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  • 1
    Electronic Resource
    Electronic Resource
    Bingley : Emerald
    International journal of health care quality assurance 10 (1997), S. 254-259 
    ISSN: 0952-6862
    Source: Emerald Fulltext Archive Database 1994-2005
    Topics: Medicine , Economics
    Notes: The use of patient questionnaires has increased widely in recent years. Their purpose, to incorporate patient perspectives into the orientation and design of health care, is, of course, commendable. However, the survey methods themselves have been less adequate, both in terms of validity and reliability, and with respect to the potential for using the results to improve the quality of health care. Presents a pilot study at three departments of ophthalmology in Sweden, involving a new method which meets reasonable demands for validity and reliability, and is explicitly change-oriented.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature America Inc.
    Nature biotechnology 17 (1999), S. 379-384 
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] The Coprinus cinereus (CiP) heme peroxidase was subjected to multiple rounds of directed evolution in an effort to produce a mutant suitable for use as a dye-transfer inhibitor in laundry detergent. The wild-type peroxidase is rapidly inactivated under laundry conditions due to the high pH ...
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Well diffracting crystals of rhamnogalacturonan acetylesterase from Aspergillus aculeatus have been obtained in two polymorphic modifications despite its heterogeneous glycosylation. The best-diffracting crystals (resolution 1.55 Å) are orthorhombic. The limit of the diffraction pattern of the other (trigonal) form is 2.5 Å. The ability of the enzyme to crystallize appears to depend on the glycosylation of the protein sample. This aspect has been investigated by mass spectrometry, which also showed that the orthorhombic crystals have the same glycosylation as the protein sample used in the crystallization.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 18 (1968), S. 40-41 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1072-8368
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] Laccase catalyses the oxidation of a variety of organic substrates coupled to the reduction of oxygen to water. It is widely believed to be the simplest representative of the ubiquitous blue multi-copper oxidase family. Laccase is implicated in a wide spectrum of biological activities and, in ...
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of biomedical science 3 (1996), S. 365-378 
    ISSN: 1423-0127
    Keywords: Retroviral vectors ; Gene therapy ; Stem cells ; Transcription ; Silence ; Inactivation ; Integration site ; Position effects ; Methylation ; Primer-binding site
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Although retroviral vector systems have been found to efficiently transduce a variety of cell types in vitro, the use of vectors based on murine leukemia virus in preclinical models of somatic gene therapy has led to the identification of transcriptional silencing in vivo as an important problem. Extinction of long-term vector expression has been observed after implantation of transduced hematopoietic cells as well as fibroblasts, myoblasts and hepatocytes. Here we review the influence of vector structure, integration site and cell type on transcriptional silencing. While down-regulation of proviral transcription is known from a number of cellular and animal models, major insight has been gained from studies in the germ line and embryonal cells of the mouse. Key elements for the transfer and expression of retroviral vectors, such as the viral transcriptional enhancer and the binding site for the tRNA primer for reverse transcription may have a major influence on transcriptional silencing. Alterations of these elements of the vector backbone as well as the use of internal promoter elements from housekeeping genes may contribute to reduce transcriptional silencing. The use of cell culture and animal models in the testing and improvement of vector design is discussed.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 261 (1968), S. 225-238 
    ISSN: 1432-1912
    Keywords: Iron Deficiency ; Iron Absorption ; Iron Complexes ; Rat ; Eisenmangel ; Eisen-Resorption ; Eisenkomplexe ; Ratte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung 1. Die Retention von freiem und komplexgebundenem 59Fe-Eisen wurde 6 Tage nach Verabreichung mit der Schlundsonde bei normalen und anämischen Ratten in einer Ganztiermeßanlage bestimmt. 2. Anämische Ratten nahmen fünfmal mehr Eisen auf als normale Tiere, wenn ionisiertes Eisen angeboten wurde. Alle geprüften Komplexbildner vermindern die gesteigerte Eisen-Retention anämischer Ratten. Bezogen auf die Werte der anämischen Gruppe (=100%) wurde die Retention durch EDTA um 87%, durch Citronensäure um 40%, durch Ascorbinsäure um 32% und durch Nicotinhydroxamsäure um 31% herabgesetzt. 3. In Gegenwart von Komplexbildnern ist die Eisen-Retention anämischer Tiere bei Citronensäure 8,7, von EDTA 6,3, von Nicotinhydroxamsäure 3,7 und von Ascorbinsäure 2,7 mal höher als bei normalen.
