Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 1995-1999  (6)
  • Degraded DNA  (2)
  • Eye morphogenesis  (2)
  • cytotoxicity  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Evaluation of new primers for CSF1PO (1997)
    Springer
    International journal of legal medicine 110 (1997), S. 36-38 
    Details
    ISSN: 1437-1596
    Keywords: Key words CSF1PO ; STR polymorphism ; Forensic identification ; Degraded DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract We describe new primers for the detection of the STR polymorphism at the CSF1PO locus. These primers have been designed to produce shorter amplicons (150– 182 bp) than the primers in standard use (295–327 bp). The reliability of the new primers for CSF1PO typing has been demonstrated by testing on known samples and by sequence analysis. These primers are superior to the original primers with regard to electrophoretic resolution and utility for typing of severely degraded DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00007698
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Evaluation of new primers for CSF1PO (1997)
    Springer
    International journal of legal medicine 110 (1997), S. 36-38 
    Details
    ISSN: 1437-1596
    Keywords: CSF1PO ; STR polymorphism ; Forensic identification ; Degraded DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract We describe new primers for the detection of the STR polymorphism at the CSF1PO locus. These primers have been designed to produce shorter amplicons (150–182 bp) than the primers in standard use (295–327 bp). The reliability of the new primers for CSF1PO typing has been demonstrated by testing on known samples and by sequence analysis. These primers are superior to the original primers with regard to electrophoretic resolution and utility for typing of severely degraded DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02441025
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Weakened cellular scavenging activity against oxidative stress in diabetes mellitus: regulation of glutathione synthesis and efflux (1995)
    Springer
    Diabetologia 38 (1995), S. 201-210 
    Details
    ISSN: 1432-0428
    Keywords: Glutathione ; γ-glutamylcysteine synthetase ; thiol transport ; erythrocytes ; cytotoxicity ; non-insulin-dependent diabetes mellitus ; K562 cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glutathione functions to scavenge oxidants or xenobiotics by covalently binding them and transporting the resulting metabolites through an adenosine 5′-triphosphate-dependent transport system. It has been reported that the intracellular concentration of glutathione decreases in diabetes mellitus. In order to elucidate the physiological significance and the regulation of anti-oxidants in diabetic patients, changes in the activity of the glutathione-synthesizing enzyme, γ-glutamylcysteine synthetase, and transport of thiol [S-(2,4-dinitrophenyl)glutathione] were studied in erythrocytes from patients with non-insulin-dependent diabetes and K562 cells cultured with 27 mmol/l glucose for 7 days. The activity of γ-glutamylcysteine synthetase, the concentration of glutathione, and the thiol transport were 77%, 77% and 69%, respectively in erythrocytes from diabetic patients compared to normal control subjects. Treatment of patients with an antidiabetic agent for 6 months resulted in the restoration of γ-glutamylcysteine synthetase activity, the concentration of glutathione, and the thiol transport. A similar impairment of glutathione metabolism was observed in K562 cells with high glucose levels. The cytotoxicity by a xenobiotic (1-chloro-2,4-dinitrobenzene) was higher in K562 cells with high glucose than in control subjects (50% of inhibitory concentration. 300±24 Μmol/l vs 840±29 Μmol/l, p〈0.01). Expression of γ-glutamylcysteine synthetase protein was augmented in K562 cells with high glucose, while enzymatic activity and expression of mRNA were lower than those in the control subjects. These results suggest that inactivation of glutathione synthesis and thiol transport in diabetic patients increases the sensitivity of the cells to oxidative stresses, and these changes may lead to the development of some complications in diabetes mellitus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400095
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Weakened cellular scavenging activity against oxidative stress in diabetes mellitus: regulation of glutathione synthesis and efflux (1995)
    Springer
    Diabetologia 38 (1995), S. 201-210 
    Details
    ISSN: 1432-0428
    Keywords: Key words Glutathione ; γ-glutamylcysteine synthetase ; thiol transport ; erythrocytes ; cytotoxicity ; non-insulin-dependent diabetes mellitus ; K562 cells.