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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 133 (1995), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: BP180 is a 180kDa hemidesmosomal protein recognized by bullous pemphigoid (BP) and pemphigoid gestationis (PG) autoantibodies. Recent cloning and sequence analysis performed by our laboratory have revealed that BP180 is a transmembrane protein with a long extracellular collagen-like region. A rabbit polyclonal antibody has been generated against a recombinant protein, designated GST-NΔ1. containing a segment of the BP180 ectodomain. The resulting antiserum. RNΔ1 A, was shown to specifically react with BP180 on immunoblot, and labelled the extracellular region of the epidermal hemidesmosome on immunoelectron microscopy. A panel of normal and neoplastic human tissues were analysed by indirect immunofluorescence (IF) and RNΔ1A, to determine the distribution of BP180. A total of nine basal cell carcinomas (BCCs) and four squamous cell carcinomas (SCCs) of the skin were also studied. Intense IF staining was seen along the basement membrane zone (BMZ) of the epidermis, hair follicles, and the periphery of sebaceous gland lobules. The sebaceous lobules showed more intense staining in areas close to the duct, The epithelial BMZ of the following tissues also reacted with RNΔ1A: cornea, ocular conjunctiva, buccal mucosa. upper oesophagus, placenta (amnion placentum). umbilical cord and transitional epithelium of the bladder, The epithelium of the jejunum and ovary tailed to react with RNΔ1A, Staining of the BCCs and SCCs was variable. Five of six nodular BCCs showed some anti-BP180 staining at the tumour-stromal interface, although the level of staining was less intense than that observed in the overlying normal epidermis. All three morphoeic BCCs analysed in this investigation did not show any staining with RNΔ1A. Three of four SCCs showed weak staining at the tumour-stromal interface. Thus, the tissue distribution of BP180 paralleled that of hemidesmosomes, and expression of this protein was found to be decreased or absent in cutaneous neoplasms.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Neuroscience 18 (1995), S. 385-408 
    ISSN: 0147-006X
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Nephrology 1 (1995), S. 0 
    ISSN: 1440-1797
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary: Many programmes have attempted community education in organ donation. Too often these ‘preach to the converted’ or concentrate on special interest groups, thus failing to widely influence community perceptions of transplantation and organ donation.As 13–14 year old children appear sufficiently mature to be receptive to challenging ideas yet may not yet have developed fixed biases or opinions to impair concept assimilation, an educational package dealing with dialysis/transplantation and organ donation was tested in two evenly aged, socio-economic and sex matched student groups at this age level in a single co-educational school. One group only was exposed to a 3 h comprehensive course in renal structure and function, dialysis and transplantation. the two groups were assessed 1 year later by questionnaire to test the retention of information and, more importantly, to compare attitudes to organ donation.Statistically significant differences characterized the knowledge of and attitudes towards dialysis and transplantation between the two groups. In particular, the tutored group was much more likely to agree to organ donation by self (P〈0.009) or by relatives (P〈0.03) when compared to the untutored group.This study demonstrates that a short, simple education course for 13–14 year old students can favourably change their perceptions and attitudes towards organ donation. the wider application of similar courses may have a positive impact upon dialysis and transplantation awareness and, ultimately, on national donor rates, becoming a useful addition to national strategies to increase organ donation.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Suite 500, 5th Floor, 238 Main Street, Cambridge, Massachusetts 02142, USA : Blackwell Science Inc.
