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  • 1995-1999  (2)
  • 1905-1909
  • Column liquid chromatography  (1)
  • Key wordsEyes absent  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Determination of free aliphatic aldehydes in plasma by high-performance liquid chromatography of the 1,3-cyclohexanedione derivatives (1996)
    Springer
    Chromatographia 43 (1996), S. 501-506 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Aldehydes, aliphatic ; 1,3-Cyclohexanedione (CHD) ; Fluorimetric detection ; Plasma analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple and sensitive method is proposed for the measurement of aldehydes in plasma. The plasma samples are treated with 1,3-cyclohexanedione (CHD) and after addition of the internal standard (the CHD derivative of octaldehyde) are analysed by reversed-phase high-performance liquid chromatography with an acetonitrile-water gradient as the mobile phase. Detection is by fluorimetry with excitation at 395 nm and emission at 457 nm. In plasma samples spiked with aldehydes, the calibration curves showed good linearity in the range 0–10 μg mL−1. The recoveries of aldehydes were quantitative; coefficients of within-day and between-day variation were less than 13% and 11%, respectively. Applications to human and some animal plasma samples are reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02293001
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  • 2
    Electronic Resource
    Electronic Resource
    Homologs of animal eyes absent (eya) genes are found in higher plants (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 131-138 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsEyes absent ; Organ morphogenesis ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Homologs of the eyes absent (eya) gene in animals function at multiple stages in the development of organs. Their functional roles in the genetic network that regulates eye development in Drosophila have recently been extensively analyzed. A rice homolog of eya was identified from a cDNA library made from embryo RNA. The corresponding gene (OSEya1) encodes a conserved ED1 domain and a short N-terminal peptide. The ED1 domain of OSEya1 shows 25% identity and 36% similarity to the product of Drosophila eya. Mammalian and squid eya homologs show about 35% similarity to OSEya1. Homologous sequences were also found in the alfalfa EST database (53% identity and 65% similarity to OSEya1) and in the Arabidopsis genome sequence (63% identity). Therefore, eya homologs are present in both monocots and dicots. Three regions in the ED1 domain are well conserved in animals and plants. Plant eya products deduced from the nucleotide sequences also have short N-terminal peptides. The OSEya1 gene is located between the wx gene and the telomere on the short arm of chromosome 6. OSEya1 is expressed in the embryo, shoot apex, and caryopsis in rice. Expression in the embryo increases during embryogenesis until 7 days after pollination, with preferential localization in leaf primordia and the shoot apical meristem. Expression in the influorescence was observed in floral meristems. The functions of OSEya1 in higher plants are discussed and compared with those of their animal homologs. OSEya1 might regulate the morphogenesis of lateral organs as a subunit of a transcription factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051067
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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