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glutathione functions to scavenge oxidants or xenobiotics by covalently binding them and transporting the resulting metabolites through an adenosine 5′-triphosphate-dependent transport system. It has been reported that the intracellular concentration of glutathione decreases in diabetes mellitus. In order to elucidate the physiological significance and the regulation of anti-oxidants in diabetic patients, changes in the activity of the glutathione-synthesizing enzyme, γ-glutamylcysteine synthetase, and transport of thiol [S-(2,4-dinitrophenyl)gluta- thione] were studied in erythrocytes from patients with non-insulin-dependent diabetes and K562 cells cultured with 27 mmol/l glucose for 7 days. The activity of γ-glutamylcysteine synthetase, the concentration of glutathione, and the thiol transport were 77 %, 77 % and 69 %, respectively in erythrocytes from diabetic patients compared to normal control subjects. Treatment of patients with an antidiabetic agent for 6 months resulted in the restoration of γ-glutamylcysteine synthetase activity, the concentration of glutathione, and the thiol transport. A similar impairment of glutathione metabolism was observed in K562 cells with high glucose levels. The cytotoxicity by a xenobiotic (1-chloro-2,4-dinitrobenzene) was higher in K562 cells with high glucose than in con- trol subjects (50 % of inhibitory concentration; 300 ± 24 μmol/l vs 840 ± 29 μmol/l, p 〈 0.01). Expression of γ-glutamylcysteine synthetase protein was augmented in K562 cells with high glucose, while enzymatic activity and expression of mRNA were lower than those in the control subjects. These results suggest that inactivation of glutathione synthesis and thiol transport in diabetic patients increases the sensitivity of the cells to oxidative stresses, and these changes may lead to the development of some complications in diabetes mellitus. [Diabetologia (1995) 38: 201–210]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400095
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    An eye imaginal disc-specific transcriptional enhancer in the long terminal repeat of the tom retrotransposon is responsible for eye morphology mutations of Drosophila ananassae (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 161-166 
    Details
    ISSN: 1617-4623
    Keywords: Key words Drosophila ananassae ; Om mutations ; tom element ; Retrotransposon ; Eye morphogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Optic morphology (Om) mutations of Drosophila ananassae are semidominant, neomorphic and nonpleiotropic, map to at least 22 loci scattered throughout the genome, and are associated with the insertion of the tom retrotransposon. Molecular and genetic analyses have revealed that eye morphology defects of Om mutants are caused by the ectopic or excessive expression of Om genes in the eye imaginal discs of third instar larvae. It is therefore assumed that the tom element carries tissue-specific gene regulatory sequences which enhance expression of the Om genes. In the present study, we examined whether or not the long terminal repeats (LTR) of the tom element contain such an eye imaginal disc-specific enhancer, using D. melanogaster transformants containing a lacZ gene ligated to the tom LTR. Analyses of lacZ gene expression in the eye imaginal discs of third instar larvae of 18 independently established transformant lines showed that the tom LTR was capable of enhancing lacZ expression in all the transformant lines, but the degree of enhancement varied between lines. In addition, the effect of the tom LTR lacZ gene evidently changed when the tom LTR construct was relocated to different chromosomal positions. On the basis of these findings, it is hypothesized that ectopic and excessive expression of the Om genes in the eye imaginal discs is induced by an eye imaginal disc-specific enhancer present in the tom LTR, the effect of which may be subject to chromosomal position effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172914
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    An eye imaginal disc-specific transcriptional enhancer in the long terminal repeat of thetom retrotransposon is responsible for eye morphology mutations ofDrosophila ananassae (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 161-166 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila ananassae ; Om mutations ; tom element ; Retrotransposon ; Eye morphogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Optic morphology (Om) mutations ofDrosophila ananassae are semidominant, neomorphic and nonpleiotropic, map to at least 22 loci scattered throughout the genome, and are associated with the insertion of thetom retrotransposon. Molecular and genetic analyses have revealed that eye morphology defects ofOm mutants are caused by the ectopic or excessive expression ofOm genes in the eye imaginal discs of third instar larvae. It is therefore assumed that thetom element carries tissue-specific gene regulatory sequences which enhance expression of theOm genes. In the present study, we examined whether or not the long terminal repeats (LTR) of thetom element contain such an eye imaginal disc-specific enhancer, usingD. melanogaster transformants containing alacZ gene ligated to thetom LTR. Analyses oflacZ gene expression in the eye imaginal discs of third instar larvae of 18 independently established transformant lines showed that thetom LTR was capable of enhancinglacZ expression in all the transformant lines, but the degree of enhancement varied between lines. In addition, the effect of thetom LTRlacZ gene evidently changed when thetom LTR construct was relocated to different chromosomal positions. On the basis of these findings, it is hypothesized that ectopic and excessive expression of theOm genes in the eye imaginal discs is induced by an eye imaginal disc-specific enhancer present in thetom LTR, the effect of which may be subject to chromosomal position effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172914
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...