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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 18 (1999), S. 919-923 
    ISSN: 1432-203X
    Keywords: Key words Callus ; Explant source ; Multiple shoot-bud induction ; Gelrite ; Shoot cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Adventitious shoot buds were induced from leaf and stem explants of Bacopa monnieri on Murashige and Skoog medium supplemented with benzyladenine or kinetin. The source of the explants as well as different gelling agents in the medium were found to influence shoot induction and eventual shoot growth. The best response was obtained in leaf explants taken from shoot cultures grown in medium supplemented with 2 μM benzyladenine and gelled with 0.2% gelrite. A transverse section of the leaf explant incubated in this medium showed several shoot primordia emerging from the leaf surface. This system exhibited a potential for repeated harvesting of the shoots from the original leaf explant as the latter continued to expand and regenerate new shoots, upon repeated periodical subculturing onto fresh medium. However, the callusing response of the plant was very low. Qualitative TLC studies of the regenerated shoots revealed a phytochemical profile similar to that of the field grown-plants.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1084
    Keywords: Key words: Aortic arch syndrome ; Arteries ; Renal transluminal angioplasty ; Arteritis ; Hypertension ; Aortitis ; Takayasu's arteritis ; Fibromuscular dysplasia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. The aim of this study was to evaluate the feasibility of coaxial approach in difficult-to-cross lesions in patients with failed percutaneous transluminal renal angioplasty by conventional over-the-wire exchange technique. Twelve stenoses in 10 patients (six women and four men; age range 19 ± 7 years) with uncontrolled hypertension were treated by this method. The stenosis was caused by nonspecific aortoarteritis in 8 patients and fibromuscular dysplasia in 2 patients. It was ostial in seven and post-ostial in five vessels. Conventional exchange technique was unsuccessful in all of them. All procedures were done by femoral route. Technical success was seen in 11 (92 %), without complication. The stenosis improved from 90 ± 2.1 % (range 80–100 %) to 6 ± 7 % (range 0–20 %), blood pressure decreased from 198 ± 12.3 mm Hg (range 180–220 mm Hg)/130 ± 6.7 mm Hg (range 120–140 mm Hg) to 119 ± 5.7 mm Hg (range 110–130 mm Hg)/83 ± 3.9 mm Hg (range 80–90 mm Hg), and number of drug treatments for hypertension fell from 3.6 ± 0.52 (range 3–4) to 1 ± 0.94 (range 0–3; p 〈 0.01). Percutaneous transluminal renal angioplasty resulted in “cure” in 3 patients and “improvement” in 7 patients. Follow-up period was 3–21 months (mean 6.4 months). No restenosis was detected. Coaxial approach is safe and effective in treating difficult-to-cross lesions in which renal angioplasty by conventional exchange technique is unsuccessful.
    Type of Medium: Electronic Resource
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