ISSN:
1619-7089
Keywords:
Immunoscintigraphy
;
Technetium 99m-labelled antibodies
;
CD4-specific (T-lymphocyte) antibodies
;
Rheumatoid arthritis
;
Localisation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract CD4 expressing T-lymphocytes are involved in the pathogenesis of rheumatoid arthritis, so the possibility of using radiolabelled CD4-specific antibodies to localise diseased joints was studied. Prospectively six patients with rheumatoid arthritis were investigated. Five of them received 200–300 μg of a 555 MBq technetium 99m CD4-specific antibody (MAX.16H5) and were examined with three phase bone scans. Max.16H5 (IgG1) was labelled according to the mercaptoethanol (Schwarz) method. Lymphocytes of one patient were isolated on a Ficoll-Hypaque gradient and labelled with the antibody in vitro. Scans were performed 1.5 h, 4 and 24 h post injection in anterior and posterior views. In all patients, diseased joints could be clearly imaged at as early as 1.5 h. The localisation of the diseased joints correlated (P〈0.01) with the clinical signs, with the early methylene diphosphonate (MDP) scan (P 〉 0.01) and only weakly with the late bone scan (P 〉 0.05). According to these data we conclude that99mTc-labelled CD4-specific antibodies specifically image actively diseased joints in rheumatoid arthritis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00811445
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