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  • 1
    Electronic Resource
    Electronic Resource
    Changes in tracheal tube position during laparoscopic cholecystectomy (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Anaesthesia 51 (1996), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The distance from the carina to the tip of the tracheal tube was measured with a fibreoptic bronchoscope in 21 consecutive patients undergoing elective laparoscopic cholecystectomy. After placement of an Eschmann tracheal tube with a printed intubation guide mark at the vocal cords, the distance was 28 (15) [5–54]mm (mean (SD) [range]). The tube was then repositioned so that the distance was 34 (3) [30–40]mm from tip of the tube to the carina. After creation of pneumoperitoneum, the distance was significantly decreased to 26 (5) [17–35] mm (p 〈 0.005), which was not significantly altered by subsequent moving of the patient to the reverse Trendelenburg and left lateral tilt position. The maximum distance of tube migration was 8 (4) [0–15]mm. Four out of 21 patients would have been at risk of bronchial intubation after pneumoperitoneum if the tube had not been repositioned. Placement of the tube according to the guide mark is not recommended for laparoscopic cholecystectomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2044.1996.tb12609.x
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  • 2
    Electronic Resource
    Electronic Resource
    Are the two long prongs of the Mallinckrodt double-lumen endobronchial tube necessary? (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Anaesthesia 50 (1995), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2044.1995.tb06176.x
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  • 3
    Electronic Resource
    Electronic Resource
    Effects of nitrous oxide on haemodynamic and electroencephalographic responses induced by tetanic electrical stimulation during propofol anaesthesia (1999)
    Oxford : Blackwell Science Ltd
    Anaesthesia 54 (1999), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We studied the effect of nitrous oxide on haemodynamic and electroencephalographic responses caused by noxious stimulation during propofol anaesthesia. Thirty-four patients (ASA I–II) were anaesthetised with propofol 3 mgkg−1 and were randomly allocated to receive either 60% nitrous oxide in oxygen or 40% oxygen in air. Anaesthesia was maintained using propofol infusion of 10 mgkg−1.h−1 for the first 10 min, 8 mgkg−1.h−1 for the next 10 min and 6 mgkg−1.h−1 thereafter. Thirty minutes after the induction of anaesthesia, tetanic electrical stimulation (80 mA, 100 Hz) was applied to the ulnar nerve. Tetanic stimulation significantly increased blood pressure and heart rate in both groups (p 〈 0.005 or less), but did not induce any arousal pattern on the electroencephalograph. Nitrous oxide significantly attenuated the tetanic stimulation-induced increase in blood pressure (p 〈 0.05 or less), but not the heart rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2044.1999.00752.x
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  • 4
    Electronic Resource
    Electronic Resource
    Electroencephalographic arousal response during tracheal intubation and laryngeal mask airway insertion after induction of anaesthesia with propofol (1999)
    Oxford : Blackwell Science Ltd
    Anaesthesia 54 (1999), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Laryngoscopy and tracheal intubation, or insertion of a laryngeal mask airway may lead to an arousal response on the electroencephalogram. We studied whether more intense stimulation (laryngoscopy and tracheal intubation) causes a greater arousal response than less intense stimulation (laryngeal mask airway insertion). Thirty-four patients (ASA I–II) were anaesthetised with propofol 3 mg.kg−1, followed by vecuronium 0.15 mg.kg−1 and a propofol infusion of 10 mg.kg−1.h−1. Three minutes after induction of anaesthesia, either laryngoscopy and tracheal intubation (n = 18), or laryngeal mask airway insertion (n = 16) was performed. Laryngoscopy and tracheal intubation caused a significantly greater increase in blood pressure (but not heart rate) than laryngeal mask airway insertion (p 〈 0.05). Electroencephalogram responses were not different. More intense stimulation does not cause a greater arousal response during propofol anaesthesia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2044.1999.00944.x
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic uncoupling of the dsRNA-binding and RNA cleavage activities of the Escherichia coli endoribonuclease RNase III — the effect of dsRNA binding on gene expression (1998)
    Oxford BSL : Blackwell Science Ltd, UK
