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  • 1995-1999  (2)
  • 1970-1974  (2)
  • Key words Cell cycle  (2)
  • Unit recording  (2)
  • 1
    ISSN: 1432-0533
    Keywords: Key words Cell cycle ; Ki-67 ; Apoptosis ; Hippocampus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Recent studies, showing that cell cycle-related nuclear proteins p105 and Ki-67 are associated with Alzheimer’s disease (AD)-related cytoskeletal pathology, suggested that these proteins, in addition to their functions in regulating the cell cycle, may have more specialised functions in the adult nervous system. In order to test this hypothesis we studied the expression of the cell cycle-related proteins Ki-67, pCNA and p53 in the hippocampi of 33 subjects, including some with AD or other neurodegenerative disorders and some with no neurological disease. By immunohistochemistry we found nuclear expression of Ki-67 in all subregions of the hippocampus, with the highest levels in the dentate gyrus. Both neurons and glial cells expressed this protein. The proportion of cells positive for Ki-67 and the distribution pattern varied considerably depending on the pathological diagnosis. Neuronal nuclear expression of Ki-67 was increased in AD but was also elevated in young Down’s syndrome subjects and in those with Pick’s disease. Expression of this protein was therefore not AD-specific. We did not find nuclear pCNA or p53 expressed in our patient groups. Contrary to previous studies AD-type neurofibrillary tangles were not labelled with any of the cell cycle markers used. The presence of nuclear Ki-67 expression indicates that some hippocampal neurons are not in the quiescent G0 phase but have re-entered the cell cycle. The absence of nuclear pCNA or p53 suggests that the cycle is arrested in G1. The significance of our findings and their relationship to the production of neurodegenerative cell death via an apoptotic mechanism are discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Acta neuropathologica 94 (1997), S. 6-15 
    ISSN: 1432-0533
    Keywords: Key words Cell cycle ; Alzheimer’s disease ; Down’s syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Using immunohistochemistry we have analysed the nuclear expression of cyclins A, B, D, and E in neurones in the hippocampi of control subjects and patients suffering from various neurodegenerative disorders including Alzheimer’s disease (AD). Cyclins A and D could not be detected but varying degrees of cyclin E expression were found in all patient groups including control subjects. Cyclin B expression was not detected in control subjects but it was expressed in the subiculum, dentate gyrus and CA1 region in patients with AD-type pathology and in the CA2 region and the dentate gyrus of cases of Pick’s disease. These reults suggest that some neurones may have re-entered the cell cycle. The expression of cyclin E without cyclin A expression may indicate an arrest in G1 with the possibility of re-differentiation and exit from G1 to G0. The expression pattern of cyclin E indicates that re-entry into the cell cycle is possible even in control patients, but it is accentuated in patients with AD-related pathology. However, cyclin B was only expressed in AD patients and occurred in areas that were severely affected by pathology. Neurones with cyclin B-reactive nuclei in AD were AT8 positive but did not contain fully developed tangles. In neurones, where cyclin B is expressed, it would appear that the G1/S checkpoint has been bypassed and that the cell cycle is arrested in G2. It is proposed that these neurones do not have the opportunity for subsequent re-differentiation. Since factors known to be present in G2 seem to be responsible for microtubule destabilisation and hyperphosphorylation of tau we hypothesise that cell cycle disturbances may be important in the pathogenesis of AD.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 17 (1973), S. 315-332 
    ISSN: 1432-1106
    Keywords: Red nucleus ; Unit recording ; Motor cortex ; Topographical organization ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A topographical study of the cortico-rubrospinal pathway was conducted in cats anesthetized with chloralose. Extracellular unit recordings were made from cells in the red nucleus projecting to the spinal cord. They were identified by antidromic invasion following stimulation of their axones at the 2nd cervical and 9th thoracic levels of the spinal cord. I. The pericruciate cortical regions from which spikes could be induced in rubrospinal neurons were limited to the lateral part of the anterior sigmoid gyrus, the lateral sigmoid gyrus and the anterior part of the posterior sigmoid gyrus. No responses were obtained from stimulation of the medial part of the anterior sigmoid gyrus or the gyrus proreus. Compared to the somatotopic organization of the motor cortex for the cat described by Woolsey (1958), our results show that the rubrospinal cells receive projections from the motor cortex controlling proximal and distal muscles but not axial muscles. II. Neurons projecting to the cervico-thoracic cord receive afferents from the lateral part of the anterior sigmoid gyrus and the lateral sigmoid gyrus whereas those projecting to the lumbo-sacral cord receive projections from the entire surface of the sigmoid gyrus except the medial part of the anterior sigmoid gyrus and the gyrus proreus. III. A latero-medial organization of cells within the red nucleus was found according to the origin of their cortical afferents. Rubrospinal neurons with fibers terminating in the cervical or thoracic cord receive projections from the motor cortex controlling the proximal musculature of the forelimb when they are located in the dorso-lateral region of the nucleus and the entire forelimb motor cortex when they are located in the medial part of the nucleus. It is suggested that this organization may indicate a control of proximal forelimb musculature by dorsolateral rubrospinal cells and distal musculature by medial cells. IV. Rubrospinal cells placed medially in the nucleus receive more convergent projections (i.e. from a greater cortical surface) than cells placed more laterally. It was shown that for certain cells the convergence occurs in the direct pathway. These results are discussed in terms of a functional organization allowing coordinated movements of different segments of a single limb or of different limbs.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 14 (1972), S. 363-371 
    ISSN: 1432-1106
    Keywords: Rubrospinal cells ; Unit recording ; Topographical organization ; Conduction velocity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mapping of cells at the origin of the rubrospinal tract was conducted in the cat. 1. Rubrospinal neurons sending efferents to cervico-thoracic segments of the spinal cord are located in the dorso-medial part of the nucleus. These neurons are especially medial in the caudal planes and especially dorsal in the rostral planes. Neurons with efferents terminating at the level of lumbo-sacral segments of the cord occupy the ventro-lateral part of the nucleus. These neurons are especially lateral in the caudal planes and especially ventral in the rostral planes. The limit between these two cell populations is clear in the caudal and middle thirds of the nucleus but considerable overlap is seen in the rostral third. These results agree with the anatomical findings of Pompeiano and Brodal (1957). 2. For the population of lumbar neurons the conduction velocities ranged from 31 m/sec to more than 120 m/sec with a mean of 85 m/sec. 3. Rubrospinal cells are found throughout the nucleus. The most caudal planes are essentially composed of cells with rapidly conducting fibers whereas in the middle and rostral planes a cell population with increasingly slower conducting fibers appears. The results of the present study are discussed in relation to classical data on the magnocellular and parvocellular divisions of the red nucleus. 2.The third author acknowledge the personal support of the Medical Research Council of Canada.
    Type of Medium: Electronic Resource
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