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  • 1
    Electronic Resource
    Electronic Resource
    Transport Mutants and Transport Genes of Corynebacterium glutamicum (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 782 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb40560.x
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  • 2
    Electronic Resource
    Electronic Resource
    Construction of l-Lysine-, l-Threonine-, or l-Isoleucine-Overproducing Strains of Corynebacterium glutamicum (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 782 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb40544.x
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Lumbar microdiscectomy. Technique and complications (1999)
    Springer
    Der Orthopäde 28 (1999), S. 593-597 
    Details
    ISSN: 1433-0431
    Keywords: Key words Lumbar disc • Discectomy • Complications • Microscope • Discherniation ; Schlüsselwörter Bandscheibe • Diskotomie • Komplikationen • Operationsmikroskop • Bandscheibenvorfall
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die technischen Besonderheiten der mikrochirurgischen lumbalen Bandscheibenoperation bestehen darin, daß neben einem speziellen Instrumentarium Lichtquellen erforderlich sind, die den schmalen Situs in der Tiefe genügend ausleuchten. Die besten Voraussetzungen bietet das Operationsmikroskop. Aber auch Kaltlichtquelle oder Stirnlampe stellen eine Alternative dar. Das Risiko der spezifischen Komplikation des mikrochirurgischen Zugangs – das Aufsuchen der falschen Etage – ist bei exakter präoperativer Planung minimierbar. Die anderen Komplikationen wie Duraverletzungen und Blutungen sind beim Vorgehen mit dem Operationsmikroskop wegen der besseren Sicht eher selten zu erwarten. Die schwerwiegenden Komplikationen, auch bei mikrochirurgischem Vorgehen, die Verletzung der ventralen großen Bauchgefäße läßt sich mit der neuentwickelten Faßzange vermeiden.
    Notes: Summary Lumbar Microdiscectomy requires special instruments and provides a good view with a narrow approach. The microscope is the best surgical aid. Alternatives are a retractorintigrated light or headlamp. A specific complication of lumbar microdiscectomy – the wrong level – can be minimized by an exact preoperative planning. Other complications like dural lesions and exessive bleeding are less frequent with the microscope because of the better view. The most severe complications that can occur with any lumbar disc operation, major vessel or visceral injury, can be avoided by lusing the new rongeur with a depth guard.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00003647
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  • 4
    Electronic Resource
    Electronic Resource
    Adaptation of the filamentous fungus Ashbya gossypii to hyperosmotic stress: different osmoresponse to NaCl and mannitol stress (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 219-226 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Osmoadaption mechanisms of the biotechnologically important hemiascomycete Ashbya gossypii were investigated, thereby distinguishing between halo- and osmotolerance by exposure to NaCl and mannitol stress. We studied the growth and ultrastructure of differently treated cells and quantified the intracellular contents of compatible solutes and inorganic ions. Mannitol affected growth of A. gossypii at concentrations above 0.8 M, whereas NaCl inhibited growth at 0.2 M. NaCl-treated cells differed from control cells in having smaller vacuoles, which occupied a smaller part of the cell volume. Glycerol was found to be the predominant compatible solute in A. gossypii; accumulation of inorganic ions could not be detected. Measurement of glycerol uptake under isosmotic conditions as well as upon hyperosmotic stress revealed the existence of a highly active glycerol-uptake system, which, however, was down-regulated under hyperosmotic stress. Investigation of glycerol biosynthesis by measuring glycerol-3-phosphate dehydrogenase activity under hyperosmotic conditions indicated that accumulation of glycerol in A. gossypii is almost solely due to biosynthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051280
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  • 5
    Electronic Resource
    Electronic Resource
    DNA hydrolysis by inorganic catalysts (1999)
    Springer
    Applied microbiology and biotechnology 52 (1999), S. 761-767 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Non-enzymatic reagents that efficiently promote the hydrolytic cleavage of DNA currently receive much attention since they have many potential applications in molecular biology. This review focuses on recent progress in the hydrolysis of the phosphodiester backbone of DNA by metal ions and metal complexes. Pioneering work on the sequence-selective DNA scission by an artificial restriction enzyme, which is prepared by covalent attachment of a cerium(IV) complex to an antisense-deoxyoligonucleotide, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051588
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  • 6
    Electronic Resource
    Electronic Resource
    Assessment of lung function in infants and young children with lung disease (1995)
    Springer
    European journal of pediatrics 154 (1995), S. S13 
    Details
    ISSN: 1432-1076
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02191497
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  • 7
    Electronic Resource
    Electronic Resource
    The age at onset of chronicPseudomonas aeruginosa colonization in cystic fibrosis —prognostic significance (1995)
    Springer
    European journal of pediatrics 154 (1995), S. S69 
    Details
    ISSN: 1432-1076
