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  • 1
    Electronic Resource
    Electronic Resource
    The carp homeobox gene Ovx1 shows early expression during gastrulation and subsequently in the vagal lobe, the facial lobe and the ventral telencephalon (1998)
    Springer
    Development genes and evolution 208 (1998), S. 56-59 
    Details
    ISSN: 1432-041X
    Keywords: Key words Carp ; Ovx ; Neural development ; Facial lobe ; Vagal lobe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The homeobox gene Carp-Ovx1 shows similarity to vertebrate and invertebrate Ovx genes and to Drosophila unplugged. Its expression pattern was studied by in situ hybridization in carp embryos and juveniles. During segmentation, expression becomes gradually limited to the neural tube. In juveniles up to 9 weeks old, cells in the ventral telencephalon, the facial lobe and the vagal lobe show Ovx1 expression, confining expression to parts with chemosensory projections.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050154
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of carp cDNA clones, representing developmentally-regulated genes, using a subtractive-hybridization strategy (1996)
    Springer
    Development genes and evolution 205 (1996), S. 460-467 
    Details
    ISSN: 1432-041X
    Keywords: Teleost development ; Subtractive hybridization ; Development-related RNAs ; Molecular markers ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A subtractive-hybridization technique, combined with differential screenings and subsequent whole mount in situ hybridization (ISH) reactions, was used to isolate novel cDNA clones representing developmentally-regulated genes of carp. Small-scale differential screenings of an oocyte and a segmentation-stage cDNA library using oocyte-specific and segmentation stage-specific enriched probes, yielded 75 positive clones. ISH screening showed that 65% (15) of the oocyte-stage clones and 50% (26) of the segmentation-stage clones were indeed stage-specific. Partial sequence analysis suggests that approximately 65% of the 41 stage-specific clones represent novel genes. In addition, an Otxl clone was isolated. Two novel clones and the Otxl clone are of special interest for developmental studies. The clones represent genes that are locally expressed during embryonic development. The expression patterns of Otxl and one of the novel clones suggest functions in specification of the anterior-posterior axis. The three clones provide molecular markers for the study of gastrulation and the patterning of the a-p axis in teleosts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00377227
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  • 3
    Electronic Resource
    Electronic Resource
    Growth and diet dependant structural adaptations of the digestive tract in juvenile grass carp (Ctenopharyngodon idella, Val.) (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish biology 11 (1977), S. 0 
    Details
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The morphology of the intestinal epithelium is described and compared with published data. In the gut of this stomachless fish, three zones can be distinguished based on light and electron microscopic observations of absorptive cells: a rostral zone with numerous fat droplets, a second zone with large P.A.S.-positive supranuclear vacuoles and pinocytotic vesicles, and a third zone without these characteristics. In these zones deep invaginations of the cell membrane is a feature of the basal part of the cells. The presence of pinocytotic vesicles in the second zone is considered evidence of the uptake of undigested protein-like substances.Juvenile grass carp from 15 to 335 days, fed on either animal or vegetable food, were measured. The growth rates indicate that animal food stimulates rapid growth. The data also show a vegetable diet causes a slight increase in relative gut length, mainly in the length of the first zone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1095-8649.1977.tb04110.x
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  • 4
    Electronic Resource
    Electronic Resource
    Regional functional differentiation in the gut of the grasscarp, Ctenopharyngodon idella (Val.) (1979)
    Springer
    Histochemistry and cell biology 64 (1979), S. 235-249 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A regional differentiation — reflecting structural differences — of the intestine of larval and juvenile grasscarps can be illustrated by studying the activity of alkaline phosphatase and the uptake of orally administered horseradish peroxidase. Pinocytosis of peroxidase takes place in a welldefined area of about 23% of the length of the gut (segment II). Neither the rostral ±68% (segment I) nor the caudal ±9% (segment III) shows absorption of the enzyme. Alkaline phosphatase activity, mainly localized at the microvilli of the enterocytes is high in the first segment of the gut and low in the second segment. In larvae, the activity decreases sharply at the transition from segment I to segment II. The activity is weak or