    International journal of gynecological cancer 5 (1995), S. 0 
    ISSN: 1525-1438
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Euthanasia means the active causation of death by a life-shortening medical act performed on a seriously ill patient who has a concrete expectation to die. The request for euthanasia has always existed. In medical ethics the value of the protection of human life and the value of respect for the desire to die in dignity are both standards of equal order. In the process of euthanasia the wish of the patient is the most important. It is the responsibility of the doctor in attendance to keep all the personnel involved very well informed about the wishes of the patient, to prevent difficulties in the process of decision making. One has to realize that the emotional burden assumed by the doctor and nurses is very important. They become involved in the process of taking leave but have to give priority to their task as caregivers. Euthanasia can take place only as a part of terminal care which is given with much effort and personal concern.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    World journal of surgery 21 (1997), S. 379-383 
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. In a prospective observational study including 34 patients with carcinoma of the ampulla of Vater, postoperative morbidity, mortality, and long-term survival were analyzed to determine the surgical procedure of choice. Surgically related postoperative complications were observed in 35.4% of patients after pancreatic resection. No patient died within the first 30 days postoperatively, and in-hospital mortality was 3%. Lymph node metastases were associated only with moderate or undifferentiated tumors larger than 0.6 cm in diameter that infiltrated beyond the ampulla of Vater. The median follow-up time was 4.3 years. The 5-year survival rate for the 31 patients undergoing radical resection was 62.7%. Multivariate analysis (including the covariates depth of tumor infiltration, lymph node metastases, and the ratio of metastatic to dissected lymph nodes) demonstrated that only this ratio exerted an independent influence on the prognosis ( p = 0.001). The present series demonstrates that radical resection of ampullary cancer is the procedure of choice even in elderly patients. The most important factor influencing the survival rate is the extent of the lymph node dissection. The histopathologic investigation of our pancreatoduodenectomy specimens demonstrates clearly that local excision of ampullary cancer may be indicated only in high risk patients with a pT1, well differentiated tumor smaller than 0.6 cm in diameter.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Knochentumoren ; Chondrosarkom ; Osteosarkom ; Zytogenetik ; DNA-Zytometrie ; Ploidie ; Key words Bone neoplasms ; Chondrosarcoma ; Osteosarcoma ; Cytogenetics ; DNA-cytometry ; Ploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary 10 chondrosarcomas and 10 osteosarcomas were examined using cytogenetics and DNA-image-cytometry. Cytogenetically 6 of 10 chondrosarcomas and 4 of 10 osteosarcomas showed hyperdiploid tumorcells. By DNA-cytometry in 8 of 10 chondrosarcomas and 9 of 10 osteosarcomas hyperdiploid tumorcells resp. hyperdiploid stemlines were detected. This discrepancy reflects an in-vitro-selection depending on the different entities. In 7 aneuploid clones of chondrosarcomas the chromosomal ploidy was calculated using the relative length of the chromosomes and compared with the DNA-ploidy of the native tumor. There was a close relation between both parameters of nuclear DNA-content. The interpretation of cytogenetic results is improved using a combination of karyotypic and DNA-cytometric examination. This is particularly important for the search for relations between numeric chromosomal aberrations and morphological parameters (grading).
    Notes: Zusammenfassung 10 Chondrosarkome und 10 Osteosarkome wurden tumorzytogenetisch und DNA-zytometrisch untersucht. Das Karyogramm erbrachte bei 6 von 10 Chondrosarkomen und bei 4 von 10 Osteosarkomen den Nachweis hyperdiploider Tumorzellklone. DNA-zytometrisch wurden am nativen Tumormaterial jedoch bei 8 von 10 Chondrosarkomen und bei 9 von 10 Osteosarkomen hyperdiploide Tumorzellen, häufig in Form eigenständiger hyperdiploider Stammlinien nachgewiesen. Diese Diskrepanz ist Ausdruck einer offenbar Entitäts-abhängigen In-vitro-Selektion. Bei insgesamt 7 aneuploiden Tumorzellklonen von Chondrosarkomen konnte die chromosomale Ploidie anhand der relativen Chromosomenlängen exakt errechnet und der zytometrisch bestimmten DNA-Ploidie gegenübergestellt werden, wobei sich eine sehr enge Abhängigkeit zwischen diesen beiden Parametern des nukleären DNA-Gehaltes ergab. Die Interpretation zytogenetischer Befunde bei Knochentumoren wird durch Kombination mit der DNA-Zytometrie verbessert. Dies ist besonders dann wichtig, wenn Zusammenhänge zwischen numerischen chromosomalen Aberrationen und morphologischen Parametern (z. B. Grading) dargestellt werden sollen.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Knochentumoren ; Solitäre Knochenzyste ; Aneurysmatische Knochenzyste ; Niedrigmalignes zentrales Osteosarkom ; Hochmalignes zentrales Osteosarkom ; DNA-Imagezytometrie ; DNA-Aneuploidie ; Key words Bone neoplasms ; Solitary bone cyst ; Aneurysmal bone cyst ; Low-grade central osteosarcoma ; High-grade central osteosarcoma ; DNA image cytometry ; DNA aneuploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary DNA cytometric investigations of intraosseous cystic lesions and osteoblastic tumors may be helpful in morphological diagnosis. The detection of a DNA-aneuploid stemline supports the diagnosis of a high-grade malignant bone neoplasm, even in small biopsies. Solitary and aneurysmal bone cysts have DNA-diploid cell populations. Low-grade central osteosarcomas may show single-cell aneuploidies of varying extent as well as a DNA-diploid stemline. Their presence may be a sign of genetic instability within the population of neoplastic cells before a DNA-aneuploid stemline is established and should prompt careful postoperative observation.
