ISSN:
1573-8221
Keywords:
α-1-antitrypsin
;
transgenic animals
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract A genetic-engineering construction is developed to provide expression of the full-size cDNA of human α-1-antitrypsin controlled by the promoter elements of the murine metallothionein-1 gene. Transgenic animals were obtained by microinjection of pAT-MT plasmid into fertilized rat oocytes. The genome of such animals contains the sequences of human α-1-antitrypsin-cDNA revealed by blot- and dot-hybridization. Integrated human α-1-antitrypsin-cDNA copies are found with a high frequency (9 out of 22 animals) in the second-generation offspring obtained after crossings of transgenic animals. A protein with antigenic specificity of human a-1-antitrypsin is found in the serum of transgenic rats by the immunoblotting method.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02444673
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