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  • 11
    Electronic Resource
    Electronic Resource
    Characterization of fungal melanins and soil humic acids by chemical analysis and infrared spectroscopy (1990)
    Springer
    Biology and fertility of soils 10 (1990), S. 72-76 
    Details
    ISSN: 1432-0789
    Keywords: Brazilian soils ; Fungal melanins ; Soil humic acid ; Infrared analysis ; Latosols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Humic acids from two Brazilian topsoils under savanna grassland and five soil fungal melanins were characterized by elemental, functional group and infrared analysis. C, N, total acidity, COOH, and phenolic OH contents were within the ranges reported for several other fungal melanins and soil humic acids. Compared with the soil humic acids, the infrared spectra of the fungal melanins showed greater detail, indicative of higher aliphaticity. They were similar to the type III infrared spectra of humic acids, which are characteristically high in proteinaceous material and polysaccharides. The infrared spectra of the humic acids from the two Brazilian soils studied were classified as type I, which includes most soil humic acids. Notwithstanding the greater detail, in some areas the fungal melanin spectra were similar to those reported for other fungal melanins and humic acids of different origins. The probable contribution of the melanic fungi to the formation of soil humic polymers is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336128
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  • 12
    Electronic Resource
    Electronic Resource
    Light microscopical detection of inter-alpha-trypsin inhibitor and its different mRNAs in cultured hepatoma Hep G2 cells using immunocytochemical and in situ hybridization techniques (1994)
    Springer
    Journal of molecular histology 26 (1994), S. 252-261 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Hep G2 hepatoma cell line synthesizes the inter-alpha-trypsin inhibitor (ITI). This protease inhibitor and the other proteins of this family include four polypeptides chains: three heavy chains (HC1, HC2, HC3) and one light chain (bikunin). In the present study, we have demonstrated by immunofluorescence that ITI is detected mainly in perinuclear cytoplasmic zones comparable to those of albumin or alpha-1-antitrypsin. The presence of the mRNAs of the four polypeptide chains in all Hep G2 cells of a non-synchronized culture have been demonstrated by in situ hybridization. An evaluation of the transcription of the four ITI genes through an analysis of markings brings to the fore a clearly much higher rate of mRNAs from the light chain than from the heavy chains. The mRNAs corresponding to the HC2 chains are more heavily represented than are those corresponding to the HC1 and HC3 chains. In Hep G2 cells in culture, a quantification of mRNAs based on the in situ hybridization technique shows that their relative quantities, in decreasing order, are those of L, HC2, HC3 and HC1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166106
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  • 13
    Electronic Resource
    Electronic Resource
    Light microscopical detection of inter-alpha-trypsin inhibitor and its different mRNAs in cultured hepatoma Hep G2 cells using immunocytochemical andin situ hybridization techniques (1994)
    Springer
    Journal of molecular histology 26 (1994), S. 252-261 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Hep G2 hepatoma cell line synthesizes the inter-alpha-trypsin inhibitor (ITI). This protease inhibitor and the other proteins of this family include four polypeptides chains: three heavy chains (HC1, HC2, HC3) and one light chain (bikunin). In the present study, we have demonstrated by immunofluorescence that ITI is detected mainly in perinuclear cytoplasmic zones comparable to those of albumin or alpha-1-antitrypsin. The presence of the mRNAs of the four polypeptide chains in all Hep G2 cells of a non-synchronized culture have been demonstrated byin situ hybridization. An evaluation of the transcription of the four ITI genes through an analysis of markings brings to the fore a clearly much higher rate of mRNAs from the light chain than from the heavy chains. The mRNAs corresponding to the HC2 chains are more heavily represented than are those corresponding to the HC1 and HC3 chains. In Hep G2 cells in culture, a quantification of mRNAs based on thein situ hybridization technique shows that their relative quantities, in decreasing order, are those of L, HC2, HC3 and HC1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02388440
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  • 14
    Electronic Resource
    Electronic Resource
    Respiratory symptoms and pulmonary function in 871 miners according to Pi phenotype: A longitudinal study (1988)
    Springer
    European journal of epidemiology 4 (1988), S. 39-44 
    Details
    ISSN: 1573-7284
    Keywords: Epidemiology ; Occupational pathology ; Chronic bronchitis Iron miners ; Protease inhibitor (Pi) phenotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pi phenotype was determined and α1 protease inhibitor (α1Pi) was measured in 871 iron-ore miners examined twice at five years interval. A questionnaire on respiratory symptoms was administered and lung function tests: spirometry (VC, FEV1.0), measurements of the residual volume (RV/TLC) and of the CO diffusion (FuCO), were carried out by the same medical and technical team, using the same apparatus. There were no differences in age, lenght of employment or smoking habits among the three Pi Phenotype groups: M (90:03%), MS (7.1%), MZ (2.8%). The prevalence of clinical symptoms at the initial survey and the incidence of symptoms between the 2 surveys were not related to Pi Phenotype groups. However a significantly steeper decline of FEV1.0/VC was observed in the α1Pi partially deficient groups MS and MZ (−3.9%) compared with the non-deficient group (−1.8%). No significant difference was observed for VC, RV /TLC and FuCO. This finding suggests that an intermediate deficiency of α1Pi may be related to a slight but statistically significant impairment of a lung function in occupational conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00152690
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  • 15
    Electronic Resource
    Electronic Resource
    The band structure in124Ba (1987)
    Springer
    The European physical journal 326 (1987), S. 337-338 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excited states of124Ba have been studied using the96Zr (34S,6n) reaction at 160 MeV. The yeast band is observed up to a 1π = 22+ state with a band crossing at ℏw = 0.37 MeV. Negative parity levels ate also identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01297590
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  • 16
    Electronic Resource
    Electronic Resource
    Galileo Probe Nephelometer experiment (1992)
    Springer
    Space science reviews 60 (1992), S. 179-201 
    Details
    ISSN: 1572-9672
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The objective of the Nephelometer Experient aboard the Probe of the Galileo mission is to explore the vertical structure and microphysical properties of the clouds and hazes in the atmosphere of Jupiter along the descent trajectory of the Probe (nominally from 0.1 to 〉 10 bars). The measurements, to be obtained at least every kilometer of the Probe descent, will provide the bases for inferences of mean particle sizes, particle number densities (and hence, opacities, mass densities, and columnar mass loading) and, for non-highly absorbing particles, for distinguishing between solid and liquid particles. These quantities, especially the location of the cloud bases, together with other quantities derived from this and other experiments aboard the Probe, will not only yield strong evidence for the composition of the particles, but, using thermochemical models, for species abundances as well. The measurements in the upper troposphere will provide ‘ground truth’ data for correlation with remote sensing instruments aboard the Galileo Orbiter vehicle. The instrument is carefully designed and calibrated to measure the light scattering properties of the particulate clouds and hazes at scattering angles of 5.8°, 16°, 40°, 70°, and 178°. The measurement sensitivity and accuracy is such that useful estimates of mean particle radii in the range from about 0.2 to 20 μ can be inferred. The instrument will detect the presence of typical cloud particles with radii of about 1.0 μ, or larger, at concentrations of less than 1 cm3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216854
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