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  • 1995-1999  (2)
  • Keywords Glucose-regulated gene  (1)
  • Lateral and medial vestibulospinal tracts  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Identification of genes regulated by glucose in a pancreatic beta-cell line by a new method for subtraction of mRNA (1996)
    Springer
    Diabetologia 39 (1996), S. 1293-1298 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Glucose-regulated gene ; cDNA subtraction ; gene expression ; polymerase chain reaction ; expressed sequence tag.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Various genes are differentially expressed in cells during cell differentiation, development, aging, and in pathological conditions. To identify and isolate the genes that are specifically and differentially expressed in cells, we established a ligation-mediated polymerase chain reaction (PCR)-based method for cDNA subtraction. As this method is PCR-based, when target genes are expressed at high levels relative to the driver (a control pool for subtraction), even a small amount of target genes can be amplified. By this newly developed PCR-based subtraction method, a set of genes regulated by glucose were identified in a mouse insulinoma cell line. This PCR-based and non-radioactive subtraction method will be a powerful tool for identification of novel genes, specifically and differentally expressed in cells. [Diabetologia (1996) 39: 1293–1298]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250050572
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Properties of utricular nerve-activated vestibulospinal neurons in cats (1996)
    Springer
    Experimental brain research 112 (1996), S. 197-202 
    Details
    ISSN: 1432-1106
    Keywords: Utricular nerve ; Vestibulospinal neuron ; Lateral and medial vestibulospinal tracts ; Vestibular nuclei ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The axonal pathway, conduction velocities, and locations of the cell bodies of utricular nerve-activated vestibulospinal neurons were studied in decerebrated or anesthetized cats using the collision test of orthodromic and antidromic spikes. For orthodromic stimulation, bipolar tungsten electrodes were placed on the utricular nerve and the other vestibular nerve branches were transected. Monopolar tungsten electrodes were positioned on both sides of the upper cervical segments (C2–4), caudal end of the cervical enlargement (C7-T1), and from the lower thoracic to the upper lumbar segments (T12-L3) and were used for antidromic stimulation of the spinal cord. Another monopolar electrode was also placed in the oculomotor nucleus to study whether utricular nerve-activated vestibulospinal neurons have ascending branches to the oculomotor nucleus. Of the 173 vestibular neurons orthodromically activated by the stimulation of the utricular nerve, 46 were second-order vestibulospinal neurons and 5 were third-order neurons. The majority of the utricular nerve-activated vestibulospinal neurons were located in the rostral part of the descending vestibular nucleus and the caudal part of the ventral lateral nucleus. Seventy-three percent of the utricular nerve-activated vestibulospinal neurons descended through the ipsilateral lateral vestibulospinal tract. Approximately 80% of these neurons reached the cervicothoracic junction, but a few reached the upper lumbar spinal cord. Twenty-seven percent of the utricular nerve-activated vestibulospinal neurons descended through the medial vestibulospinal tract or the contralateral vestibulospinal tracts. Those axons terminated mainly in the upper cervical segments. Almost none of the utricular nerve-activated vestibular neurons had ascending branches to the oculomotor nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227638
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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