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  • 1995-1999  (378)
  • 1965-1969  (73)
  • 1900-1904
  • Analytical Chemistry and Spectroscopy  (451)
  • 1
    Electronic Resource
    Electronic Resource
    Sequencing electroblotted proteins by tandem mass spectrometry (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 1051-1055 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Electroblotting proteins separated by gel electrophoresis provides a suitable support for further manipulations and analysis of small amounts of relatively pure samples. On-membrane digestion, peptide mapping by mass spectrometry, and database searching offer sensitive and fast tools to identify the analyte. By providing sequence information, tandem mass spectrometry can go a step further, confirming the database identification, solving problems connected with post-translational modifications and sequence variations, or supplying the stretches of internal sequence necessary to synthesize an oligonucleotide probe for gene isolation. The viability of this approach was successfully evaluated using different tandem mass spectrometric techniques: metastable decomposition in a matrix-assisted laser desorption/ionization (MALDI) time-of-flight instrument with a curved-field reflectron; low energy collision-induced dissociation in a MALDI quadrupole ion trap mass spectrometer; and high energy collision-induced dissociation in a high-performance four-sector mass spectrometer with massive cluster-impact ionization.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091116
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  • 2
    Electronic Resource
    Electronic Resource
    Suppressed Ion Chromatographic Determination of Chloride in Concrete (1999)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 22 (1999), S. 245-246 
    Details
    ISSN: 0935-6304
    Keywords: Chloride ; suppressed ion chromatography ; concrete ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---No abstract
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 3
    Electronic Resource
    Electronic Resource
    Extraction of fluazinan residues from fruits by CO2 in the supercritical state (1997)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 20 (1997), S. 569-571 
    Details
    ISSN: 0935-6304
    Keywords: Supercritical Fluid Extraction (SFE) ; Gas chromatograph ; Pesticides residues ; Fluazinan ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240201010
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Dendrimers as pseudo-stationary phases in elektrokinetic chromatography (1995)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 18 (1995), S. 121-123 
    Details
    ISSN: 0935-6304
    Keywords: Electrokinetic chromatography (EKC) ; Pseudo-stationary phase ; Micelles ; Dendrimers ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240180212
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  • 5
    Electronic Resource
    Electronic Resource
    The mass spectra of some C-19 modified cholesteryl derivatives (1968)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 1 (1968), S. 567-581 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The mass spectra of twenty one C-19 modified cholesteryl derivatives have been determined and compared with results for related systems. In the case when the hydrogen(s) of the C-19 group have been replaced by other groups the metastable evidence shows that in those molecules with a C-3 hydroxyl group the loss of water followed by the loss of the C-19 group gives rise to an intense ion at m/e 353, whereas the loss of the C-19 group with hydrogen transfer to the ion, followed by the loss of water gives rise to the large ion at m/e 354. In contrast in the case of the C-3 acetates the main fragmentation is the loss of acetic acid followed by the loss of the C-19 group to give the ion at m/e 353. This is rationalised on the basis of results observed in other systems. In the case of the C-3 tosylates evidence for pyrolysis before electron impact fragmentation is presented. The subsequent fragmentation of the large ions at m/e 354, 353 and other m/e values in the high mass region is shown to be in line with earlier work.
    Additional Material: 22 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210010411
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  • 6
    Electronic Resource
    Electronic Resource
    Non-equivalence Magnetique en Resonance Nucleaire - IV. Influence de Plusieurs Types de Dissymetries sur les Deplacements Chimiques et les Couplages Relatifs a des Systemes —O—CH2—CH3 et —S—CH2—CH3 (1969)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 1 (1969), S. 19-26 
    Details
    ISSN: 0030-4921
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Magnetic non-equivalence of methylenic protons in A—CH2—CH3 groups were studied for several types of compounds. Results are compared with respect (1) to the nature of heteroatom A:A = O and A = S and (2) to the structure of the local dissymmetry element: asymmetric carbon, ketal group and allenic system. Influence of substitution is discussed in relation to conformational problems. Furthermore a variation of the methylene geminal coupling constant with substituents of carbon α to OEt is observed.
    Notes: La non-équivalence magnétique des protons méthyléniques de groupements A—CH2—CH3 est étudiée dans plusieurs séries de composés. Les effets sont comparés d'une part dans les séries A = O et A = S, d'autre part dans les composés présentant un carbone asymétrique, un carbone de type cétal ou une dissymétrie allénique. L'influence de la substitution sur le carbone en α de AEt est discutée en relation avec les conformations. Une variation de la constante de couplage entre les protons méthyléniques géminés en function de la nature des substituants lointains est mise en évidence.