    Notes: Zusammenfassung DNA-zytometrische Untersuchungen intraossärer zystischer Läsionen und osteoblastischer Tumoren können die morphologische Diagnostik unterstützen. Der Nachweis von DNA-aneuploiden Tumorzellstammlinien trägt zur Sicherung der Diagnose eines hochmalignen Knochentumors auch an kleinen Biopsien bei. Solitäre und aneurysmatische Knochenzysten weisen DNA-diploide Zellpopulationen auf. Bei zentralen niedrigmalignen Osteosarkomen können neben einer diploiden Stammlinie Einzelzellaneuploidien unterschiedlichen Ausmaßes vorkommen. Ihr Auftreten kann bereits ein Hinweis auf eine genetische Instabilität innerhalb der Tumorzellpopulation sein, bevor es zur Ausprägung einer DNA-aneuploiden Stammlinie kommt und sollte bei differentialdiagnostisch schwierigen Fällen intraossärer Läsionen zu sorgfältiger Nachbeobachtung Anlaß sein.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1433-0385
    Keywords: Key words: Endoscopic adrenalectomy ; ASA classification. ; Schlüsselwörter: Endoskopische Adrenalektomie ; ASA-Klassifikation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Bei 20 Patienten wurden retrospektiv die Auswirkungen eines Pneumoretroperitoneums auf die intraoperative Ventilation und Hämodynamik während endoskopischer, retroperitonealer Adrenalektomie überprüft. Intraoperativ war ein Anstieg des systolischen Blutdrucks feststellbar [Median 180 mmHg (130–200)]. Hinsichtlich der Ventilation mußte das Atemminutenvolumen (AMV) intraoperativ im Median um 4,25 l (0,4–13) erhöht werden, um den als Folge der Anlage des Pneumoretroperitoneums ansteigenden arteriellen Kohlendioxid-Partialdruck zu senken. Die Anlage des Pneumoretroperitoneums führte zu keiner wesentlichen Komplikation, so daß uns die endoskopische, retroperitoneale Adrenalektomie auch bei Risikopatienten gerechtfertigt erscheint.
    Notes: Summary. In 20 patients the hemodynamic and ventilatory data during endoscopic retroperitoneal adrenalectomy were checked retrospectively to analyze the hemodynamic and ventilatory effects of a pneumoretroperitoneum. After insufflation of CO2 into the retroperitoneal space we observed an increase in systolic blood pressure [median 180 mm Hg ([130–200)] . Analyzing the ventilatory parameters we noted that the minute ventilation had to be invreased by a median value of 4.25 l (0,4–13) to reduce the increased carbon dioxide levels during CO2 insufflation. After insufflation of CO2 into the retroperitoneal space we observed no major complications. We therefore believe that retroperitoneal endoscopic adrenalectomy is also indicated in patients with cardiac or pulmonary diseases.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Der Chirurg 68 (1997), S. 801-805 
    ISSN: 1433-0385
    Keywords: Key words: ASA score ; Risk evaluation ; Complication ; Laparoscopic cholecystectomy ; Contraindication. ; Schlüsselwörter: ASA-Klassifikation ; Risikoeinschätzung ; Komplikationen ; Cholecystektomie ; laparoskopische ; Kontraindikation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Den Vorteilen der laparoskopischen Cholecystektomie steht eine erhöhte Gefährdung kardiopulmonal vorgeschädigter Patienten durch die hämodynamischen und respiratorischen Auswirkungen des Pneumoperitoneums gegenüber. In der Zeit von Juni 1990 bis Dezember 1995 wurden 19 Hochrisikopatienten (ASA IV) und 465 Patienten mit minderem Operationsrisiko (ASA I–III) laparoskopisch cholecystektomiert. Bei insgesamt 5 % (n = 24) an intraoperativen, kardiopulmonalen Komplikationen traten diese bei den Hochrisikopatienten mit 15,8 % (n = 3) vs. 4,5 % (n = 21) häufiger (p = 0,027) auf. Allgemeine, postoperative Komplikationen sahen wir bei 2,9 % (n = 14) aller Fälle, wobei ebenfalls die Patienten der Gruppe ASA IV vermehrt betroffen waren [15,8 % (n = 3) in der Gruppe ASA IV vs. 2,4 % (n = 11) in Gruppe ASA I–III (p 〈 0,001)]. Die Liegedauer war in der Hochrisikogruppe mit 7,6 ± 4,96 Tagen im Vergleich zu 4,8 ± 2,23 Tagen (Gruppe ASA I–III) verlängert (p = 0,022). Die Einstufung als Hochrisikopatient bedeutet insgesamt eine Erhöhung der perioperativen Komplikationsrate bei laparoskopischer Cholecystektomie, stellt jedoch keine grundsätzliche Kontraindikation für dieses Operationsverfahren dar.
