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  • 1
    Electronic Resource
    Electronic Resource
    Determination of Calcium Content in Individual Biological Cells by Inductively Coupled Plasma Atomic Emission Spectrometry (1994)
    s.l. : American Chemical Society
    Analytical chemistry 66 (1994), S. 3000-3004 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac00091a004
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  • 2
    Electronic Resource
    Electronic Resource
    Partial kidney transplantation: a successful kidney transplantation in a child with severe cardiac failure by surgical mass reduction of an adult donor kidney (1993)
    Springer
    Transplant international 6 (1993), S. 173-175 
    Details
    ISSN: 1432-2277
    Keywords: Kidney transplantation, child ; Child, kidney transplantation, reduced size ; Reduced size kidney transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report on a trial of partial kidney transplantation performed on a low body weight child with impaired cardiac function due to mitral valve stenosis and uremic cardiomyopathy. The weight of the donated kidney was successfully reduced by one-third using bench surgery in order to obtain sufficient graft perfusion and function. Our procedure is justified when a graft is too large to be adequately perfused in a recipient suffering from cardiac failure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00336364
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  • 3
    Electronic Resource
    Electronic Resource
    An electron microscopic study of glomerular lesions in hereditary nephrotic mice (ICGN strain) (1990)
    Springer
    Virchows Archiv 417 (1990), S. 223-228 
    Details
    ISSN: 1432-2307
    Keywords: Mouse ; Nephrotic syndrome ; Hereditary nephritis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glomerular lesions in hereditary nephrotic mice (ICGN strain) were investigated by electron microscopy. The glomeruli of unaffected animals, which appeared normal by light microscopy, had developed an ultrastructural change in the glomerular capillary basement membrane (GCBM). There was a partial thickening of the GCBM with bilaminar splitting of the lamina densa and an electron-dense fibrillar material exhibiting cross-striations. In affected animals, light microscopy revealed a marked thickening of GCBM and an increase of mesangial matrix without cellular proliferaton. By electron microscopy, multilaminar splitting of the lamina densa in the thickened GCBMs and fusion of the epithelial foot processes were observed. In some severely affected animals, immune complex deposition was found in GCBM, but little if any was observed in other animals. In the end, the glomeruli were globally sclerosed. Our findings suggest that initial structural abnormalities in GCBM may play an important role in the onset and development of the disease, though subsequent events such as immune complex deposition would modify the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01600137
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  • 4
    Electronic Resource
    Electronic Resource
    Separation and determination of dissolved and particulate humic substances in river water (1994)
    Springer
    Microchimica acta 113 (1994), S. 269-276 
    Details
    ISSN: 1436-5073
    Keywords: humic substances ; suspended particles ; membrane-filter dissolution ; surfactant-coated filter ; sorption ; spectrophotometry ; river water analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Distribution of humic and fulvic acids in participate or dissolved form is studied by using simple leaching and sorption techniques. After filtration of water sample (100–200 ml), the filter along with suspended particles is treated with 5 ml of chloroform and 3 ml of 0.1 mol/l sodium hydroxide solution. The filter dissolves completely in the organic phase, while the suspended particles remain in the aqueous phase enabling a leaching of humic substances. The leaching is repeated once more with 2 ml of 0.1 mol/l sodium hydroxide solution. The humic and fulvic acids in the combined solution are fractionated at pH l by filtration, where the membrane filter is preliminarily coated with sodium dodecyl sulfate. On the other hand, dissolved humic substances are concentrated from a 50-ml filtered sample by sorption on a DEAE-cellulose column. They are desorbed with 5 ml of 0.1 mol/l sodium hydroxide solution and fractionated at pH 1. The spectrophotometric analysis of river water reveals that fulvic acid is predominant in suspended particles as well as in filtered samples. The concentration of dissolved humic and fulvic acids is approximately ten times that of suspended particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01243617
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  • 5
    Electronic Resource
    Electronic Resource
    Collection of trace elements on tin(IV) hydroxide (1974)
    Springer
    Microchimica acta 62 (1974), S. 915-920 
    Details
    ISSN: 1436-5073
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Mikrogrammengen Fe, Co, Cu und Zn können in wäßrigen Lösungen an Zinn(IV)hydroxidniederschlägen bei pH 4–8 zu mehr als 95% angereichert werden. Sn kann als Bromid leicht abgedampft werden. Dieses Trennverfahren wurde zur emissionsspektrographischen Bestimmung geringer ppm-Verunreinigungen in hochgereinigtem Magnesiummetall angewendet.
