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  • 1
    ISSN: 1432-1912
    Keywords: Key words 5-Hydroxytryptamine (5-HT ; serotonin) ; 5-HT1D receptors ; 5-HT1Dβ receptors ; Endothelial cells ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pharmacological evidence has suggested the presence of 5-hydroxytryptamine (5-HT, serotonin), 5-HT1D receptors on endothelial cells but these receptors have never been identified unambiguously on this type of cells. We now report that human umbilical vein endothelial cells (HUVEC) express 5-HT1D receptors coupled to inhibition of cyclic AMP formation. 5-HT and the 5-HT1D receptor agonists 5-carboxamidotryptamine (5-CT) and sumatriptan were approximately equipotent at inhibiting forskolin-stimulated cyclic AMP accumulation in HUVEC (mean pEC50 7.6–8.2, maximal effect 30% inhibition). The 5-HT1A receptor agonist, 8-OH-DPAT was clearly less potent (pEC50 6.2) and less efficacious. The selective 5-HT1D receptor antagonist, GR127935 (1 nM) markedly inhibited the effect of 5-HT (apparent pKB 10.8). Reverse transcription-polymerase chain reaction analysis showed the mRNA for 5-HT1Dβ receptors to be expressed in HUVEC. These results demonstrate the presence of functional 5-HT1D receptors and the expression of 5-HT1Dβ receptor mRNA in HUVEC. They support the involvement of 5-HT1Dβ receptors in endothelial-mediated responses to 5-HT.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1912
    Keywords: 5-Hydroxytryptamine (5-HT, serotonin) ; 5-HT1D receptors ; 5-HT1Dβ receptors ; Endothelial cells ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pharmacological evidence has suggested the presence of 5-hydroxytryptamine (5-HT, serotonin), 5-HT1D receptors on endothelial cells but these receptors have never been identified unamiguously on this type of cells. We now report that human umbilical vein endothelial cells (HUVEC) express 5-HT1D receptors coupled to inhibitiion of cyclic AMP formatin. 5-HT and the 5-HT1D receptor agaonists 5-carboxamidotryptamine (5-CT) and sumatriptan were approximately equipotent at inhibiting forskolin-stimulated cyclic AMP accumulation in HUVEC (mean pEC50 7.6–8.2, maximal effect 30% inhibition). The 5-HT1A receptor agonist, 8-OH-DPAT was clearly less potent (pEC50 6.2) and less efficacious. The selective 5-HT1D receptor antagonist, GR127935 (1 nM) markedly inhibited the effct pf 5-HT (apparent pKB 10.8). Reverse transcription-polymerase chain reaction analysis showed the mRNA for 5-HT1Dβ receptors to be expressed in HUVEC. These results demonstrate the presence of functional 5-HT1D receptors and the expression of 5-HT1Dβreceptor mRNA in HUVEC. They support he involvement of 5-HT1Dβ receptors in endothelial-mediated responses to 5-HT.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 27 (1996), S. 287-296 
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: The mechanical properties of polycristalline materials are depent on the type, the combination and the orientation of the elements in the micro structure. By means of the method of Visioplasticity assumptions are currently realized to analyse kinematic parameters inside of single grains and grain groups. Strength of the free surface of the specimen will be rough by its plastical deformation, it was difficult to register the increments of deformation of the grid. By further development of a method of picture analysis it is also possible to reproduce a 3d-surface by utilization of single light microscopical pictures.
    Notes: Die mechanischen Eigenschaften polykristalliner Werkstoffe sind von der Art der Mikrostrukturelemente, deren Zusammenwirken und Orientierung hauptsächlich abhängig. Mittels der Methode der Visioplastizität werden gegenwärtig Voraussetzungen geschaffen, die die Analyse kinematischer Feldkenngrößen innerhalb einzelner Strukturelemente (Kristallite) und Strukturverbände gestattet. Aufgrund der verformungsbedingten Aufrauhung der freien Probenoberfläche traten Probleme bei der Aufnahme der Rasterverformungen auf. Über die Weiterentwicklung eines Verfahrens der Bildverarbeitung wurde es möglich, 3D-Oberflächenstrukturen anhand der Auswertung lichtmikroskopischer Einzelbildaufnahmen abzubilden.
    Additional Material: 25 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 70 (1998), S. 560-566 
    ISSN: 0009-286X
    Keywords: Chemistry ; Industrial Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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