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Immunolabeled microtubules and microfilaments are visible in multiple cell layers of rye root tip sections (1996)

Ericson, M. E. ; Carter, J. V.

Springer

Protoplasma 191 (1996), S. 215-219

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ISSN: 1615-6102

Keywords: Laser scanning confocal microscopy ; Multiple cell layers ; Plant microtubules ; Plant microfilaments ; Roots ; Tissue clearing

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary A protocol was developed to observe plant microtubules and actin microfilaments in large tissue samples without physical sectioning. Rye (Secale cereale L. cv. Rymin) root tip pieces from two-day-old seedlings were fixed and processed for immunolabeling. Incubation times of 24–48 h were required to insure adequate penetration of fixatives, antibodies, and washing buffers. Clearing of the tissue with methyl salicylate reduced background auto-fluorescence that would otherwise interfere with the resolution of cytoskeletal structures. Microtubules or microfilaments in 5–7 cell layers were visualized using the optical-sectioning capability of laser scanning confocal microscopy (LSCM) and projected as three-dimensional images. The three-dimensional character of the cytoskeletal elements is retained when viewing stained cells of intact tissue. The net-like character of a microfilament array radiating out from a single point into the cytoplasm is maintained when the cells are stained in intact root tip pieces and imaging is accomplished in situ.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01281819

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The juxtaglomerular apparatus in fetal and newborn mice (1967)

Kaylor, C. T. ; Carter, J. M.

New York, NY [u.a.] : Wiley-Blackwell

The @Anatomical Record 159 (1967), S. 171-177

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ISSN: 0003-276X

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Medicine

Notes: Kidneys of timed fetal and newborn mice of the C57BL stock were sectioned and stained with a modified Bowie technique. The juxtaglomerular cells, indentifiable by their granularity and position, were recognized rather late in fetal life - three or four days before birth. By the eighth day postpartum, the granular cells of the JGA had reached a peak of differentiation and closely resembled the adult JGA in their number, size, granularity, and position. The mesangial cell was often but not always granulated. The granular cell indices (G.C.I.) of fetal, newborn, and adult mice are compared. The high G.C.I. in fetal kidneys in our studies should account for the high renin titre found by others in fetal kidney extracts.

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