    Keywords: Cystic fibrosis ; Pseudomonas aeruginosa ; Lung involvement ; Survival ; Prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To evaluate the prognostic significance of the age at onset of chronicPseudomonas aeruginosa colonization (OPCP) with respect to pulmonary disease progression in patients with cystic fibrosis (CF), a retrospective long-term analysis using annual chest radiographs was performed on 54 CF patients. Thirtyseven patients (68%) were chronically colonized before the age of 12 years (group 1), 17 patients (32%) thereafter (group 2). These two groups did not significantly differ in terms of mean duration of follow up (16.2±5.9 years), sex, CF genotypes, colonization with other respiratory pathogens, supportive medical treatment and death rate during the study period. Chest radiographs were evaluated according to the Chrispin-Norman score, increasing scores representing increasing severity of respiratory disease. In both groups, progression of score means was not accelerated up to 6 years after OCPC (Scores at OCPC set 0; mean score ±SEM 6 years prior to OCPC −5.6±2.0; 10 years after OCPC +3.6±0.7 points). Patients chronically colonized prior to age 12 years (group 1) scored significantly higher between age 2 and 11 years (maximum difference at age 8 years [mean ±SEM]: 9.4±0.7 vs. 4.3±1.3 points;P= 0.002) as compared to group 2. After age 11 years, mean scores were similar in both groups, since in group 2 scores increased rapidly after age 8 years. We conclude that OCPC did not cause an immediate acceleration of CF lung disease judged by serial chest radiographs. Rapid progression in group 2 (OCPC after age 12 years) was independent of OCPC since it occurred earlier. These data indicate that OCPC may be a marker rather than the cause of respiratory disease progression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02191510
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  • 8
    Electronic Resource
    Electronic Resource
    Glutamine uptake by a sodium-dependent secondary transport system in Corynebacterium glutamicum (1995)
    Springer
    Archives of microbiology 164 (1995), S. 98-103 
    Details
    ISSN: 1432-072X
    Keywords: Key words Glutamine ; Amino acid transport ; Solute ; uptake ; Corynebacterium glutamicum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Corynebacterium glutamicum took up glutamine by a sodium-dependent secondary transport system. Both the membrane potential and the sodium gradient were driving forces. Glutamine uptake showed Michaelis-Menten kinetics, with a K m of 36 μM and a V max of 12.5 nmol min–1 (mg dry weight)–1 at pH 7. Despite a pH optimum in the alkaline range around pH 9, it was shown that uncharged glutamine is the transported species. The affinity for the cotransported sodium was relatively low; an apparent K m of 1.4 mM was determined. Among various substrates tested, only asparagine, when added in 50-fold excess, led to an inhibition of glutamine transport. It was concluded that glutamine uptake occurs via a specific transport system in symport with at least one sodium ion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050240
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  • 9
    Electronic Resource
    Electronic Resource
    Threonine diffusion and threonine transport in Corynebacterium glutamicum and their role in threonine production (1996)
    Springer
    Archives of microbiology 165 (1996), S. 48-54 
    Details
    ISSN: 1432-072X
    Keywords: Key words Amino acid transport ; Uptake ; Excretion ; Diffusion ; Threonine ; Corynebacterium glutamicum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transmembrane threonine fluxes (i.e., uptake, diffusion, and carrier-mediated excretion) all contribut-ing to threonine production by a recombinant strain of Corynebacterium glutamicum, were analyzed and quantitated. A threonine-uptake carrier that transports threonine in symport with sodium ions was identified. Under production conditions (i.e., when internal threonine is high), this uptake system catalyzed predominantly threonine/threonine exchange. Threonine export via the uptake system was excluded. Threonine efflux from the cells was shown to comprise both carrier-mediated excretion and passive diffusion. The latter process was analyzed after inhibition of all carrier-mediated fluxes. Threonine diffusion was found to proceed with a first-order rate constant of 0.003 min–1 or 0.004 μl min–1 (mg dry wt.)–1, which corresponds to a permeability of 8 × 10–10 cm s–1. According to this permeability, less than 10% of the efflux observed under optimal conditions takes place via diffusion, and more than 90% must result from the activity of the excretion carrier. In addition, the excretion carrier was identified by (1) inhibition of its activity by amino acid modifying reagents and (2) its dependence on metabolic energy in the form of the membrane potential. Activity of the excretion system depended on the membrane potential, but not on the presence of sodium ions. Threonine export in antiport against protons is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050295
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  • 10
    Electronic Resource
    Electronic Resource
    Isolation of the putP gene of Corynebacterium glutamicum and characterization of a low-affinity uptake system for compatible solutes (1997)
    Springer
    Archives of microbiology 168 (1997), S. 143-151 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsCorynebacterium glutamicum ; PutP ; Proline transport ; Compatible solutes ; Osmoregulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Corynebacterium glutamicum accumulates the compatible solutes proline, glycine betaine, and ectoine under conditions of high osmolality. Uptake of proline is mediated by both a high-affinity and a low-affinity secondary transport system. The low-affinity uptake system also accepts glycine betaine and ectoine as substrates. In the present study, the gene encoding the high-affinity proline uptake system PutP was isolated by heterologous complementation of Escherichia coli mutant strain WG389, which lacks the transport systems BetT, PutP, ProP, and ProU and is unable to synthesize proline and glycine betaine. This gene (putP) encodes a protein of 524 amino acids that shares identity with the proline transport systems PutP of E. coli, Staphylococcus aureus, Salmonella typhimurium, Haemophilus influenzae, and Klebsiella pneumoniae. Functional studies of PutP synthesized in E. coli mutant strain MKH13, which also lacks the transport systems for compatible solutes and is unable to synthesize glycine betaine, revealed that this carrier system is not regulated by the external osmolality on the level of activity. K m values of 7.6 mM for proline and 1.3 mM for sodium as cotransported ion were determined. Deletion of the putP gene allowed the functional characterization of another proline uptake system with low affinity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050480
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