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Notes: Abstract The vast majority of proteins comprising the mitochondrion are encoded by nuclear genes, synthesized on ribosomes in the cytosol, and translocated into the various mitochondrial subcompartments. During this process proteins must cross the lipid membranes of the mitochondrion without interfering with the integrity or functions of the organelle. In recent years an approach combining biochemical, molecular, genetic, and morphological methodology has provided insights into various aspects of this complex process of intracellular protein sorting. In particular, a greater understanding of the molecular specificity and mechanism of targeting of mitochondrial preproteins has been reached, as a protein complex of the outer membrane which facilitates recognition and initial membrane insertion has been identified and characterized. Furthermore, pathways and components involved in the translocation of preproteins across the two mitochondrial membranes are being dissected and defined. The energetics of translocation and the processes of unfolding and folding of proteins during transmembrane transfer are closely linked to the function of a host of proteins known as heat-shock proteins or molecular chaperones, present both outside and inside the mitochondrion. In addition, the analysis of the process of folding of polypeptides in the mitochondrial matrix has allowed novel and unexpected insights into general pathways of protein folding assisted by folding factors. Pathways of sorting of proteins to the four different mitochondrial subcompartments — the outer membrane (OM), intermembrane space, inner membrane (IM) and matrix — are only partly understood and reveal an amazing complexity and variation. Many additional protein factors are involved in these latter processes, a few of which have been analyzed, such as cytochrome c heme lyase and cytochrome c 1 heme lyase, enzymes that catalyze the covalent addition of the heme group to cytochrome c and c 1 preproteins, and the mitochondrial processing peptidase which cleaves signal sequence after import of preproteins into the matrix. Thus, the study of transport of polypeptides through the mitochondrial membranes does not only contribute to the understanding of how biological membranes facilitate the penetration of macromolecules but also provides novel insights into the structure and function of this organelle. are being dissected and defined. The energetics of translocation and the processes of unfolding and folding of proteins during transmembrane transfer are closely linked to the function of a host of proteins known as heat-shock proteins or molecular chaperones, present both outside and inside the mitochondrion. In addition, the analysis of the process of folding of polypeptides in the mitochondrial matrix has allowed novel and unexpected insights into general pathways of protein folding assisted by folding factors. Pathways of sorting of proteins to the four different mitochondrial subcompartments — the outer membrane (OM), intermembrane space, inner membrane (IM) and matrix — are only partly understood and reveal an amazing complexity and variation. Many additional protein factors are involved in these latter processes, a few of which have been analyzed, such as cytochrome c heme lyase and cytochrome c 1 heme lyase, enzymes that catalyze the covalent addition of the heme group to cytochrome c and c 1 preproteins, and the mitochondrial processing peptidase which cleaves signal sequences after import of preproteins into the matrix. Thus, the study of transport of polypeptides through the mitochondrial membranes does not only contribute to the understanding of how biological membranes facilitate the penetration of macromolecules but also provides novel insights into the structure and function of this organelle.

Notes: Abstract X-ray spectra of the sun have been obtained during solar flares. New emission lines are observed in the spectral range from 1.3 Å to 3 Å, and 8 Å–20 Å, the most intense of the new emission features being tentatively attributed to optical transitions in high stages of ionization of iron (Fexxv through Fexx). Studies of the variability of these lines during flares provide new information of the development of a hot plasma in the initial stages of the flare event.

Notes: Abstract Theoretical line ratios involving 2s 2 S - 3p 2 P, 2p 2 P - 3s 2 S, and 2p 2 S - 3d 2 D transitions inCiv between 312 and 420 Å are presented. A comparison of these with solar active region observational data obtained during a rocket flight by the Solar EUV Rocket Telescope and Spectrograph (SERTS) reveals good agreement between theory and experiment, with discrepancies that average only 22%. This provides experimental support for the accuracy of the atomic data adopted in the line ratio calculations, and also resolves discrepancies found previously when the theoretical results were compared with solar data from the S082A instrument on boardSkylab. The potential usefulness of theCIV line ratios as electron temperature diagnostics for the solar transition region is briefly discussed.

Notes: Abstract The observation of extreme ultraviolet (EUV) emission lines of Fe ix through Fe xvi made by Orbiting Solar Observatory-1 are discussed and applied to a study of the solar corona above active regions. Ultraviolet and radio emission are determined and compared for several levels of activity classified according to the type of sunspot group associated with the active region. Both radio emission and line radiation from Fe xvi, the highest stage of ionization of Fe observed, are observed to increase rapidly with the onset of activity and are most intense over an E-spot group early in the lifetime of the active region. As activity diminishes, radiation from Fe xv and Fe xvi becomes relatively more prominent. The observations imply that the coronal temperature reaches a maximum during the period of highest activity, as indicated by sunspot-group complexity and the occurrence of chromospheric flares. A maximum coronal electron temperature of 4.0 × 106 °K is estimated when taking into account the mechanism of dielectronic recombination. Concurrently, the average coronal electron density increases by a factor of 10–12. Both electron temperature and density decrease as activity subsides. The coronal temperature above the remaining Ca ii plage is estimated to be 2.5–3.0 × 106 °K after flare activity has ceased and sunspots have disappeared.

Notes: [Auszug] The precursor of the ADP/ATP carrier, the most abundant protein of the mitochondrial inner membrane, was synthesized in rabbit reticulocyte lysate in the presence of 35S-methioninen. To accumulate the precursor at the mitochondrial outer membrane (receptor stage and GIP stage), isolated ...

Notes: [Auszug] The mitochondrial import receptors MOM19 and MOM72 form a complex with two other proteins of the mitochondrial outer membrane, MOM38 and MOM22. This receptor complex is involved in recognition, membrane insertion and translocation of precursor proteins with MOM38 constituting (at least part of) the ...