absent in the caudal third segment. Quantitative histochemical data are confirmed by biochemical analyses. Alkaline phosphatase activity is found all over the mucosal folds of the first segment, with relatively weak activity at the base and at the tip of the folds. This may be related to a renewal of the epithelium. Our results suggest that active absorption of digested food takes place mainly in the rostral first segment, while the uptake of macromolecules by pinocytosis is a function of the second segment. Comparison of the results with information available in literature leads to a rejection of the hypothesis that the uptake of protein macromolecules in Cyprinids is to be attributed to the absence of a stomach and therefore to an inefficient digestion of proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495024
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  • 5
    Electronic Resource
    Electronic Resource
    The identification of the thyrotropic cells in the adenohypophysis of the cichlid fish Cichlasoma biocellatum and the role of these cells and of the thyroid in osmoregulation (1971)
    Springer
    Cell & tissue research 121 (1971), S. 82-92 
    Details
    ISSN: 1432-0878
    Keywords: Thyrotrops ; Teleost ; Thyroid ; Prolactin ; Osmoregulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to identify the thyrotropic cells in the pituitary gland of Cichlasoma biocellatum, adult animals were treated with thyroxine. Periodic acid Schiff (PAS)- and aldehyde fuchsin (AF)-positive, and Alcian blue (AB)-negative basophils in the meso-adenohypophysis as well as the thyroid were strongly inactivated by such treatment. Since other cell types in the adenohypophysis were not affected, it is concluded that the PAS-positive and AB-negative basophils in the meso-adenohypophysis are the thyrotrops. In normal animals the thyrotrops and the thyroid are very active. Propylthiouracil did not cause a further activation of TSH cells. Keeping the animals in 25% sea-water caused a strong inactivation of the prolactin cells, the thyrotrops and the thyroid. In deionized water the prolactin cells were stimulated, but the thyroid was less active than in controls. Injections of ovine prolactin did not enhance the activity of the thyroid in salt water animals. It is suggested that in Cichlasoma biocellatum prolactin does not have a direct stimulatory effect on the thyroid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330919
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  • 6
    Electronic Resource
    Electronic Resource
    The effects of osmotic experiments and prolactin on the mucous cells in the skin and the ionocytes in the gills of the teleost Cichlasoma biocellatum (1971)
    Springer
    Cell & tissue research 121 (1971), S. 93-101 
    Details
    ISSN: 1432-0878
    Keywords: Prolactin ; Mucous cells ; Ionocytes ; Cichlasoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Adult Cichlasoma biocellatum were kept in deionized water, 25% sea-water and tap-water. The mucous cells in the epithelium of the gills and the buccal floor were not affected by the osmotic experiments. In animals kept in deionized water the mucous cells in the skin remained unaffected, but the ionocytes (chloride cells) in the gills were strongly stimulated. Keeping the animals in salt water led to a strong regression of the epidermis including the mucous cells, and of the ionocytes. The regression could be counteracted by prolactin injections. The changes in the skin and the ionocytes could be correlated with the activity of the prolactin producing cells in the adenohypophysis. It is suggested that the epidermis and its mucous cells as well as the ionocytes are under prolactin control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330920
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  • 7
    Electronic Resource
    Electronic Resource
    The cell types in the adenohypophysis of the cichlid fish Cichlasoma biocellatum regan, with special attention to its osmoregulatory role (1971)
    Springer
    Cell & tissue research 118 (1971), S. 113-126 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Teleost ; Prolactin cells ; Osmoregulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the adenohypophysis of Cichlasoma biocellatum, which may be divided into a pro-, meso-, and meta-adenohypophysis, seven cell types can be distinguished. Treatment with deionized- and salt water effects three of these cell types. The rostral acidophils, which were proved to produce prolactin by means of an immunochemical method, are distinctly stimulated in deionized water and markedly inactivated in 25% sea-water. The putative ACTH cells, bordering neurohypophysial branches in the pro-adenohypophysis, are moderately stimulated by both treatments. The presumptive TSH cells, which in control animals appear to be very active, do not show marked changes in deionized water; they are strikingly inactivated, however, in salt water specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331770
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  • 8
