ISSN:
1617-4623
Keywords:
Glycine max
;
Seed storage protein
;
Gene regulation
;
Protein binding sequence
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The soybean embryo factor binding sequence in the glycinin A2B1a gene promoter was delimited to an A/T-rich 9 bp sequence, 5′-TAATAATTT-3′, designated as the glycinin box, by DNA footprinting and gel mobility shift assay using synthetic oligonucleotides. It was shown that the interaction with the factor takes place at a defined DNA sequence rather than at random A/T-rich sequence blocks in the glycinin 5′ flanking region. There are four glycinin boxes in the quantitative regulatory region between positions − 545 and − 378 of the glycinin A2B1a promoter. Multiple nonamer motifs similar to the glycinin box were also found in the equivalent regions of other glycinin and legumin promoters, suggesting that they must be conserved as a binding site for the embryo factor that activates the differential and stage-specific expression of seed 11S globulin genes in leguminous plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00301071
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