ISSN:
1432-1076
Keywords:
Insulin-dependent diabetes mellitus
;
Hypoglycaemia
;
β2-adrenoceptors
;
Cyclic adenosine monophosphate
;
Childhood
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Little is known about the receptor and post receptor mechanisms of sympathoadrenal signal transmission in type I diabetes mellitus. Therefore, we examined the maximum binding of granulocyte β2-adrenoceptors and the in vitro c-AMP accumulation in lymphocytes of 24 children and adolescents with diabetes mellitus and 14 similarly aged healthy subjects. The number of high affinity β2-adrenoceptors on granulocytes correlated significantly with unstimulated (r=0.6,P〈0.004) and with isoproterenol stimulated c-AMP values in lymphocytes (r=0.68,P〈0.0007) showing the proportional changes of β2-adrenoceptors and c-AMP in two different cells. The number of β2-adrenoceptors on granulocytes was significantly reduced in diabetic as compared to healthy children (median 1397, range 599–3405 vs. 2205, 825–3200 β2-adrenoceptors per granulocyte,P=0.014). Moreover, the percentage in vitro stimulation of c-AMP by isoproterenol in lymphocytes was significantly reduced in diabetic children as compared to healthy individuals (120%, 39%–278% vs. 225%, 66%–500%,P=0.012). These results indicate a decreased sympathoadrenergic signal transmission in peripheral blood cells as a model for the liver probably contributing to severe hypoglycaemia in diabetic children.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02073373
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