ISSN:
1432-0584
Keywords:
Bromelin
;
Antibody screening
;
α2-Macroglobulin
;
Thiol proteinase inhibitor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Human sera contain bromelin inhibitors in irregular antibody screening techniques. In order to identify and purify these inhibitors, pooled human sera were precipitated with ammonium sulfate, followed by DEAE-cellulose and Sephadex G-150 column chromatography. Proteinase activity and inhibitory activity to bromelin were observed in two protein fractions, these were identified as α2-macroglobulin and the S-2 thiol proteinase inhibitor.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00326028
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