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  • 1990-1994  (2)
  • 1980-1984  (3)
  • 1975-1979  (3)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 36 (1992), S. 0 
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Molecules encoded by the class I major histocompatibility genes bind short (nonameric) peptides produced by inlracellular proleolysis of antigens. These complexes formed intraeellularly are then expressed on membranes of target eells and recognized by the anligen receptor of eytolylic T cells. No binding of externally added peptides could so far be monitored directly on the antigen presenting cells, although cytotoxicity experiments and indirect binding assays provided evidence for its existence. Here we report experiments where specific binding to class I molecules, of externally added peptides, has been monitored on living cells, N-terminal biottn-labelled Kd-restricted peptides (residues 147-155, residues 147-158, and an analogue lacking the arginine at position 156. derived from the sequence of the influenza A virus nucleoprotein) were incubated with murine H-2Kd mastocytoma cells (line P815)dt 4°C. The binding on surface of hve, intact cells was then demonstrated fluoro metrically via ihe inieraction ofa streptavidin-phycoerythrin conjugate with the biotin-labelled peptides. Thus, this binding does not involve processing, and its specificity in terms of peptide structure was established by competition with the respective unmodified peptides. The specificity ofbinding to class I molecules was demonstrated by blocking experiments u.sing monoclonal antibodies specific for H-2Kd. Finally, a correlation was observed between the results of peptide binding measurements and those ofcytotoxieity assays.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 306 (1978), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Inflammation research 36 (1992), S. C187 
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We had found that histamine (HA)-induced relaxation of cavian pulmonary artery is selectively inhibited by Nω-methyl-l-arginine (NMA) and thus depends on the synthesis ofl-arginine (ARG)-derived EDRF/nitric oxide (NO). We have now assessed whether ARG-derived NO also mediates the coronary-vasodilating effect of HA in the intact heart. Langendorff guinea pig hearts, vasoconstricted by the thromboxane agonist U46619 (86 nM), responded to HA (bolus intra-aortic injections of 1–30 μg) with dose-dependent increases in coronary flow (20–90%), heart rate and contractility. NMA (92 μM) markedly inhibited the HA-induced increase in coronary flow, but not its positive inotropic and chronotropic effects. A similar inhibition was obtained with the NO scavenging compound, Fe2+-myoglobin. ARG (1 mM) reestablished the coronary-dilating effects of HA in the presence of NMA. Thus, ARG-derived NO contributes significantly to HA-induced coronary-vasodilatation. Inasmuch as the ultimate HA effect on the coronaries depends on the balance between constriction and relaxation, mediated by smooth muscle and endothelial H1-receptors, respectively, as well as relaxation due to smooth muscle H2-receptors, dysfunction of the NO system is likely to precipitate HA-induced vasospasm.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    European biophysics journal 10 (1983), S. 129-142 
    ISSN: 1432-1017
    Keywords: Photoreceptor noise ; Phototransduction ; Tetrodotoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Voltage noise analysis was performed on membrane potentials recorded from the photoreceptor cells of the honeybee drone. Voltage power spectra showed an increase in noise amplitude on passing from the dark to moderate light; with strong light noise fell to near the dark level. At intermediate light intensities the noise was reduced by application of tetrodotoxin. The probable effects on the power spectra of electrical coupling between cells are discussed. It is tentatively estimated that the unitary event in weak light is a conductance increase of 20 pS. The results are compared with previous results from photoreceptors of other species that appear to be specially adapted to weaker illuminations.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Functional analysis and its applications 9 (1975), S. 164-165 
    ISSN: 1573-8485
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Functional analysis and its applications 17 (1983), S. 229-230 
    ISSN: 1573-8485
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Experimental mechanics 15 (1975), S. 375-381 
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The configuration of three-dimensional strain rosettes is shown to be directly related to precision and reliability. Equations are given for computing these parameters, and several rosette configurations are compared accordingly. Experimental techniques developed at Istituto di Metrologia “G. Colonnetti” are described. A case history is given, in which the analysis of deviations between expected and actual embedded rosette results throws some light on complex interactions between model and deadweight loading machine.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Experimental mechanics 21 (1981), S. 341-348 
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Design data for a family of ring-shaped elastic elements are derived using analytical, numerical and experimental methods. The development of a family of elastic elements for load cells operating at medium to low load level is considered in detail, using several approaches. Snapes analyzed were circular ring with integral bosses and ‘square’ ring. The effects of thickness, boss size and fillet radius (reduced to nondimensional form) on strain/load and defiection/load relationships are evaluated within a given sample space, considering a two-dimensional problem. Thin-ring and curved-beam theory support the analytical approach, with correction terms covering some departures from theoretical shape. Numerical analysis with the finite-element method was mainly used to evaluate the effects of boss size and shape. Accordingly, the required grid patterns were developed using a mesh-generating method capable of covering the range of parameters considered. A set of configurations was selected, enabling single and combined effects to be analyzed with little entanglement between estimates. For the square ring, frame theory and a limit solution for the case of the plate with central hole are resorted to. Photoelastic tests were performed, covering a range of shapes by sequential remachining of the model, and equal contigurations were analyzed with the finite-element method. Strain-gage tests on actual elastic elements were made, considering also the results of a series of tests carried out at the Metrology Institute some time ago. Results obtained yield comprehensive information on the effect of several parameters on the load-output transfer function of ring-shaped elastic elements.
    Type of Medium: Electronic Resource
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