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  • 1995-1999  (7)
  • 1935-1939
  • RFLP  (3)
  • PVTx properties  (2)
  • equation of state  (2)
  • 1
    ISSN: 1432-2242
    Schlagwort(e): Key words Potato virus A ; Potato virus Y ; Resistance gene ; RFLP ; Diploid potato
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The gene Ry adg that confers resistance to potato Y potyvirus (PVY) in the cultivated potato [Solanum tuberosum subsp. andigena, line 2x(v-2)7] is located on chromosome XI in a segment that contains three other known resistance genes in other syntenic solanaceous species. One of them is the gene N that controls resistance to tobacco mosaic tobamovirus in tobacco and has previously been isolated and sequenced. Three sequence-related, resistance gene-like (RGL) DNA fragments (354–369 bp) highly homologous to the gene N were PCR-amplified from the potato line 2x(v-2)7. Two RGL fragments (79 and 81% homologous to the N gene) co-segregated with Ry adg among the 77 F1 progeny tested. These RGLs may originate from a resistance gene family on chromosome XI. The potato line 2x(v-2)7 also expressed resistance to potato A potyvirus (PVA), which was controlled by another locus on chromosome XI mapped ca. 6.8 cM distal to Ry adg .
    Materialart: Digitale Medien
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  • 2
    ISSN: 1432-2242
    Schlagwort(e): Key words Potato virus Y ; Resistance gene ; Solanum tuberosum subsp. andigena ; Potato ; RFLP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The chromosomal location of the major gene Ry adg controlling extreme resistance to potato virus Y (PVY) in Solanum tuberosum subsp. andigena was identified by RFLP analysis of a diploid potato population. A total of 64 tomato and potato RFLP markers were screened with the bulked segregant analysis (BSA) on segregants extremely resistant, hypersensitive or susceptible to PVY. Four markers TG508, GP125, CD17 and CT168 at the proximal end of chromosome XI showed close linkage with extremely resistant phenotypes. TG508 was identified as the closest marker linked with the Ry adg locus with the maximum map distance estimated as 2.0 cM. The 4 markers linked with the Ry adg locus were tested on independent tetraploid and diploid potato clones and were subsequently found useful for marker-assisted selection for plants containing Ry adg .
    Materialart: Digitale Medien
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  • 3
    ISSN: 1432-2242
    Schlagwort(e): Key words Potato virus X ; Resistance gene ; Genetic mapping ; RFLP ; Solanaceae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The line IvP35 of the diploid (2n=2x=24) cultivated potato species Solanum phureja (family Solanaceae) expresses hypersensitive resistance (H) to potato X potexvirus (PVX). In this study, a diploid potato population was produced using IvP35 as the male parent and a diploid line of S. tuberosum (87HW13.7) as the female parent and tested for resistance to PVX. Data indicated that H to PVX in IvP35 is a dominant, monogenically inherited trait controlled by a single gene, named Nx phu , that is in a simplex condition (Nxnx). RFLP analysis carried out on the progeny lines revealed 4 markers (CT220, TG328, CT112 and TG424) from the long arm of chromosome IX that were linked to the hypersensitive phenotype; the closest linkage was observed with the marker TG424. Previous authors have shown that the same region of chromosome IX contains the gene Sw-5 for resistance to tomato spotted wilt tospovirus in Lycopersicon peruvianum (Solanaceae).
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    International journal of thermophysics 17 (1996), S. 43-54 
    ISSN: 1572-9567
    Schlagwort(e): alternative refrigerant ; binary refrigerant mixtures ; HFC-32+HFC-125 system ; PVTx properties ; thermodynamic properties ; virial coefficients
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The experimental 156PVTx properties of an important binary refrigerant mixture, HFC-32 (difluoromethane)+HFC-125 (pentafluorethane), have been measured for three compositions, i.e., 50, 60, and 80 wt% HFC-32, by a constant-mass-method coupled with expansion procedure in an extensive range of temperaturesT from 320 to 440 K, of pressuresP from 1.8 to 5.3 M Pa, and of densities p from 50 to 124 kg · m−3. The experimental uncertainties of the present measurements are estimated to be within ±7 mK in temperature, ±2 kPa in pressure, ±0.2% in density and ±0.02 wt% of HFC-32. The sample purities are 99.998 wt% for HFC-32 and 99.99 wt% for HFC-125. Seventy-eight second and third virial coeflicients for temperatures from 320 to 440 K have been determined by the present measurements.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    International journal of thermophysics 20 (1999), S. 1639-1651 
    ISSN: 1572-9567
    Schlagwort(e): alternative refrigerant ; equation of state ; Helmholtz energy ; R-143a ; 1,1,1-trifluoroethane
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract A fundamental equation of state has been developed for 1,1,1-trifluoroethane (R-143a) using the dimensionless Helmholtz energy. The experimental thermodynamic property data, which cover temperatures from the triple point (161 K) to 433 K and pressures up to 35 MPa, are used to develop the present equation. These data are represented by the present equation within their reported experimental uncertainties: ±0.1% in density for both vapor and liquid phase P–ρ–T data, ±1% in isochoric specific heat capacities, and ±0.02% in the vapor phase speed-of-sound data. The extended range of validity of the present model covers temperatures from 160 to 650 K and pressures up to 50 MPa as verified by the thermodynamic behavior of the isobaric heat-capacity values over the entire fluid phase.
    Materialart: Digitale Medien
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  • 6
    ISSN: 1572-9567
    Schlagwort(e): alternative refrigerant ; equation of state ; hydrofluorocarbon ; R-32 ; R-125 ; second virial coefficient ; speed of sound
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The second virial coefficients, B, for difluoromethane (R-32, CH2F2) and pentafluoroethane (R-125, CF3CHF2) are derived from speed-of-sound data measured at temperatures from 273 to 343 K with an experimental uncertainty of ±0.0072%. Equations for the second virial coefficients were established, which are valid in the extensive temperature ranges from 200 to 400 K and from 240 to 440 K for R-32 and R-125, respectively. The equations were compared with theoretically derived second virial coefficient values by Yokozeki. A truncated virial equation of state was developed using the determined equation for the virial coefficients. The virial equation of state represents our speed-of-sound data and most of the vapor PρT data measured by deVries and Tillner-Roth within ±0.01 and ±0.1%, respectively.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    International journal of thermophysics 20 (1999), S. 97-106 
    ISSN: 1572-9567
    Schlagwort(e): alternative refrigerants ; binary refrigerant mixtures ; dew point pressures ; HFC-125+HFC-143a system ; PVTx properties ; saturated vapor densities ; virial coefficients
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract The experimental PVTx properties of a binary refrigerant mixture, R-125 (pentafluoroethane)+R-143a (1,1,1-trifluoroethane), have been measured for a composition of 50 mass% R-125 by a constant-mass method coupled with an expansion procedure in a range of temperatures from 305 to 400 K, pressures from 1.5 to 6.1 MPa, and densities from 92 to 300 kg·m−3. The experimental uncertainties of the present measurements are estimated to be within ±7.2 mK in temperature, ±3.0 kPa in pressure, ±0.12 kg·m−3 in density, and ±0.040 mass% in composition. The sample purities are 99.953 mass% for R-125 and 99.998% for R-143a. Seven saturated vapor densities and dew point pressures of the R-125+R-143a system were determined, on the basis of rather detailed PVTx properties measured in the vicinity of the saturation boundary as well as the thermodynamic behavior of isochores near saturation. The second and third virial coefficients for temperatures from 330 to 400 K were also determined.
    Materialart: Digitale Medien
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