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  • 1
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: The rat substance P (SP) receptor (SPR) was expressed in insect Sf9 cells by infection with recombinant baculovirus. The receptor bound SP with high affinity (KD = 360 pM) and had a rank order of affinity of SP 〉 neurokinin A 〉 neurokinin B. Ligand activation of the receptor resulted in an increase in both inositol lipid hydrolysis and intracellular Ca2+ concentration ([Ca2+]i). However, high-level expression of the receptor, in the absence of ligand, was correlated with increased basal turnover of inositol lipids and an elevated rate of Ca2+ influx. These results demonstrate that the Sf9 cells provide a suitable environment for the high-level expression of a functionally active SPR. Two carboxy-terminal epitope-tagged receptors (SPR-KT3 = SPR-TPPPEPET, COOH; SPR-Glu = SPR-EEEEYMPME, COOH) were also expressed. The affinity of the KT3-tagged receptor for ligand was similar to that of the wild-type receptor (KD = 405 pM), and that of the Glu-tagged receptor was slightly lower (KD = 1,082 pM). The high-affinity SP binding site of all three receptors was sensitive to guanosine 5′-O-(3-thiotriphosphate) pretreatment. The maximal signal-transducing ability of the epitope-tagged receptors was comparable to that of the wild-type receptor ([Ca2+]i rise as a percentage of wild-type: SPR-KT3, 80–100%; SPR-Glu, 88–100%). These data show that heterologous expression in the baculovirus system results in high expression of functional wild-type and tagged receptors.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 79 (1996), S. 7084-7089 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: A first-principles calculation of the magnetic anisotropy energies of X/Co (X=Pt, Cu, Ag and Au) metallic multilayers has been performed to investigate the effect of strain on the magnetic anisotropy. It is successfully predicted that Pt/Co and Au/Co multilayers exhibit perpendicular magnetic anisotropies in accordance with experiments. The strength of the hybridization of electronic states at the interface determines the relative position of the Fermi energy to the local density of states (LDOS) of ||m||=2 character of Co d electrons of minority spin. If the LDOS of ||m||=2 character is large at the Fermi energy, a system shows a perpendicular anisotropy. The anisotropy energies of all the systems increase as a function of interatomic distance in the in-plane direction. The magneto-elastic constants are more than twice the value of pure Co. The origin of this large value is explained in terms of the change of relative position of the Fermi level to that of the LDOS of ||m||=2 character as the value of strain increases. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 171-174 (Oct. 1999), p. 717-724 
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    International journal of thermophysics 17 (1996), S. 441-454 
    ISSN: 1572-9567
    Keywords: aqueous solutions ; compressibility ; density ; dioxane ; high pressure ; partial molar volume
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Densities of aqueous of 1,4-dioxane have been measured at temperatures from 298 to 348 K and at pressures up to 40 MPa by a vibrating-tube method. Molar volumes obtained with an estimated uncertainty of ±0.2°,% are correlated with pressure by the Tait equation within the experimental uncertainty. Pressure and composition dependences of the excess molar volume, partial molar volume, and isothermal compressibility are determined and they are compared with those of other aqueous solutions.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Colloid & polymer science 106 (1997), S. 150-157 
    ISSN: 1435-1536
    Keywords: Polymer solutions ; shear flow ; phase separation ; viscoelasticity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract We numerically investigate nonlinear regimes of shear-induced phase separation in entangled polymer solutions. For the purpose a time-dependent Ginzburg-Landau model describing the two-fluid dynamics of polymer and solvent is used. A conformation tensor is introduced as a new dynamic variable to represent chain deformations. Its variations give rise to a large viscoelastic stress. Above the coexistence curve, a dynamical steady state is attained, where fluctuations are enhanced on various spatial scales. At relatively large shear, the elongated polymer-rich regions form a transient network supporting most of the stress. Because such a network is continuously deformed in shear flow, the shear stress and the normal stress difference exhibit large fluctuations. This result explains an early observation of large temporal fluctuations of the normal stress by Lodge.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of materials science 33 (1998), S. 2047-2052 
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Mechanical properties of fine-grained TiN were studied by compression tests at temperatures ranging from 1073–1823 K and strain rates from 2×10-5 to 5×10-3 s-1. The temperature dependence of maximum (fracture or peak) stress of TiN reveals three regions with different activation energy and strain-rate sensitivity. At lower temperatures, brittle fracture takes place without plastic deformation. Fracture stress is independent of temperature, and greater than 1 Gpa. In the intermediate temperature region, specimens fracture in a quasi-brittle manner after a few per cent plastic deformation. Fracture stress decreases above 1300 K (in the intermediate temperature region), due to the deformation-assisted fracture. TiN becomes fully ductile at further higher temperatures, at which five independent slip systems are available. This ductile to brittle transition characteristic of TiN is similar to MgO (ionic) but different from TiC (covalent), though all three materials take the same NaCl type of lattice structure. In the high-temperature region, the activation energy for plastic deformation is close to that for diffusion of nitrogen in TiN. Strain-rate sensitivity in this region is typical of superplasticity, suggesting the possibility of superplastic forming. © 1998 Chapman and Hall
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 43 (1998), S. 89-98 
    ISSN: 0021-9304
    Keywords: gelatin ; films ; tissue ; bonding ; argon beam ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Cross-linked gelatin films were bonded to heart muscle and to lung pleura and parenchyma using the electrical discharge of an argon beam radiofrequency coagulator. The bonds were stable in warm saline buffer for minutes to hours. Bonding was thought to partly occur through a mechanical interlock of film and tissue elements. The interdigitation of tissue and film arose during exposure to the argon beam, which denatured protein constituents of both, and created a fluidized state that rapidly coalesced. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res (Appl Biomater) 43: 89-98, 1998
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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