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  • 1995-1999  (4)
  • Self-incompatibility  (2)
  • Bcl-2  (1)
  • Brassica campestris  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Visualization of a self-incompatibility gene in Brassica campestris L. by multicolor FISH (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 751-757 
    Details
    ISSN: 1432-2242
    Keywords: Key words Brassica campestris ; Multicolor ; FISH ; Self-incompatibility ; S-glycoprotein (SLG) gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The physical localization of the S-glycoprotein (SLG) locus in the chromosome of Brassica campestris L. ‘pekinensis’ cv ‘Kukai’ was visualized by multi-color fluorescent in situ hybridization (McFISH). ‘Kukai’, which is an F1 hybrid between two parental lines, T-17 and T-18, has two SLG genes from both T-17 and T-18. In this study, a 1.3-kb DNA fragment was amplified from the genomic DNA of T-17 by PCR using a set of primers specific to the class-I SLG. From the genomic DNA of T-18, no DNA fragment was amplified using these primers. In the genomic Southern hybridization, a cloned PCR product hybridized with the genomic DNA of T-17 or F1 but not with that of T-18. The PCR product had a sequence homology of approximately, 85% to another class-I SLG gene, SLG-9. Therefore, the PCR product from T-17 was named SLG-17, as it is thought to be a member of the class-I SLG. Using SLG-17 as the probe, FISH was carried out to visualize the position of the SLG locus. McFISH was also carried out simultaneously using the SLG-17 and SLG-9 genes as probes. The SLG-17 gene was detected as a doublet signal at the interstitial region close to the end of a small chromosome, with the signal site being identical to that of SLG-9. Therefore, it is concluded that the SLG-17 gene is localized at the interstitial region close to the end of the chromosome derived from T-17 in Brassica campestris L. ‘pekinensis’ cv ‘Kukai’.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050798
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Centromeric localization of an S-RNase gene in Petunia hybrida Vilm. (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 391-397 
    Details
    ISSN: 1432-2242
    Keywords: Key words Centromeric specific repetitive sequence ; FISH ; Petunia hybrida ; Self-incompatibility ; S-RNase gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  S-RNase has been identified to be an S-allele-specific stylar determinant contributing to the self-incompatibility response in Solanaceae. In order to examine the physical location of the S-RNase gene, multi-color fluorescence in situ hybridization (FISH) using the S B1 -RNase cDNA probe and ribosomal RNA gene (rDNA) probe was performed on an S B1 S B2 heterozygote of Petunia hybrida. The S B1 -RNase gene was detected as a doublet signal close to the centromere of chromosome III. Next, we performed FISH using a large genome probe prepared from a λSB1–311 clone (20 kb) which contains the S B1 -RNase gene and its 3´ flanking region. This probe hybridized to the centromeric regions of all P. hybrida chromosomes. Sequence analysis of the λSB1–311 clone revealed the presence of a repetitive sequence consisting of a novel 666 bp unit sequence. A subclone (pBS-SB1B5) containing this unit sequence also hybridized to all of the centromeric regions, confirming that this unit is the centromeric specific repetitive sequence. These data suggested that the S B1 -RNase gene is located very close to (within a distance of 12 kb from) the centromeric-specific repetitive sequence. Likewise, the pBS-SB1B5 probe hybridized to the centromeric regions of all chromosomes in P. littoralis, another Petunia species. However, the probe did not hybridize to the centromere of the chromosomes from other species in Solanaceae. These results suggested that this centromeric repetitive sequence might be a genus-specific one.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051249
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    BAG-1 inhibits p53-induced but not apoptin-induced apoptosis (1997)
    Springer
    Apoptosis 2 (1997), S. 395-402 
    Details
    ISSN: 1573-675X
    Keywords: Apoptosis ; apoptin ; BAG-1 ; Bcl-2 ; p53 ; programmed cell death
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract BAG-1 has been identified as a Bcl-2-binding protein that inhibits apoptosis, either alone or in co-operation with Bcl-2. Here we show that BAG-1 inhibits p53- induced apoptosis in the human tumour cell line Saos-2. In contrast, BAG-1 was unable to inhibit the p53-independent pathway induced by apoptin, an apoptosis-inducing protein derived from chicken anaemia virus. Whereas BAG-1 seemed to co-operate with Bcl-2 to repress p53-induced apoptosis, co-expression of these proteins had no inhibitory effect on apoptin-induced apoptosis. Moreover, Bcl-2, and to some extent also BAG-1, paradoxically enhanced the apoptotic activity of apoptin. These results demonstrate that p53 and apoptin induce apoptosis through independent pathways, which are differentially regulated by BAG-1 and Bcl-2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026409808732
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Three members of the S multigene family are linked to the S locus of Brassica (1997)
    Springer
    Molecular genetics and genomics 256 (1997), S. 257-264 
    Details
    ISSN: 1617-4623
    Keywords: Key words Self-incompatibility ; Brassica campestris ; Receptor-like kinase ; S multigene family ; Gene cluster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two self-incompatibility genes in Brassica, SLG and SRK (SLG encodes a glycoprotein; SRK encodes a receptor-like kinase), are included in the S multigene family. Products of members of the S multigene family have an SLG-like domain (S domain) in common, which may function as a receptor. In this study, three clustered members of the S multigene family, BcRK1, BcRL1 and BcSL1, were characterized. BcRK1 is a putative functional receptor kinase gene expressed in leaves, flower buds and stigmas, while BcRL1 and BcSL1 are considered to be pseudogenes because deletions causing frameshifts were identified in these sequences. Sequence and expression pattern of BcRK1 were most similar to those of the Arabidopsis receptor-like kinase gene ARK1, indicating that BcRK1 might have a function similar to that of ARK1, in processes such as cell expansion or plant growth. Interestingly, the region containing BcRK1, BcRL1 and BcSL1 is genetically linked to the S locus and the physical distance between SLG, SRK and the three S-related genes was estimated to be less than 610 kb. Thus the genes associated with self-incompatibility exist within a cluster of S-like genes in the genome of Brassica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050568
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    Paper (German National Licenses)
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