    Electronic Resource
    Electronic Resource
    Proliferation and differentiation of intestinal epithelial cells during development of Barbus conchonius (Teleostei, Cyprinidae) (1984)
    Springer
    Cell & tissue research 236 (1984), S. 207-216 
    Details
    ISSN: 1432-0878
    Keywords: Development ; Enterocytes ; Fish ; Mitosis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The processes of proliferation, cell division and differentiation of intestinal epithelial cells have been studied during development of the fish, Barbus conchonius. On the 3rd day, nearly all cells of the presumptive gut proliferate. Once the intestinal epithelium begins to differentiate, a decreasing percentage of proliferative cells can be found. On the 7th day, when intestinal folds start to develop, the proliferative cells become restricted to the future basal parts of the folds. Ultrastructural examination of 3H-thymidine-labeled cells and mitotic cells of 6-day-old larvae shows that functional enterocytes are proliferative. The same feature is suggested for older fish. Proliferating undifferentiated “dark” cells, characterized by many free ribosomes and a few organelles, are also present in the intestinal epithelium of larval fish; they are considered to be stem cells, mainly for goblet cells. Proliferating goblet cells and enteroendocrine cells were not observed. The latter cell type is scarce and has a long turnover time. A common feature of all these dividing cells is the presence of isolated spherical to cylindrical lamellar structures which may have lost contact with the cell membrane during prophase; they probably regain this contact by fusion with the cell membrane at the end of mitosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216533
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  • 9
    Electronic Resource
    Electronic Resource
    The ultrastructure of the uterine epithelium of the pig during the estrous cycle and early pregnancy (1986)
    Springer
    Cell & tissue research 246 (1986), S. 81-89 
    Details
    ISSN: 1432-0878
    Keywords: Uterus ; Epithelium ; Ultrastructure ; Pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the endometrial epithelium of the pig was studied during the estrous cycle and early pregnancy up to implantation. Special attention was given to the luminal epithelium and morphological indications of protein synthesis. Although the general morphology of the luminal and glandular epithelia is similar (both tissues consist of secretory cells and ciliated cells at all the stages studied), it appears that the two epithelia should be considered as two functionally different units in the pre-implantation period. Morphological evidence suggests the presence of at least three different secretory products within luminal epithelial cells; they are released at different times, i.e. at estrus, between day 8 and 10 and after day 11. The glandular epithelium shows release of secretory products from day 10–11. Increasing amounts of glycogen were found within epithelial cells, especially in pregnant gilts from day 12. The possible significance of secretory activity of the epithelium is discussed in relation to the development of the embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219003
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  • 10
    Electronic Resource
    Electronic Resource
    The ultrastructure and renewal of the intestinal epithelium of the juvenile grasscarp, Ctenopharyngodon idella (Val.) (1978)
    Springer
    Cell & tissue research 187 (1978), S. 181-200 
    Details
    ISSN: 1432-0878
    Keywords: Intestine ; Teleost ; Epithelium ; Renewal ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The intestinal absorptive epithelium of starved and fed fish has been studied electron microscopically. After feeding, cells of the proximal segment of the intestine show morphological characteristics of lipid absorption. Absorptive cells in the middle segment contain many pinocytotic vesicles in both fasted and fed specimens. Absorption of protein macromolecules is supposed to be one of the main functions of this part of the gut. In the most caudal part of the intestine, absorptive cells carry relatively few and short microvilli. The proximal and distal segments show structural indications of a function in osmoregulation. The renewal of the epithelium has been studied with light microscopic autoradiography, using tritiated thymidine. The intestinal mucosal fold epithelium represents a cell renewal system. The cells proliferate at the base of the fold and migrate towards the apex in 10–15 days at 20° C. The functional absorptive cells proved to be generally present in the intestinal epithelium, including the proliferative area. Undifferentiated cells have not been identified. The results will be compared with data on absorption of lipid and protein macromolecules in teleostean and mammalian intestines and with descriptions of the cell renewal system in the mammalian intestine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224364
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