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  • 1990-1994  (4)
  • 1905-1909
  • 1860-1869
  • 1850-1859
  • Analytical Chemistry and Spectroscopy  (3)
  • Cell culture  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Crosslinked gelatin beads: A culture method for the study of cell adhesion and invasion (1994)
    Springer
    Methods in cell science 16 (1994), S. 183-188 
    Details
    ISSN: 1573-0603
    Keywords: Beads ; Cell culture ; Degradation ; Fibronectin ; Gelatin ; Invasion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method is described for culturing invasive cell lines on crosslinked gelatin beads and preparing them for immunocytochemical and morphological observation. Very invasive cells such as Rous sarcoma virus-transformed chicken embryo fibroblasts, and human melanoma LOX and RPMI7951 and breast carcinoma MDA-MB-231 cells will actively degrade this matrix, extending cellular protrusions, called invadopodia, into the sites of degradation. Normal chicken embryo fibroblasts and other non-invasive cell lines do not disrupt the surface of these beads and do not form invadopodia. Invadopodia extending into the bead can be visualized by electron microscopy. Cellular removal of fluorescent fibronectin that has been covalently coupled to the bead surface can be monitored using fluorescence microscopy of frozen-thin-sections. In double label experiments, immunocytochemistry is used to localize antigens in invadopodia at sites of membrane invasion. The materials for bead preparation are inexpensive, and this method has the advantage that many cell types will attach and spread readily on the beads, while only highly invasive cells will invade into the bead.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01540647
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sequential proton saturation for the heteronuclear 1D NOE experiment (1992)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 30 (1992), S. 466-475 
    Details
    ISSN: 0749-1581
    Keywords: Heteronuclear NOE ; Selective pulses ; Shaped pulses ; Sequential excitation ; Improved data accumulation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An improved 1D pulse sequence for detecting heteronuclear NOEs on a qualitative level is described and demonstrated. It makes use of shaped selective pulses and takes advantage of sequential perturbation of several proton resonances during one experiment and correspondingly more efficient data accumulation schemes. The method is particularly suitable for assigning quaternary carbons and for establishing connectivities between assigned molecular fragments bound to these quaternary centres.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260300603
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Low-temperature study of dynamic effects in tetramethyllimonene (1990)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 28 (1990), S. 205-211 
    Details
    ISSN: 0749-1581
    Keywords: Tetramethyllimonene ; Assignments ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Dynamic effects of tetramethyllimonene were studied at low temperatures. Nuclear Overhauser effects measured under optimized conditions at 223 K and the results of 2D correlation experiments allowed the identification of two conformers and the assignment of all the proton and carbon resonances. For the determination of exchange and relaxation rates the method of saturation transfer was applied. At temperatures below 223 K further dynamic effects were recognized. Structural information obtained experimentally was compared with the results of Allinger force field calculations.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260280303
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Conformational analysis of cembrenenol: Application of 1H and 13C NMR spectroscopy and of computational methods (1991)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 29 (1991), S. 834-847 
    Details
    ISSN: 0749-1581
    Keywords: Cembrenenol ; Cyclic diterpene ; Conformational analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The solution structure of the cyclic diterpene cembrenenol was investigated. A strategy for conformational analysis is proposed which is based on computational methods and experiments to determine the structure-relevant NMR parameters. Computed and experimentally determined steady-state NOEs were compared in a first step, which served to sort out a few probable conformers. The detailed analysis of homo- and hetero-nuclear coupling information together with NOE data defining local geometries allowed, in a second step, the selection of the most probable conformation. The structure corresponds to the lowest energy structure proposed by computational methods alone. Its ring structure is in excellent agreement with the x-ray ring structure of the closely related cembrenene.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260290815
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    Paper (German National Licenses)
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