    Notes: Summary. As opposed to the advantages of laparoscopic cholecystectomy, patients with cardiopulmonary impairment may be endangered by the haemodynamic and respiratory effects of the pneumoperitoneum. Between June 1990 and December 1995, laparoscopic cholecystectomies were performed in 19 high-risk patients (ASA IV) and 465 patients with a lower operative risk (ASA I–III). Out of 484 patients, 24 (5 %) suffered intraoperative cardiopulmonary complications. Three belonged to the high-risk group (15.8 %) and 21 to the lower risk groups (4.5 %). General postoperative complications occured in 14 cases (2.9 %), whereby patients of the ASA IV group were, again, concerned more often [15.8 % (n = 3) of ASA IV versus 2.4 % (n = 11) of ASA I–III]. The number of days spent in hospital was 7.6 ± 4.96 days in the high-risk group versus 4.8 ± 2.23 days in groups ASA I–III. The evaluation as a high-risk patient indicates an elevation of the perioperative rate of complications in laparoscopic cholecystectomy; however, it is not basically a contraindication for this operative method.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1433-0385
    Keywords: Key words: ASA score ; Risk evaluation ; Complications ; Laparoscopic cholecystectomy ; Contraindications. ; Schlüsselwörter: ASA-Klassifikation ; Risikoeinschätzung ; Komplikationen ; laparoskopische Cholecystektomie ; Kontraindikation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Die laparoskopischen Cholecystektomie bietet eine Reihe von Vorteilen, denen eine erhöhte Gefährdung kardiopulmonal vorgeschädigter Patienten durch die hämodynamischen und respiratorischen Auswirkungen des Pneumoperitoneums gegenübersteht. In der Zeit von Juni 1990 bis Dezember 1995 wurden 19 Hochrisikopatienten (ASA IV) laparoskopisch und 26 Patienten mit gleichem operativem Risiko (ASA IV) konventionell cholecystektomiert. Bei insgesamt 11,1 % (n = 5) an intraoperativen, kardiopulmonalen Komplikationen traten diese in beiden Gruppen annähernd gleichhäufig auf. Allgemeine, postoperative Komplikationen sahen wir in 33,3 % (n = 15) aller Fälle, wobei die konventionell operierten Patienten vermehrt betroffen waren [46,2 % (n = 12) vs. 15,8 % (n = 3), p = 0,03]. Die Liegedauer war in der Gruppe der offen operierten Patienten mit 11,6 ± 5,6 Tagen im Vergleich zu 7,6 ± 5 (Gruppe der laparoskopisch operierten Patienten) verlängert (p = 0,01). Die Einstufung als Hochrisikopatient bedeutet insgesamt eine Erhöhung der perioperativen Komplikationsrate, wobei bei gleichem intraoperativen Risiko die postoperative Komplikationsrate bei laparoskopischer Cholecystektomie niedriger ist.
    Notes: Summary. Laparoscopic cholecystectomy offers many advantages, but cardiopulmonary impaired patients may be endangered by the haemodynamic and respiratory effects of the pneumoperitoneum. Between June 1990 and December 1995, laparoscopic cholecystectomies were performed on 19 high-risk patients (ASA IV) and conventional cholecystectomies on 26 patients with the same operative risk (ASA IV). Out of 45 patients, 5 (11.1 %) suffered intraoperative cardiopulmonal complications. Three belonged to the group with laparoscopic cholecystectomy (15,8 %) and two to the group with open laparotomy (7.7 %). General postoperative complications occured in 15 cases (33.3 %), whereby patients of the conventional cholcystectomy group were concerned more often [46.2 % (n = 12) versus 15.8 % (n = 3), P = 0.03]. The number of days spent in hospital after open cholecystectomy was higher (P = 0.01) (11.6 ± 5.6 days in the laparotomy group versus 7.6 ± 5.0 days in the laparoscopy group). The classification as a high-risk patient indicates an elevation of the perioperative rate of complications in laparoscopic and open cholecystectomy, whereby the rate of postoperative complications is lower in the laparoscopic group.
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