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  • 1
    Electronic Resource
    Electronic Resource
    Optical resonator matching for JAERI free electron laser (1998)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 69 (1998), S. 327-328 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: An optical resonator length of free electron lasers must be exactly matched with the frequency of the repeated electron beam from the accelerator driver so the incident electron beam can overlap with stored light pulses in the resonator. We made the exact resonator length matching by injecting ultrashort laser light pulses in the resonator as external clock signals. The external laser was a mode-locked Ti:Sapphire laser with 80 fs pulse width and synchronized with the accelerator clock signals. With the simultaneous use of a streak camera and a photo diode, we succeeded in making the quick matching within an accuracy of 1 μm or less in a direct and exact manner. © 1998 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1148523
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  • 2
    Electronic Resource
    Electronic Resource
    Separable Regulatory Elements from Pro-α2(XI) Collagen Gene Drive Distinct Patterns of Cartilage-Specific Expression in Transgenic Mice (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 785 (1996), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1996.tb56303.x
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  • 3
    Electronic Resource
    Electronic Resource
    Apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pig ovaries (1996)
    Springer
    Cellular and molecular life sciences 52 (1996), S. 647-651 
    Details
    ISSN: 1420-9071
    Keywords: Apoptosis ; Ca2+/Mg2+-dependent endonuclease ; cumulus cell ; granulosa cell ; pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The porcine antral follicles, 3–6 mm in diameter, were dissected from the ovaries of mature pigs, and then granulosa and cumulus cells were isolated from each follicle. In atretic follicles, high activity of neutral Ca2+/Mg2+-dependent endonuclease and DNA ladder formation, estimated by electrophoresis, were noted in granulosa cells but not in cumulus cells. Extremely low activity of the endonuclease and no DNA ladder formation were observed in both types of cells obtained from healthy follicles. Moreover, apoptotic cells were observed histochemically among granulosa cells only. A good correlation (r=0.987) between the endonuclease activity of granulosa cells and the progesterone/estradiol ratio of follicular fluid in each follicle was found. These results suggest that apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pigs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01925566
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  • 4
    Electronic Resource
    Electronic Resource
    Nephritogenicity and α-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane (1996)
    Springer
    Virchows Archiv 428 (1996), S. 281-288 
    Details
    ISSN: 1432-2307
    Keywords: Glomerulonephritis ; Nephritogenic antigen ; Glomerular basement membrane ; Type IV collagen ; NC1 ; Monoclonal antibody ; Goodpasture's syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Nephritogenicity (anti-GBM-nephritis-inducing activity) and α-chain composition of globular-domain (NC1) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different α chains of type IV collagen. A purified nephritogenic fraction from renal BM contained α1–α6(IV)NC1, whereas a non-nephritogenic fraction contained only α1–α2(IV)NC1. Renal and pulmonary NC1 had strong nephritogenic activity; placental NC1 had weak activity. The renal and pulmonary fractions contained α1–α6(IV)NC1, and the placental fraction had a large amount of α1–α2(IV)NC1 and a very small amount of α3–α6(IV)NC1. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained α1–α5(IV) chains, but not the α6(IV) chain. The absence of α6(IV) chain in glomerular BM in bovine and in humans indicates that α6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of α3–α5(IV)NC1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196702
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  • 5
    Electronic Resource
    Electronic Resource
    Immunological diagnosis of Werner syndrome by down-regulated and truncated gene products (1999)
    Springer
    Human genetics 〈Berlin〉 105 (1999), S. 301-307 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Although immunological methods are widely used to diagnose various infectious diseases, they have rarely been employed to detect genetic diseases. In this study, we have established an immunoblot analysis system for the diagnosis of Werner syndrome (WS), a recessive genetic disorder causing premature aging and an enhanced risk of rare cancers. The method uses an immunoblot technique with specific monoclonal antibodies to WS gene product, and B-lymphoblastoid cell lines (LCLs) transformed by Epstein-Barr virus; these cell lines express an increased level of normal WS gene product DNA helicase. The method clearly distinguishes normal from patient LCLs containing any of the mutation types found so far in Japan, primarily because of the drastically reduced levels of mutated gene products, and secondarily because of the truncated product sizes. A comparison of this immunological diagnosis with the symptom-based clinical diagnosis has narrowed down the criteria of symptoms essential for WS diagnosis. This procedure is compatible with, and has some advantage over, the genetic method, because WS patients can be diagnosed without determining the mutated gene sequences. The method exemplified in WS may also be applied to detect some other genetic diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004399900151
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