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  • 1
    ISSN: 1572-9605
    Keywords: YBCO ; Cooper pair ; thin films ; ultrafast phenomena
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract The transient photoimpedance response of epitaxial YBa2Cu3O7−δ thin films has been studied using 100 fs Ti:Sapphire laser pulse excitation. Both temperature and photon energy dependence of the fast optical response signal (whose temperature dependence can be explained by a kinetic inductance model involving Cooper pair breaking) were studied. The pair breaking rate is strongly photon energy-dependent, with a resonance around 1.5 eV with a width of only 100 meV, which is very surprising given the strong electron correlation in this metallic system and may be explained in terms of the stripe phase model.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-203X
    Keywords: Key words Chlorophyll fluorescence ; Kanamycin resistance screening ; Tobacco ; Arabidopsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The applicability of a chlorophyll fluorescence assay for kanamycin (Km) resistance screening in transgenic tobacco (Nicotiana tabacum) and Arabidopsis thaliana plants was investigated. In wild-type leaves incubated in the presence of 200 mg/l Km, a decrease in maximum variable fluorescence ((Fv)m) and a significant increase in constant fluorescence (Fo) were observed. Using (Fv)m/Fo as a screening parameter, we were able to distinguish Km-treated samples from untreated samples within 4 days. This parameter was applied to Km resistance screening using tobacco plants transformed with the nptII gene via Agrobacterium. Among 74 shoots selected on medium containing 200 mg/l Km, 37 plants were scored as Km sensitive by the chlorophyll fluorescence assay. These 74 scorings proved to be accurate, as reconfirmed by (1) polymerase chain reaction amplification of the transgene, (2) enzymatic assay of neomycin phosphotransferase and (3) leaf disc assay. Using the chlorophyll fluorescence assay, we could also screen 3-week old Arabidopsis plants carrying the nptII gene. These results clearly demonstrate the reliability and efficiency of this nondestructive assay for Km resistance screening of transgenic plants.
    Type of Medium: Electronic Resource
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