ISSN:
1432-1769
Keywords:
fluorescence imaging
;
confocal
;
median filtering
;
biomedical microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Computer Science
Notes:
Abstract A different way of processing confocally scanned fluorescence images is presented. Linear median hybrid methods and linear filtering methods are compared numerically with a conventionally processed artificial data set and with real confocal data. The use of linear median hybrid techniques reduces the time required for recording three-dimensional data sets with a confocal fluorescence microscope as well as the photo-damage to the biological sample. The implementation of a linear median algorithm on the hardware level of a confocal microscope is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01815301
Permalink
