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The identification of inhibitors in bromelin one-stage irregular antibody screening techniques (1987)

Ogasawara, K. ; Mazda, T. ; Shimizu, M.

Springer

Annals of hematology 54 (1987), S. 51-55

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ISSN: 1432-0584

Keywords: Bromelin ; Antibody screening ; α2-Macroglobulin ; Thiol proteinase inhibitor

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Human sera contain bromelin inhibitors in irregular antibody screening techniques. In order to identify and purify these inhibitors, pooled human sera were precipitated with ammonium sulfate, followed by DEAE-cellulose and Sephadex G-150 column chromatography. Proteinase activity and inhibitory activity to bromelin were observed in two protein fractions, these were identified as α2-macroglobulin and the S-2 thiol proteinase inhibitor.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00326028

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