differential screenings and subsequent whole mount in situ hybridization (ISH) reactions, was used to isolate novel cDNA clones representing developmentally-regulated genes of carp. Small-scale differential screenings of an oocyte and a segmentation-stage cDNA library using oocyte-specific and segmentation stage-specific enriched probes, yielded 75 positive clones. ISH screening showed that 65% (15) of the oocyte-stage clones and 50% (26) of the segmentation-stage clones were indeed stage-specific. Partial sequence analysis suggests that approximately 65% of the 41 stage-specific clones represent novel genes. In addition, an Otxl clone was isolated. Two novel clones and the Otxl clone are of special interest for developmental studies. The clones represent genes that are locally expressed during embryonic development. The expression patterns of Otxl and one of the novel clones suggest functions in specification of the anterior-posterior axis. The three clones provide molecular markers for the study of gastrulation and the patterning of the a-p axis in teleosts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00377227
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Regional functional differentiation in the gut of the grasscarp, Ctenopharyngodon idella (Val.) (1979)
    Springer
    Histochemistry and cell biology 64 (1979), S. 235-249 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A regional differentiation — reflecting structural differences — of the intestine of larval and juvenile grasscarps can be illustrated by studying the activity of alkaline phosphatase and the uptake of orally administered horseradish peroxidase. Pinocytosis of peroxidase takes place in a welldefined area of about 23% of the length of the gut (segment II). Neither the rostral ±68% (segment I) nor the caudal ±9% (segment III) shows absorption of the enzyme. Alkaline phosphatase activity, mainly localized at the microvilli of the enterocytes is high in the first segment of the gut and low in the second segment. In larvae, the activity decreases sharply at the transition from segment I to segment II. The activity is weak or absent in the caudal third segment. Quantitative histochemical data are confirmed by biochemical analyses. Alkaline phosphatase activity is found all over the mucosal folds of the first segment, with relatively weak activity at the base and at the tip of the folds. This may be related to a renewal of the epithelium. Our results suggest that active absorption of digested food takes place mainly in the rostral first segment, while the uptake of macromolecules by pinocytosis is a function of the second segment. Comparison of the results with information available in literature leads to a rejection of the hypothesis that the uptake of protein macromolecules in Cyprinids is to be attributed to the absence of a stomach and therefore to an inefficient digestion of proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495024
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  • 5
    Electronic Resource
    Electronic Resource
    The ultrastructure and renewal of the intestinal epithelium of the juvenile grasscarp, Ctenopharyngodon idella (Val.) (1978)
    Springer
    Cell & tissue research 187 (1978), S. 181-200 
    Details
    ISSN: 1432-0878
    Keywords: Intestine ; Teleost ; Epithelium ; Renewal ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The intestinal absorptive epithelium of starved and fed fish has been studied electron microscopically. After feeding, cells of the proximal segment of the intestine show morphological characteristics of lipid absorption. Absorptive cells in the middle segment contain many pinocytotic vesicles in both fasted and fed specimens. Absorption of protein macromolecules is supposed to be one of the main functions of this part of the gut. In the most caudal part of the intestine, absorptive cells carry relatively few and short microvilli. The proximal and distal segments show structural indications of a function in osmoregulation. The renewal of the epithelium has been studied with light microscopic autoradiography, using tritiated thymidine. The intestinal mucosal fold epithelium represents a cell renewal system. The cells proliferate at the base of the fold and migrate towards the apex in 10–15 days at 20° C. The functional absorptive cells proved to be generally present in the intestinal epithelium, including the proliferative area. Undifferentiated cells have not been identified. The results will be compared with data on absorption of lipid and protein macromolecules in teleostean and mammalian intestines and with descriptions of the cell renewal system in the mammalian intestine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224364
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    Paper (German National Licenses)
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