    Notes: Summary 1. In normal and anemic rats, the retention of free and complex-bound 59Fe-iron was measured with a whole body counter 6 days after its oral administration. 2. If the iron is administered in the ionic form to iron-deficient rats they show a retention of iron 5 times greater than that of normal animals. The increased iron retention of anemic rats is diminished by all complexing agents tested. EDTA reduced it by 87%, citric acid by 40%, ascorbic acid by 32% and nicotine hydroxamic acid by 31%. 3. When the iron is combined with citric acid its retention by iron-deficient rats was 8.7 times increased in comparison to normal rats, with EDTA 6.3 times, with nicotine hydroxamic acid 3.7 and with ascorbic acid 2.7 times.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0878
    Keywords: Key words: Leu-callatostatins ; Callatostatins ; Allatostatins ; Neuropeptides ; Peptide purification ; Immunocytochemistry ; Myoinhibition ; Codling moth ; Cydia pomonella (Tortricidae: Lepidoptera) (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. A neuroendocrine peptide of the Leu-callatostatin family, LPVYNFGL-NH2, has been isolated from tissue extracts of 5th instar larvae of the codling moth, Cydia pomonella (Lepidoptera). It is identical to a peptide previously isolated from the blowfly, Calliphora vomitoria (Diptera). The distribution of this peptide within the tissues of C. pomonella has been mapped by immunocytochemistry using antisera raised against LPVYNFGL-NH2. Midgut endocrine cells contain Leu-callatostatin immunoreactivity, as do several paired Leu-callatostatin neurones in the brain and ventral nerve cord. Within the visceral nervous system, the frontal ganglion contains four Leu-callatostatin neurones. Axons from these cells combine with others originating from neurones in the brain and project within the nervi cardiostomatogastrici to innervate the tissues of the foregut. In particular, the oesophageal valve has a prominent ring of Leu-callatostatin-immunoreactive fibres. The synthetic peptide, LPVYNFGL-NH2, has a potent reversible inhibitory effect in vitro on all visible forms of spontaneous contractile activity of the foregut, including closure of the oesophageal valve. Complete myoinhibition is observed at peptide concentrations from 10−10 to 10−16 M. These results, in conjunction with the results of similar studies on cockroaches, crickets and flies, suggest that the Leu-callatostatins are a ubiquitous family of insect neuroendocrine peptides with an important role in the control of gut motility.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Clinical and experimental medicine 135 (1962), S. 534-540 
    ISSN: 1591-9528
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung In zwei Versuchsgruppen von T.-vergifteten Ratten werden die präventive und kurative Wirkung von Re. geprüft. In einer dritten Versuchsgruppe wird an Hand von T.-vergifteten Ratten, die nicht mit Re. nachbehandelt wurden, der Frage spontan entstandener Granulome nachgegangen. Nach den tierexperimentellen morphologischen Ergebnissen dürfte dem Re. eine Leberschutzwirkung nicht abzusprechen sein. Das Re. verhindert zwar nicht den toxischen Leberschaden, aber es hemmt eindeutig die Gewebsschäden und fördert und unterstützt die Heilung solcher Schäden.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Weinheim [u.a.] : Wiley-Blackwell
    Materials and Corrosion/Werkstoffe und Korrosion 11 (1960), S. 397-398 
    ISSN: 0947-5117
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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