    Molecular microbiology 28 (1998), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: RNase III, a double-stranded RNA-specific endonuclease, is proposed to be one of Escherichia coli 's global regulators because of its ability to affect the expression of a large number of unrelated genes by influencing post-transcriptional control of mRNA stability or mRNA translational efficiency. Here, we describe the phenotypes of bacteria carrying point mutations in rnc, the gene encoding RNase III. The substrate recognition and RNA-processing properties of mutant proteins were analysed in vivo by measuring expression from known RNase III-modulated genes and in vitro from the proteins' binding and cleavage activities on known double-stranded RNA substrates. Our results show that although the point mutation rnc70 exhibited all the usual rnc null-like phenotypes, unlike other mutations, it was dominant over the wild-type allele. Multicopy expression of rnc70 could suppress a lethal phenotype of the wild-type rnc allele in a certain genetic background; it could also inhibit the RNase III-mediated activation of λN gene translation by competing for the RNA-binding site of the wild-type endonuclease. The mutant protein failed to cleave the standard RNase III substrates in vitro but exhibited an affinity for double-stranded RNA when passed through poly(rI):poly(rC) columns. Filter binding and gel-shift assays with purified Rnc70 showed that the mutant protein binds to known RNase III mRNA substrates in a site-specific manner. In vitro processing reactions with purified enzyme and labelled RNA showed that the in vivo dominant effect of the mutant enzyme over the wild-type was not necessarily caused by formation of mixed dimers. Thus, the rnc70 mutation generates a mutant RNase III with impaired endonucleolytic activity but without blocking its ability to recognize and bind double-stranded RNA substrates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1998.00828.x
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  • 6
    Electronic Resource
    Electronic Resource
    Severe 5-fluorouracil-related toxicity in a Japanese patient with dihydropyrimidine dehydrogenase deficiency (1999)
    Springer
    International journal of clinical oncology 4 (1999), S. 54-56 
    Details
    ISSN: 1437-7772
    Keywords: Key words Dihydropyrimidine dehydrogenase ; 5-Fluorouracil ; Gastric cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Dihydropyrimidine dehydrogenase (DPD) is the major enzyme involved in the catabolism of pyrimidines and fluoropyrimidines. Severe 5-fluorouracil (5-FU) toxicity has been reported in patients with DPD deficiency in Western countries. We now report a case of 5-FU-related severe toxicity in a Japanese patient with DPD deficiency. A-44 year-old woman with gastric cancer associated with peritoneal dissemination underwent palliative gastrectomy at the Tochigi Cancer Center. After surgery, she was treated with a combination of mitomycin C (12.5 mg/m2, bolus iv) and 5-FU (312.5 mg/m2 per day, continuous infusion). Eight days after commencement, the chemotherapy was discontinued because of severe gastrointestinal toxicity. On day 12 the patient developed a high fever with granulocytopenia (617.4 granulocytes/μl). DPD-activity in peripheral blood mononuclear cells (PBMC) was extremely low (up to 8.9 pmol/mg protein per min) and serum and urine uracil concentrations were high, at 225.2 ng/ml and 2334.2 ng/mg creatinine, respectively. To our knowledge, this appears to be the first case of 5-FU-related severe toxicity due to DPD deficiency reported in a Japanese patient.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101470050026
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  • 7
    Electronic Resource
    Electronic Resource
    Diets of the demersal fishes on the shelf off Iwate, northern Japan (1995)
    Springer
    Marine biology 123 (1995), S. 219-233 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diets of demersal fishes were determined on the shelf (ca. 130 m deep) off Iwate, Japan. Samples were taken in three different types of habitat, an artificial reef (AR) site, a natural reef (NR) site, and sandymud bottom (SB) site, from May 1987 to September 1991, mostly every two months. A total of 67 prey items were recognized in the stomachs of 45 predator fish species. The most important preys were the pelagic fishes Sardinops melanostictus and Engraulis japonicus, which comprised 37% wet wt of the overall stomach contents. The percentage of pelagic fishes was highest at AR site, where fish density was highest. The dominant ten species could be divided into five feeding types. The pelagic fish feeders Physiculus maximowiczi and Gadus macrocephalus fed mainly on S. melanostictus. The dietary breadth of P. maximowiczi was wide, while that of Gadus macrocephalus was narrow. The pelagic crustacean feeder Theragra chalcogramma mostly consumed Themisto japonica and euphausiids and showed the least dietary overlap with other fishes. Benthic fish feeders were Hemitripterus villosus and Liparis tanakai. The benthic crustacean feeders Alcichthys alcicornis and Hexagrammos otakii consumed benthic crustaceans as well as pelagic and benthic fishes and showed the largest dietary breadth. The benthic invertebrate feeders Gymnocanthus intermedius, Dexistes rikuzenius and Tanakius kitaharai fed mainly on polychaetes and benthic crustaceans. But Gymnocanthus intermedius consumed a significant proportion of pelagic fishes. Ontogenetic dietary shift was recognized for these fishes. Pelagic fishes were consumed more intensively by larger individuals, especially true of A. alcicornis, Theragra chalcogramma and Gadus macrocephalus. Predominancy of the two most adundant species, P. maximowiczi and A. alcicornis, may be supported by their wide dietary breadth and the significant proportion of pelagic fish in their diets. Interspecific dietary overlap was low in most cases suggesting that food resources were well partitioned, although some high overlap was observed among the pelagic fish feeders, A. alcicornis, and Gymnocanthus intermedius, and among the benthic invertebrate feeders. Interspecific competition seemed more likely in the benthic invertebrate feeders than in the pelagic fish feeders partly because of superabundance of the pelagic prey S. melanostictus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00353613
