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  • 1995-1999  (2)
  • 1905-1909
  • Column liquid chromatography  (1)
  • Particle bombardment  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Direct gene delivery into isolated microspores of rapeseed (Brassica napus L.) and the production of fertile transgenic plants (1998)
    Springer
    Plant cell reports 17 (1998), S. 323-328 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsBrassica napus ; Microspore culture ; Transgenic plant ; Particle bombardment ; Doubled haploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure for direct gene transfer into isolated microspores of rapeseed (Brassica napus L.) and the production of fertile transgenic plants is presented. By modifying the microspore culture method and adopting the firefly luciferase (Luc) gene as a non-destructive marker, we could obtain stably transformed androgenetic embryos from bombarded microspores. Luc-positive embryos were easily isolated from the large non-transformed population using a high-sensitivity bioluminescent image analyzer. PCR and Southern blot analyses confirmed that the introduced transgene was integrated stably into the genome of the selected embryos. Diploidized plants obtained from the haploid embryos were self-pollinated, and all of the offspring tested were Luc-positive, indicating rapid fixation of the transgene which is characteristic of doubled haploids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050401
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Determination of free aliphatic aldehydes in plasma by high-performance liquid chromatography of the 1,3-cyclohexanedione derivatives (1996)
    Springer
    Chromatographia 43 (1996), S. 501-506 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Aldehydes, aliphatic ; 1,3-Cyclohexanedione (CHD) ; Fluorimetric detection ; Plasma analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A simple and sensitive method is proposed for the measurement of aldehydes in plasma. The plasma samples are treated with 1,3-cyclohexanedione (CHD) and after addition of the internal standard (the CHD derivative of octaldehyde) are analysed by reversed-phase high-performance liquid chromatography with an acetonitrile-water gradient as the mobile phase. Detection is by fluorimetry with excitation at 395 nm and emission at 457 nm. In plasma samples spiked with aldehydes, the calibration curves showed good linearity in the range 0–10 μg mL−1. The recoveries of aldehydes were quantitative; coefficients of within-day and between-day variation were less than 13% and 11%, respectively. Applications to human and some animal plasma samples are reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02293001
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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