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  • 1995-1999  (3)
  • Antifungal activity  (2)
  • Acinar cell carcinoma  (1)
  • Fetal pancreas  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A fungal chitinase gene fromRhizopus oligosporus confers antifungal activity to transgenic tobacco (1997)
    Springer
    Plant cell reports 16 (1997), S. 439-443 
    Details
    ISSN: 1432-203X
    Keywords: Fungal chitinase ; Transgenic tobacco ; Antifungal activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have studied whether a chitinase involved in cell autolysis of a filamentous fungus,Rhizopus oligosporus, can operate as an antifungal defense system in tobacco. Thechi1 gene was introduced into tobacco by theAgrobacterium tumefaciens leaf disc system. Among 22 transgenic tobacco plants, 2 were selected and their individual homozygous progeny, Tch1-1 and Tch2-1, were studied. Chitinase activity in the extracts of young leaves from Tch1-1 or Tch2-1, in which thechi1 gene product was detected by Western blot analysis, was three- to four-fold higher than that from the control plants. A fungal infection assay on the leaves infected with the discomycete pathogensSclerotinia sclerotiorum andBotrytis cinerea revealed that the symptoms observed with these two were remarkably suppressed as compared with the control leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092762
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    A fungal chitinase gene from Rhizopus oligosporus confers antifungal activity to transgenic tobacco (1997)
    Springer
    Plant cell reports 16 (1997), S. 439-443 
    Details
    ISSN: 1432-203X
    Keywords: Key words Fungal chitinase ; Transgenic tobacco ; Antifungal activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have studied whether a chitinase involved in cell autolysis of a filamentous fungus, Rhizopus oligosporus, can operate as an antifungal defense system in tobacco. The chi1 gene was introduced into tobacco by the Agrobacterium tumefaciens leaf disc system. Among 22 transgenic tobacco plants, 2 were selected and their individual homozygous progeny, Tch1-1 and Tch2-1, were studied. Chitinase activity in the extracts of young leaves from Tch1-1 or Tch2-1, in which the chi1 gene product was detected by Western blot analysis, was three- to four-fold higher than that from the control plants. A fungal infection assay on the leaves infected with the discomycete pathogens Sclerotinia sclerotiorum and Botrytis cinerea revealed that the symptoms observed with these two were remarkably suppressed as compared with the control leaves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092762
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Fibrillary inclusions in neoplastic and fetal acinar cells of the pancreas (1996)
    Springer
    Virchows Archiv 428 (1996), S. 261-266 
    Details
    ISSN: 1432-2307
    Keywords: Acinar cell carcinoma ; Fetal pancreas ; Electron microscopy ; Fibrillary inclusion ; Rough endoplasmic reticulum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a case of pancreatic acinar cell carcinoma which contained a large number of pleomorphic inclusions with fibrillary internal structures and mature zymogen granules. To clarify the significance of fibrillary inclusions in the differentiation of acinar cells of the pancreas, we further investigated fetal pancreases (gestational weeks 16, 17, 19, 20 and 28). We found two types of inclusions: type A, corresponding to fibrillary inclusion of neoplastic acinar cells, was observed only in a 19-week fetus; type B showed a homogeneous density similar to that of zymogen granules. Type B was observed in all the fetuses after the 17th gestational week. Although the type A inclusion might be generated throught a different mechanism than the type B inclusion, the appearance of a large number of fibrillary inclusions in neoplastic acinar cells may represent a transient form of zymogen granule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196699
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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