    Notes: Summary Microgram quantities of iron, cobalt, copper, and zinc in aqueous solutions can be collected on tin(IV) hydroxide precipitates in greater than 95% yields over the range of pH 4 to 8. Tin is easily removed by evaporation as bromide. This separation technique has been applied to the emission spectrographic determination of low ppm level impurities in high-purity magnesium metal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01218313
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  • 6
    Electronic Resource
    Electronic Resource
    Coprecipitation with metal hydroxides for the determination of beryllium in seawater by graphite furnace atomic absorption spectrometry (1994)
    Springer
    Microchimica acta 117 (1994), S. 7-13 
    Details
    ISSN: 1436-5073
    Keywords: beryllium ; seawater ; Coprecipitation ; magnesium hydroxide ; tin(IV) hydroxide ; graphite furnace atomic absorption spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Coprecipitation first with magnesium hydroxide, next with tin(IV) hydroxide is developed for the determination of traces of beryllium in sea-water. To a 200-ml sample is added a sodium hydroxide solution to form magnesium hydroxide at pH 11.5, on which beryllium is quantitatively coprecipitated. The precipitate is separated by centrifugation and dissolved in 2 ml of 12 mol/l hydrochloric acid. The resulting solution (ca. 10 ml) is mixed with 2 mg of tin (IV) carrier and the pH is adjusted to 5.0 to collect the beryllium on tin (IV) hydroxide, leaving magnesium ions in the solution. The tin (IV) hydroxide is centrifuged, dissolved in 0.1 ml of 5 mol/l hydrobromic acid, and then diluted to 1 ml with water. Magnesium is so added as to be 500 μg/ml for increasing the sensitivity about four times, and the beryllium in the solution is determined by graphite furnace atomic absorption spectrometry. The experiments with synthetic seawater samples showed that pg — μg amounts of beryllium can be coprecipitated on the metal hydroxides and beryllium at the low ng/1 level can be determined with reasonable precision (RSD 〈 10%). The detection limit of the proposed method is 0.5 ng/l of beryllium in seawater.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01243011
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of impurities in bismuth by solid-liquid extraction and emission spectrography (1970)
    Springer
    Microchimica acta 58 (1970), S. 1095-1100 
    Details
    ISSN: 1436-5073
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Hochgereinigtes Wismutmetall oder Wismutnitrat werden in Salpetersäure gelöst und durch Erhitzen in festes basisches Wismutnitrat übergeführt. Verunreinigungen wie Eisen, Kobalt, Kupfer und Zink in ppm- oder ppb-Mengen werden aus der festen Probe mit Wasser extrahiert und emissionsspektrographisch bestimmt.
    Notes: Summary A high-purity bismuth metal or bismuth nitrate sample is dissolved in nitric acid and converted to solid basic bismuth nitrate by heating. Impurities such as iron, cobalt, copper, and zinc at the low ppm or ppb level are extracted from the solid with water and determined by DC arc emission spectrography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01215941
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  • 8
    Electronic Resource
    Electronic Resource
    C4 genes of the chimpanzee, gorilla, and orang-utan: evidence for extensive homogenization (1992)
    Springer
    Immunogenetics 35 (1992), S. 16-23 
    Details
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The human complement component 4 is encoded in two genes, C4A and C4B, residing between the class I and class II genes of the major histocompatibility complex. The C4A and C4B molecules differ in their biological activity, the former binding more efficiently to proteins than to carbohydrates while for the latter, the opposite holds true. To shed light on the origin of the C4 genes we isolated cosmid clones bearing the C4 genes of a chimpanzee, a gorilla, and an orang-utan. From the clones, we isolated the fragments coding for the C4d part of the gene (exons and introns) and sequenced them. Altogether we sequenced eight gene fragments: three chimpanzee (Patr-C4-1 *01, Patr-C4-1 *02, Patr-C4-2 *01), two gorilla (Gogo-C4-1 *01, Gogo-C4-2 *01), and three orang-utan (Popy-C4-1 *01, Popy-C4-2 *01, Popy-C4-3 *01). Comparison of the sequences with each other and with human C4 sequences revealed that in the region believed to be responsible for the functional difference between the C4A and C4B proteins the C4A genes of the different species fell into one group and the C4B genes fell into another. In the rest of the sequence, however, the C4A and C4B genes of each species resembled each other more than they did C4 genes of other species. These results are interpreted as suggesting extensive homogenization (concerted evolution) of the C4 genes in each species, most likely by repeated unequal, homologous, intragenic crossing-over.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216622
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  • 9
    Electronic Resource
    Electronic Resource
    Renal pathology in rats bearing tumour-secreting growth hormone (1991)
    Springer
    Pediatric nephrology 5 (1991), S. 533-538 
    Details
    ISSN: 1432-198X
    Keywords: Growth hormone ; Insulin-like growth factor-I ; Glomerular hypertrophy ; Glomerulosclerosis ; Glomerular hyperperfusion ; Rats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of growth hormone (GH) on renal structure and function were investigated in rats aged 10–16 weeks bearing a tumour secreting GH. Body weight gain, food intake, urine volume, and urinary excretion of cretinine and urea nitrogen were significantly greater in tumour-bearing rats than in controls. The tumour-bearing rats presented progressive proteinuria, hyperproteinaemia, and hyperlipidaemia. Creatinine clearance was significantly higher in experimental animals during the early experimental stage, but decreased as the glomerular lesions progressed, associated with a rise in serum creatinine levels. The glomeruli became progressively enlarged with degenerative changes of the visceral epithelial cells and capsular adhesions. In advanced stages proteinaceous material invaded the subcapsular space and the capillary lumen collapsed finally leading to glomerulosclerosis. Except for the presence of proteinaceous material and damaged epithelial cells the glomerular lesions resemble those observed experimentally after reduction of renal mass, and in diabetes mellitus. We speculate that the pathological features described are due to effects of persistently high levels of circulating GH on the glomerular cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01453696
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  • 10
    Electronic Resource
    Electronic Resource
    Somatic growth in corticosteroid-trated rats with passive Heymann nephritis —effects of recombinant human growth hormone on growth impariment (1991)
    Springer
    Pediatric nephrology 5 (1991), S. 603-606 
    Details
    ISSN: 1432-198X
    Keywords: Growth ; Corticosteroid ; Growth hormone ; Insulin-like growth factor-1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We evaluated the efficacy of recombinant human growth hormone (r-hGH) on corticosteroid (CS)-induced growth-impaired rats with proteinuria (passive Heymann nephritis). R-hGH (2 IU twice daily) improved growth in rats treated with 20 mg/kg per day of prednisolone succinate in our 4-week study. Although plasma hGH was significantly increased in rats treated with r-HGH, plasma insulin-like growth factor-1 levels were not different between treated and untreated rats. The food utilization rate was significantly improved by r-hGH. R-hGH did not affect proteinuria, renal function, or calcium and phosphate metabolism. Our results suggest that r-hGH may be effective in improving growth impairment due to CS administration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00856650
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