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  • 1
    Electronic Resource
    Electronic Resource
    Immunohistochemical study of the colonic muscle and innervation in idiopathic chronic constipation (1995)
    Springer
    Diseases of the colon & rectum 38 (1995), S. 509-513 
    Details
    ISSN: 1530-0358
    Keywords: Colon ; Innervation ; Nerves ; Muscle ; Immunohistochemistry ; Constipation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract PURPOSE: This study was designed to investigate neural and muscular features of the colonic wall in patients with severe idiopathic constipation. METHODS: By using quantitative immunohistochemistry, resected specimens from 14 patients with idiopathic chronic constipation and 17 nonobstructed cancer controls were studied. RESULTS: Routine histology revealed no significant histologic abnormality throughout the colon apart from four cases of melanosis coli. Ratio of the thickness of circular to longitudinal muscle was significantly lower in the left colon in constipated subjects. The myenteric plexus appeared morphologically normal in all subjects. S-100 protein, which stains neuronal supporting tissues, demonstrated an increase in the proportion of neural tissue in the myenteric plexus. There was an increased number of PGP-9.5 immunoreactive nerve fibers in the muscularis propria in constipated patients, and this was significantly higher in the ascending and descending colon. CONCLUSION: Intractably constipated patients have alterations in the neural composition of the colonic myenteric plexus and innervation of the circular muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02148851
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    NADH oxidase ofThermus thermophilus HB8 overproduced fromEscherichia coli (1995)
    Springer
    Protoplasma 184 (1995), S. 104-110 
    Details
    ISSN: 1615-6102
    Keywords: NADH oxidase ; Thermostable flavoprotein ; Affinity chromatography ; Codon usage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An NADH oxidase purified from the extreme thermophileThermus thermophilus HB8 is a monomeric flavoprotein with a 1 ∶ 1 ratio of flavin-adenine dinucleotide (FAD) to the polypeptide chain. It catalyzes in vitro the oxidation of reduced NADH or NADPH with the formation of H2O2. The gene encoding the NADH oxidase fromT. thermophilus HB8 was cloned, and its nucleotide sequence was determined. The molecular mass of 22,749 Da, as deduced from thenox gene, agrees with that of the purified NADH oxidase fromT. thermophilus HB8, as estimated by mass spectrometry. Thenox gene does not contain a GX4GK consensus sequence typical for nucleotide binding proteins. Thenox gene was overexpressed inEscherichia coli, and a protocol for the rapid purification of theE. coli-borneT. thermophilus NADH oxidase or its His6-tagged analogue was developed by using thermal denaturation step and affinity chromatography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276906
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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