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  • 8
    Electronic Resource
    Electronic Resource
    Predation on Euphausia pacifica by demersal fishes: predation impact and influence of physical variability (1998)
    Springer
    Marine biology 132 (1998), S. 195-208 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The importance of Euphausia pacifica Hansen to the demersal fish community off Sendai Bay, northern Japan, was studied based on a total of 256 trawl samples collected during May, October and November, from 1989 to 1992. The samples were classified into 16 assemblages according to year, month and depth. The importance of E. pacifica to the total diet of each of the assemblages was measured using an index considering both fish diet and species composition. A total of 24 fish species out of 87 were found to ingest E. pacifica. Of these, Gadus macrocephaus and Theragra chalcogramma had the highest predation impact due to their dominance in the fish assemblages and the high proportion of E. pacifica in their diets. The contribution of E. pacifica to the total diet of the fish assemblage was highest in the shallow (≤300 m) regions during May, accounting for an average of 38.5% for the 4 years. However, during May 1990, when the warm Kuroshio Extension prevailed, the contribution was higher in the deep (〉300 m; 22.6%) region, reflecting active and/or passive movements of E. pacifica. The average predation impact was maximal in the May/shallow assemblage (mean ± SE; 4.6 ± 1.4 kg wet wt km2 d−1) and was minimal in the November/shallow assemblage (0.4 ± 0.3). Annual estimates of consumption by demersal fish fishes ranged from 43 to 128 metric tons, representing 15 to 64% of the annual commercial catch of E. pacifica by local fisheries. It is therefore suggested that the fisheries on E. pacifica have the potential to considerably impact the demersal fish assemblages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002270050386
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  • 9
    Electronic Resource
    Electronic Resource
    Interpretation of a peculiar behaviour of the critical current density with relative sintered density in small-grained YBa2Cu3Ox ceramics (1995)
    Springer
    Journal of materials science 14 (1995), S. 1220-1222 
    Details
    ISSN: 1573-4811
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00291812
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  • 10
    Electronic Resource
    Electronic Resource
    Down regulation of cAMP production by cAMP receptor protein in Escherichia coli: an assessment of the contributions of transcriptional and posttranscriptional control of adenylate cyclase (1996)
    Springer
    Molecular genetics and genomics 253 (1996), S. 198-204 
    Details
    ISSN: 1617-4623
    Keywords: Key words Adenylate cyclase ; cAMP synthesis ; CRP ; Transcriptional regulation ; Posttranscriptional regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Escherichia coli cells that are deficient in the cAMP receptor protein (CRP) overproduce cAMP. We and others have previously found that transcription of the adenylate cyclase gene (cya) is negatively regulated by the CRP-cAMP complex. Here, we have investigated the contribution of this transcriptional regulation to the control of cAMP levels. Several variants of the cya gene have been constructed and characterized with respect to their expression and their ability to produce cAMP. Overproduction of cAMP in a crp - background was reduced from 200-fold to 50-fold when transcriptional regulation by CRP-cAMP was eliminated by replacing the cya promoter with the constitutive bla promoter. When the C-terminal 48 amino acids of adenylate cyclase were deleted without changing the promoter, the degree of overproduction of cAMP was reduced to 4-fold. Finally, the increase in cAMP level observed in crp - cells was almost completely abolished when the truncated cyclase was expressed from the bla promoter. We conclude that transcriptional regulation of cya does indeed play a role in the down-regulation of cAMP production by CRP, although the major regulation is exerted at the posttranscriptional level. The C-terminal region comprising the last 48 amino acids of cyclase is responsible for the posttranscriptional regulation. A simple new method for the determination of cAMP is also described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050313
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