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  • 1995-1999  (2)
  • 42.60  (1)
  • Cryptic gene  (1)
  • 1
    ISSN: 1432-0649
    Keywords: 42.55 ; 42.60
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We report a comparative study of a pulsed as well as continuous-wave (cw) injection seeding of a Ti:Sapphire laser pumped by aQ-switched frequency-doubled Nd3+:YAG laser for achieving narrow spectral bandwidth. The results have indicated that the Ti:Sapphire laser using either a pulsed or a cw injection seeding could achieve efficient energy extraction in a narrow spectral bandwidth. In the case of pulsed injection seeding, the injection energy required for the complete injection seeding critically depended upon the timing of the Ti:Sapphire laser with respect to the delayed onset of the slave laser. On the other hand, in the case of cw injection seeding, the spectral bandwidth of the Ti:Sapphire laser was efficiently narrowed down to approximately 0.01 cm−1 with an injection power of less than 1 mW. In both types of injection seeding, characteristics observed experimentally were compared with those obtained by a numerical simulation code based on the one-dimensional rate-equation model.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Streptomyces setonii ISP5395 ; Cryptic gene ; Carotenogenesis ; Sigma factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In many species of actinomycetes, carotenogenesis can be photoinduced. The capacity to respond to photoinduction is, however unstable and, in various strains of Streptomyces, is lost at a relatively high frequency. In Streptomyces setonii ISP5395, which normally produces no carotenoids, carotenoid-producing mutants can be obtained following protoplast regeneration. We report here the characterization of a gene, crtS, which was isolated from one such mutant and can confer on wild-type S. setonii ISP5395 cells the capacity to synthesize carotenoids. Sequence analysis of crtS reveals an open reading frame, which shows homology to genes that encode alternative sigma factors in Bacillus subtilis. We propose that crtS encodes a sigma factor which is necessary for the expression of a cryptic gene(s) for carotenoid biosynthesis in S. setonii ISP5395.
    Type of Medium: Electronic Resource
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