    Additional Material: 6 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1270010106
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  • 7
    Electronic Resource
    Electronic Resource
    Nuclear quadrupole resonance and molecular orbital studies of charge distribution in H-bonded complexes of 2-chloro-4-nitrobenzoic acid (1995)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 33 (1995), S. 476-480 
    Details
    ISSN: 0749-1581
    Keywords: NQR ; 35Cl NQR ; MO theory ; H-bonds ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The charge distribution in hydrogen bridges of varying strengths in solid 2-chloro-4-nitrobenzoic acid complexes was studied using the 35Cl nuclear quadrupole resonance technique (NQR). The dependence of 35Cl NQR frequencies on the proton affinities was analysed. Molecular orbital calculations using atom superposition and electron delocalization molecular orbital theory showed that the trends of changes in the charge density on the chlorine, nitrogen and oxygen atoms, and on the binding energy and the bond order of NH and OH groups are consistent with the NQR results. Also, the agreement between theoretical and experimental results showed that the 3,5-dimethylpyridine complex represents the critical point at 50% proton transfer.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260330612
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  • 8
    Electronic Resource
    Electronic Resource
    Investigation of substituted dihydronaphthalenes using 1H and 13C NMR spectroscopy (1995)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 33 (1995), S. 592-595 
    Details
    ISSN: 0749-1581
    Keywords: 1H NMR ; 13C NMR ; substituted dihydronaphthalenes ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 1H and 13C NMR studies were carried out on the substituted dihydronaphthalene compounds 1,2-dihydro-8-isopropyl-6,7-dimethoxy-2-methylnaphthalene, 3-bromo-1, 2-dihydro-8-isopropyl-6, 7-dimethoxy-2-methylnaphthalene, 3, 4-dihydro-5-isopropyl-6, 7-dimethoxy-3-methyl-1(2H)-naphthalenone, 1, 2-dihydro-6, 7-dimethoxy-2, 8-dimethylnaphthalene, 3-bromo-1, 2-dihydro-6, 7-dimethoxy-2, 8-dimethylnaphthalene and 3, 4-dihydro-6, 7-dimethoxy-3, 5-dimethyl-1(2H)-naphthalenone. Complete assignments of the proton and carbon spectra were made using one- and two-dimensional NMR techniques including APT, COSY, NOESY and 1H-13C HETCOR spectroscopy.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260330717
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  • 9
    Electronic Resource
    Electronic Resource
    Separation and characterization of components of peptide libraries using on-flow coupled HPLC - NMR spectroscopy (1995)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 33 (1995), S. 857-863 
    Details
    ISSN: 0749-1581
    Keywords: peptides ; HPLC-NMR ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The efficiency of directly coupled HPLC - NMR spectroscopy for the separation and characterization of synthetic peptide libraries was investigated using a model system based on a mixture of all 27 tripeptide combinations of alanine (A), methionine (M) and tyrosine (Y) as the C-terminal amides. The 1H NMR spectra were obtained in real time using on-flow HPLC - NMR spectroscopy at 600 MHz and 21 of the 27 peptides were identified on the basis of chemical shifts and coupling constants, including the use of diagnostic values derived from 1H NMR spectra and 1H—13C and 1H—15N HMQC studies on authentic A—A—A—OH and Y—Y—Y—OH. The effects on 1H NMR chemical shifts of pH and of varying proportions of acetonitrile and water as occurred during the gradient elution HPLC run were evaluated. The HPLC - NMR approach could provide a facile method for investigating the relative efficiency of resin-based peptide synthesis for different amino acid combinations and should rapidly provide data suitable for deriving structure-retention time relationships for peptides.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260331103
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  • 10
    Electronic Resource
    Electronic Resource
    N-glycosylation site mapping of recombinant tissue plasminogen activator by micellar electrokinetic capillary chromatography (1995)
    New York, NY : Wiley-Blackwell
    Biomedical Chromatography 9 (1995), S. 59-67 
    Details
    ISSN: 0269-3879
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: This report describes the N-glycosylation mapping of recombinant tissue plasminogen activator (rt-PA) using micellar electrokinetic capillary chromatography. The carbohydrate structures were tentatively assigned by comparison with the anion-exchange fractionated oligosaccharides and by a comparison with previously reported data. The separation was shown to rely mainly on the degree of sialylation of the oligosaccharides, allowing a quantitative determination of the proportion of neutral and mono- to tetrasialylated structures. Significant differences in the oligosaccharide distribution of the two variants of rt-PA, which differ by the presence (type I) or the absence (type II) of oligosaccharides at the Asn-184 site, were observed. The distribution of the oligosaccharides at each of the rt-PA glycosylation sites was then determined. Glycopeptides were prepared by tryptic digestion of rt-PA and isolated using two consecutive chromatographic procedures. The glycopeptides were finally treated with N-glycanase, and the resulting oligosaccharides were analysed by capillary electrophoresis. Oligosaccharide mapping revealed that the Asn-448 and Asn-184 sites carry the same population of complex-type oligosaccharides but that the relative amounts of each oligosaccharide vary markedly. High-pH anion-exchange chromatography performed on the desialylated oligosaccharides at each glycosylation site showed that the degree of microheterogeneity was related not only to the degree of sialylation but also to structural differences in the oligosaccharide sequences. From the results as a whole, we concluded that the Asn-448 site contains a greater proportion of heavily sialylated structures and has a higher degree of microheterogeneity. The Asn-117 site was demonstrated to contain a significant number of monosialylated structures (18.4%) in addition to the high-mannose-type oligosaccharides.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bmc.1130090202
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