ISSN:
1420-908X
Keywords:
Key words: Mast cells — TNFα— Neutrophils — Leukotrienes — Prostaglandins
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract. Objective and Design: In the present study, we investigated the role of mast cells in a model of polyacrylamide gel (PAG)-induced inflammation in mice.¶Subjects: Balb/c mice and two strains of mast cell deficient mice (WBB6F1/J-W/Wv, WCB6F1/J-S1/S1d).¶Treatment: Various quantities of polyacrylamide gel (Bio-Gel P4) were injected subcutaneously in the backs of mice.¶Methods: Five hours after the injection of PAG the animals were euthanized, the injection sites lavaged and levels of LTB4, PGE2, TNFα and cells were determined.¶Results: Subcutaneous injection of PAG caused a time-dependent response characterized by the accumulation of inflammatory cells peaking at 10 h and the formation of LTB4, PGE2 and TNFα, peaking at 5 h. PAG injection into W/Wv or SL/SLd mice (mice lacking mast cells) resulted in an attenuated response, i.e. LTB4 levels were reduced by 60% and minimal cell influx was seen. The lack of mast cells caused about a 30% reduction in the levels of TNFα found.¶Conclusions: These data suggest that mast cells play a prominent role in the PMN influx, TNFα production and eicosanoid formation in the PAG-induced inflammatory response.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s000110050292
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