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  • 1995-1999  (4)
  • 1870-1879
  • 1830-1839
  • Demyelination  (2)
  • Denitrification  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Field method to determine N2O emission from nitrification and denitrification (1998)
    Springer
    Biology and fertility of soils 28 (1998), S. 51-55 
    Details
    ISSN: 1432-0789
    Keywords: Key words Nitrous oxide ; Nitrification ; Denitrification ; Soil cores ; Acetylene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Nitrous oxide (N2O) emissions via the nitrification (I nit) and denitrification (I den) pathways were successfully measured with in-field incubation of soil cores in preserving jars at 0 Pa and 5–10 Pa acetylene. From the incubations, fractions of nitrification – N2O over total N2O (I nit / I tot) – and denitrification – N2O over total N2O (I den / I tot) – were obtained. Actual field emissions of N2O via nitrification (F nit) and denitrification (F den) were calculated by multiplying the fractions from the incubation technique with the daily N2O emission (F day) determined with a direct soil cover method. The approach presented here was successful for a whole range of soil moisture conditions in intensive grassland. F nit and F den followed the trends of soil ammonium and soil nitrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050462
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Mechanistic model for nitrous oxide emission via nitrification and denitrification (1997)
    Springer
    Biology and fertility of soils 24 (1997), S. 231-238 
    Details
    ISSN: 1432-0789
    Keywords: Key words N2O ; Mechanistic model ; Nitrification ; Denitrification ; Michaelis-Menten kinetics ; Grassland ; Spatial variablity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seasonal and annual N2O fluxes from urine-affected pasture were approximated with a mechanistic model based on Michaelis-Menten kinetics. The model combined the effects of soil nitrate-N, soil ammonium-N, soil temperature and soil moisture (all from the top 5cm) to calculate N2O emissions from nitrification (F nit ) and denitrification (F den ), with total N2O emission being the sum of the two (F tot =F nit +F den ). Best results were obtained when different kinetic parameters were used for periods of constant soil moisture conditions and after heavy rainfalls when a rapid change of the soil moisture status occurred. Modelled N2O emissions over a year were within the range of uncertainties of measured N2O emissions. Results indicate that the spatial variability of N2O emissions at times when all the model inupt variables were constant may be related to microorganism growth dynamics or enzyme production rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050236
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Restricted infection with canine distemper virus leads to down-regulation of myelin gene transcription in cultured oligodendrocytes (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 312-318 
    Details
    ISSN: 1432-0533
    Keywords: Canine distemper virus ; Oligodendrocytes ; Myelin gene expression ; Demyelination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Canine distemper virus (CDV) induces oligodendroglial degeneration and multifocal demyelination in the central nervous system. The mechanism of oligodendrocyte degeneration is not understood but it has been shown that there is a restricted infection of these cells without viral protein production. Using a combination of immunocytochemistry and in situ hybridization we were able to demonstrate the transcription of the entire virus genome throughout the whole observation period (7–35 days after infection) in oligodendrocytes in CDV-infected brain cell cultures. Therefore, the lack of viral protein and particle production can not be explained on the basis of a defective viral transcription. The present study also shows that a restricted infection of oligodendrocytes with CDV down-regulates the transcription of the major myelin genes coding for proteolipid protein, myelin basic protein (MBP) and myelin-associated glycoprotein in a very similar way. Using densitometry for in situ hybridization products of MBP in populations of normal and infected oligodendrocytes, an effect could be observed long before morphological changes were detectable. The present results strongly suggest that demyelination in distemper is induced by a restricted CDV infection of oligodendrocytes which down-regulates the expression of a variety of cellular genes, in particular those coding for myelin proteins. Consequently, the infected cells are no longer able to synthesize all the membrane compounds which are necessary for maintaining their structural integrity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296516
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Restricted infection with canine distemper virus leads to down-regulation of myelin gene transcription in cultured oligodendrocytes (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 312-318 
    Details
    ISSN: 1432-0533
    Keywords: Key words Canine distemper virus ; Oligodendrocytes ; Myelin gene expression ; Demyelination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Canine distemper virus (CDV) induces oligodendroglial degeneration and multifocal demyelination in the central nervous system. The mechanism of oligodendrocyte degeneration is not understood but it has been shown that there is a restricted infection of these cells without viral protein production. Using a combination of immunocytochemistry and in situ hybridization we were able to demonstrate the transcription of the entire virus genome throughout the whole observation period (7–35 days after infection) in oligodendrocytes in CDV-infected brain cell cultures. Therefore, the lack of viral protein and particle production can not be explained on the basis of a defective viral transcription. The present study also shows that a restricted infection of oligodendrocytes with CDV down-regulates the transcription of the major myelin genes coding for proteolipid protein, myelin basic protein (MBP) and myelin-associated glycoprotein in a very similar way. Using densitometry for in situ hybridization products of MBP in populations of normal and infected oligodendrocytes, an effect could be observed long before morphological changes were detectable. The present results strongly suggest that demyelination in distemper is induced by a restricted CDV infection of oligodendrocytes which down-regulates the expression of a variety of cellular genes, in particular those coding for myelin proteins. Consequently, the infected cells are no longer able to synthesize all the membrane compounds which are necessary for maintaining their structural integrity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296516
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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